Human salivary gland-specific daily variations in histatin concentrations determined by a novel quantitation technique

Heloisa Gusman, Cataldo Leone, Eva J. Helmerhorst, Martha E. Nunn, Bianca Flora, Robert F. Troxler, Frank G. Oppenheim

Research output: Contribution to journalArticle

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Abstract

Histatins constitute a distinct family of human salivary antimicrobial peptides, of which histatins 1, 3 and 5 are the most abundant. To evaluate salivary gland-specific differences in histatin secretion, we used the recently developed histatin-zinc precipitation method to quantify histatins and to assess daily variations in secretions. Stimulated pure secretions from parotid glands (HPS) and submandibular/sublingual glands (SMSL) were collected from 10 different subjects at four different times of the day (9:35 a.m.; 12:40 p.m.; 2:50 p.m. and 5:00 p.m.). Zinc precipitation and subsequent reversed phase HPLC analysis were performed to determine concentrations of histatins 1, 3 and 5 with reference to purified histatin standards. Both HPS and SMSL secretions displayed daily variations in histatin concentrations. HPS values showed a maximum at mid-day and SMSL samples showed a maximum in the morning. Mean daily histatin concentrations were almost three fold higher in SMSL than in HPS. Mean histatin 1, 3 and 5 concentrations in HPS from 10 subjects ranged from 0.7 to 2.8, 0.6 to 4.3 and 1.0 to 4.3mg%, respectively. The corresponding means in SMSL were 2.8-12.2, 1.5-7.5 and 2.6-9.0mg%, respectively. Remarkably, although histatins constitute only 3-10% of total protein in these secretions, an almost perfect correlation between total protein and total histatin concentrations was observed for both glands. Despite a broad range in histatin concentrations between individuals, this study demonstrated a hitherto unidentified daily variation in histatin concentrations in HPS and SMSL secretions and a differential expression pattern which might have functional implications.

Original languageEnglish
Pages (from-to)11-22
Number of pages12
JournalArchives of Oral Biology
Volume49
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

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Histatins
Salivary Glands
Sublingual Gland
Submandibular Gland
Zinc
Salivary Proteins and Peptides

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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Human salivary gland-specific daily variations in histatin concentrations determined by a novel quantitation technique. / Gusman, Heloisa; Leone, Cataldo; Helmerhorst, Eva J.; Nunn, Martha E.; Flora, Bianca; Troxler, Robert F.; Oppenheim, Frank G.

In: Archives of Oral Biology, Vol. 49, No. 1, 01.2004, p. 11-22.

Research output: Contribution to journalArticle

Gusman, Heloisa ; Leone, Cataldo ; Helmerhorst, Eva J. ; Nunn, Martha E. ; Flora, Bianca ; Troxler, Robert F. ; Oppenheim, Frank G. / Human salivary gland-specific daily variations in histatin concentrations determined by a novel quantitation technique. In: Archives of Oral Biology. 2004 ; Vol. 49, No. 1. pp. 11-22.
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