Identification, cloning, and expression of human estrogen receptor-α36, a novel variant of human estrogen receptor-α66

Yi Wang Zhao, XinTian Zhang, Peng Shen, Brian W. Loggie, YunChao Chang, Thomas F. Deuel

Research output: Contribution to journalArticle

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Abstract

The identification and subsequent cloning of the 66-kDa human estrogen receptor (here termed hER-α66), its 46-kDa splice variant hER-α46, and the closely related hER-β have had a profound impact on the generation of new understanding of estrogen-mediated functions and led to progress in diagnosis and treatment of human breast cancer. However, a persistent problem has been that not all findings previously reported in estrogen-stimulated cell proliferation can be explained through the known properties of the different estrogen receptors described. As the consequence of a search for alternative mechanisms to account for these different findings, we have now identified, cloned, and expressed in HEK 293 cells a previously unrecognized 36-kDa variant of hER-α66, termed hER-α36. hER-α36 differs from hER-α66 since it lacks both transcriptional activation domains (AF-1 and AF-2) but it retains the DNA-binding domain, and partial dimerization and ligand-binding domains of hER-α66. It also contains three myristoylation sites postulated to direct ER-α36 to the plasma membrane. It is concluded that ER-α36 is a unique variant of ER-α66; ER-α36 is predicted to function as a dominant-negative effector of hER-α66-mediated estrogen-responsive gene pathways and has the potential to trigger membrane-initiated mitogenic estrogen signaling.

Original languageEnglish
Pages (from-to)1023-1027
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume336
Issue number4
DOIs
StatePublished - Nov 4 2005

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Cloning
Estrogen Receptors
Organism Cloning
Estrogens
Furylfuramide
Dimerization
HEK293 Cells
Cell proliferation
Cell membranes
Transcriptional Activation
Genes
Chemical activation
Cell Proliferation
Cell Membrane
Breast Neoplasms
Ligands
Membranes
DNA
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification, cloning, and expression of human estrogen receptor-α36, a novel variant of human estrogen receptor-α66. / Zhao, Yi Wang; Zhang, XinTian; Shen, Peng; Loggie, Brian W.; Chang, YunChao; Deuel, Thomas F.

In: Biochemical and Biophysical Research Communications, Vol. 336, No. 4, 04.11.2005, p. 1023-1027.

Research output: Contribution to journalArticle

Zhao, Yi Wang ; Zhang, XinTian ; Shen, Peng ; Loggie, Brian W. ; Chang, YunChao ; Deuel, Thomas F. / Identification, cloning, and expression of human estrogen receptor-α36, a novel variant of human estrogen receptor-α66. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 336, No. 4. pp. 1023-1027.
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