Abstract
Phenol suifotransferase (PST) catalyzes the sulfate conjugation of catecholamines and of phenolic drugs. Human platelet PST exists in at least a thermolabile form (TL PST) and a thermostable form (TS PST). The mean basal level of platelet TS PST activity in samples from American blacks is significantly higher than the basal activity in samples from whites. We carried out the studies reported here to determine whether the higher basal TS PST activity in platelet homogenates from blacks was biochemically similar to the lower basal activity in samples from whites. We also characterized variations in TS PST thermal stability. Platelet TS PST activities in samples from the two groups were almost identical with respect to pH optima, Michaells-Menten constant values for substrates, and susceptibilities to inhibition by 2,6-dichloro-4-nitrophenol and sodium chloride. Thermolabile and thermostable TS PST were present in samples from both blacks and whites. Thermal stabilities of TS PST in samples from 167 volunteers 104 blacks, 63 whites) were expressed as heated sample-to-control sample ratios. Bimodal frequency distribution histograms of the heated-to-control ratios revealed subgroups of samples with thermolabile TS PST activities from 13.8% of blacks (heated-to-control ratio
| Original language | English |
|---|---|
| Pages (from-to) | 773-783 |
| Number of pages | 11 |
| Journal | The Journal of Laboratory and Clinical Medicine |
| Volume | 112 |
| Issue number | 6 |
| State | Published - 1988 |
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All Science Journal Classification (ASJC) codes
- Medicine(all)
- Pathology and Forensic Medicine
Cite this
Human platelet thermostable phenol sulfotransferase from blacks and whites : Biochemical properties and variations in thermal stability. / Anderson, Robert J.; Jackson, Bertha L.; Liebentritt, Dianne K.
In: The Journal of Laboratory and Clinical Medicine, Vol. 112, No. 6, 1988, p. 773-783.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human platelet thermostable phenol sulfotransferase from blacks and whites
T2 - Biochemical properties and variations in thermal stability
AU - Anderson, Robert J.
AU - Jackson, Bertha L.
AU - Liebentritt, Dianne K.
PY - 1988
Y1 - 1988
N2 - Phenol suifotransferase (PST) catalyzes the sulfate conjugation of catecholamines and of phenolic drugs. Human platelet PST exists in at least a thermolabile form (TL PST) and a thermostable form (TS PST). The mean basal level of platelet TS PST activity in samples from American blacks is significantly higher than the basal activity in samples from whites. We carried out the studies reported here to determine whether the higher basal TS PST activity in platelet homogenates from blacks was biochemically similar to the lower basal activity in samples from whites. We also characterized variations in TS PST thermal stability. Platelet TS PST activities in samples from the two groups were almost identical with respect to pH optima, Michaells-Menten constant values for substrates, and susceptibilities to inhibition by 2,6-dichloro-4-nitrophenol and sodium chloride. Thermolabile and thermostable TS PST were present in samples from both blacks and whites. Thermal stabilities of TS PST in samples from 167 volunteers 104 blacks, 63 whites) were expressed as heated sample-to-control sample ratios. Bimodal frequency distribution histograms of the heated-to-control ratios revealed subgroups of samples with thermolabile TS PST activities from 13.8% of blacks (heated-to-control ratio
AB - Phenol suifotransferase (PST) catalyzes the sulfate conjugation of catecholamines and of phenolic drugs. Human platelet PST exists in at least a thermolabile form (TL PST) and a thermostable form (TS PST). The mean basal level of platelet TS PST activity in samples from American blacks is significantly higher than the basal activity in samples from whites. We carried out the studies reported here to determine whether the higher basal TS PST activity in platelet homogenates from blacks was biochemically similar to the lower basal activity in samples from whites. We also characterized variations in TS PST thermal stability. Platelet TS PST activities in samples from the two groups were almost identical with respect to pH optima, Michaells-Menten constant values for substrates, and susceptibilities to inhibition by 2,6-dichloro-4-nitrophenol and sodium chloride. Thermolabile and thermostable TS PST were present in samples from both blacks and whites. Thermal stabilities of TS PST in samples from 167 volunteers 104 blacks, 63 whites) were expressed as heated sample-to-control sample ratios. Bimodal frequency distribution histograms of the heated-to-control ratios revealed subgroups of samples with thermolabile TS PST activities from 13.8% of blacks (heated-to-control ratio
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M3 - Article
VL - 112
SP - 773
EP - 783
JO - Translational Research
JF - Translational Research
SN - 1931-5244
IS - 6
ER -