TY - JOUR
T1 - Hybrid interpenetrating hydrogel network favoring the bidirectional migration of tenocytes for rotator cuff tendon regeneration
AU - Thankam, Finosh G.
AU - Diaz, Connor
AU - Chandra, Isaiah
AU - Link, Josh
AU - Newton, Joseph
AU - Dilisio, Matthew F.
AU - Agrawal, Devendra K.
N1 - Funding Information:
This work was supported by LB506 grant to D.K. Agrawal and LB606 to M.F. Dilisio from the State of Nebraska. The research work of D.K. Agrawal is also supported by grants R01 HL144125 and R01 HL147662 from the National Institutes of Health. The contents in this original research article are solely the responsibility of the authors and do not necessarily represent the official views of the National Institutes of Health or the State of Nebraska. The authors would like to thank the staffs and scientists at the University of Nebraska Medical Center for technical assistance for SEM and AFM analysis.
Publisher Copyright:
© 2021 Wiley Periodicals LLC.
PY - 2022/2
Y1 - 2022/2
N2 - Replenishment of tenocytes to the injury site is an ideal strategy to improve healing response and accelerate the tendon ECM regeneration. The present study focused on the synthesis and characterization of a hybrid hydrogel scaffold system poly(propylene-fumarate)-alginate-polyvinyl alcohol-acrylic acid (PAPA) using poly(propylene-fumarate) (PPF), alginate, polyvinyl alcohol (PVA) and acrylic acid and the in vitro investigation of bidirectional mobility of swine shoulder tenocytes (SST) for its potential application in rotator-cuff tendon regeneration. IR analysis revealed the presence of alginate, PPF and PVA segments on the surface, SEM and AFM analyses revealed the porous and nano-topographical features of PAPA, respectively, swelling was 712.6 ± 84.21% with the EWC (%) of 87.59 ± 1.26 having the diffusional exponent and swelling constant 0.551 and 1.8, respectively. PAPA was biodegradable, cytocompatible and supported long-term survival of SSTs. SEM imaging revealed the adhesion, colonization, and sheet formation of SSTs within the PAPA hydrogel network. The SSTs seeded on the PAPA scaffolds were peculiar for their bidirectional migration as the anterograde movement was completed in 9 days whereas the retrograde infiltration occurred up to the depth of 198 μm. These findings suggest the promising translational potential of PAPA scaffold system in the management of rotator cuff tendon injury.
AB - Replenishment of tenocytes to the injury site is an ideal strategy to improve healing response and accelerate the tendon ECM regeneration. The present study focused on the synthesis and characterization of a hybrid hydrogel scaffold system poly(propylene-fumarate)-alginate-polyvinyl alcohol-acrylic acid (PAPA) using poly(propylene-fumarate) (PPF), alginate, polyvinyl alcohol (PVA) and acrylic acid and the in vitro investigation of bidirectional mobility of swine shoulder tenocytes (SST) for its potential application in rotator-cuff tendon regeneration. IR analysis revealed the presence of alginate, PPF and PVA segments on the surface, SEM and AFM analyses revealed the porous and nano-topographical features of PAPA, respectively, swelling was 712.6 ± 84.21% with the EWC (%) of 87.59 ± 1.26 having the diffusional exponent and swelling constant 0.551 and 1.8, respectively. PAPA was biodegradable, cytocompatible and supported long-term survival of SSTs. SEM imaging revealed the adhesion, colonization, and sheet formation of SSTs within the PAPA hydrogel network. The SSTs seeded on the PAPA scaffolds were peculiar for their bidirectional migration as the anterograde movement was completed in 9 days whereas the retrograde infiltration occurred up to the depth of 198 μm. These findings suggest the promising translational potential of PAPA scaffold system in the management of rotator cuff tendon injury.
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U2 - 10.1002/jbm.b.34924
DO - 10.1002/jbm.b.34924
M3 - Article
C2 - 34342931
AN - SCOPUS:85111831320
VL - 110
SP - 467
EP - 477
JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials
JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials
SN - 0021-9304
IS - 2
ER -