Identification of a BRCA2-Specific Modifier Locus at 6p24 Related to Breast Cancer Risk

Mia M. Gaudet, Karoline B. Kuchenbaecker, Joseph Vijai, Robert J. Klein, Tomas Kirchhoff, Lesley McGuffog, Daniel Barrowdale, Alison M. Dunning, Andrew Lee, Joe Dennis, Sue Healey, Ed Dicks, Penny Soucy, Olga M. Sinilnikova, Vernon S. Pankratz, Xianshu Wang, Ronald C. Eldridge, Daniel C. Tessier, Daniel Vincent, Francois BacotFrans B.L. Hogervorst, Susan Peock, Dominique Stoppa-Lyonnet, Florence Coulet, Chrystelle Colas, Florent Soubrier, Paolo Peterlongo, Rita K. Schmutzler, Katherine L. Nathanson, Marion Piedmonte, Christian F. Singer, Mads Thomassen, Johanna Sokolowska, Myriam Bronner, Thomas V.O. Hansen, Susan L. Neuhausen, Ignacio Blanco, Mark H. Greene, Judith Garber, Jeffrey N. Weitzel, Irene L. Andrulis, David E. Goldgar, Emma D'Andrea, Trinidad Caldes, Heli Nevanlinna, Ana Osorio, Elizabeth J. van Rensburg, Adalgeir Arason, Gad Rennert, Ans M.W. van den Ouweland, Annemarie H. van der Hout, Carolien M. Kets, Cora M. Aalfs, Juul T. Wijnen, Margreet G.E.M. Ausems, Debra Frost, Steve Ellis, Elena Fineberg, Radka Platte, D. Gareth Evans, Chris Jacobs, Julian Adlard, Marc Tischkowitz, Mary E. Porteous, Francesca Damiola, Lisa Golmard, Laure Barjhoux, Michel Longy, Muriel Belotti, Sandra Fert Ferrer, Sylvie Mazoyer, Amanda B. Spurdle, Siranoush Manoukian, Monica Barile, Maurizio Genuardi, Norbert Arnold, Alfons Meindl, Christian Sutter, Barbara Wappenschmidt, Susan M. Domchek, Georg Pfeiler, Eitan Friedman, Uffe Birk Jensen, Mark Robson, Sohela Shah, Conxi Lazaro, Phuong L. Mai, Javier Benitez, Melissa C. Southey, M. K. Schmidt, Peter A. Fasching, Julian Peto, Manjeet K. Humphreys, Qin Wang, Kyriaki Michailidou, Elinor J. Sawyer, Barbara Burwinkel, Pascal Guénel, Stig E. Bojesen, Roger L. Milne, Hermann Brenner, Magdalena Lochmann, Hiltrud Brauch, Yon Dschun Ko, Christian Baisch, Hand Peter Fischer, Thomas Bruening, Beate Pesch, Sylvia Rabstein, Anne Spickenheuer, Kristiina Aittomäki, Thilo Dörk, Sara Margolin, Arto Mannermaa, Diether Lambrechts, Jenny Chang-Claude, Paolo Radice, Graham G. Giles, Christopher A. Haiman, Robert Winqvist, Peter Devillee, Montserrat García-Closas, Nils Schoof, M. J. Hooning, Angela Cox, Paul D.P. Pharoah, Anna Jakubowska, Nick Orr, Anna González-Neira, Guillermo Pita, M. Rosario Alonso, Per Hall, Fergus J. Couch, Jacques Simard, David Altshuler, Douglas F. Easton, Georgia Chenevix-Trench, Antonis C. Antoniou, Kenneth Offit, M. A. Rookus, F. E. van Leeuwen, S. Verhoef, J. L. de Lange, J. M. Collée, C. Seynaeve, C. H.M. van Deurzen, C. J. van Asperen, R. A. Tollenaar, P. Devilee, T. C.T.E.F. van Cronenburg, A. R. Mensenkamp, R. B. van der Luijt, T. A.M. van Os, J. J.P. Gille, Q. Waisfisz, H. E.J. Meijers-Heijboer, E. B. Gómez-Garcia, M. J. Blok, J. C. Oosterwijk, M. J. Mourits, G. H. de Bock, H. F. Vasen, Zosia Miedzybrodzka, Helen Gregory, Patrick Morrison, Lisa Jeffers, Trevor Cole, Kai ren Ong, Jonathan Hoffman, Alan Donaldson, Margaret James, Joan Paterson, Amy Taylor, Alexandra Murray, Mark T. Rogers, Emma McCann, M. John Kennedy, David Barton, Mary Porteous, Sarah Drummond, Carole Brewer, Emma Kivuva, Anne Searle, Selina Goodman, Kathryn Hill, Rosemarie Davidson, Victoria Murday, Nicola Bradshaw, Lesley Snadden, Mark Longmuir, Catherine Watt, Sarah Gibson, Eshika Haque, Ed Tobias, Alexis Duncan, Louise Izatt, Caroline Langman, Angela Brady, Huw Dorkins, Athalie Melville, Kashmir Randhawa, Julian Barwell, Gemma Serra-Feliu, Ian Ellis, Catherine Houghton, Fiona Lalloo, Jane Taylor, Lucy Side, Alison Male, Cheryl Berlin, Jacqueline Eason, Fiona Douglas, Oonagh Claber, Rebecca Collier, Irene Jobson, Lisa Walker, Diane McLeod, Sarah Durell, Barbara Stayner, Rosalind A. Eeles, Susan Shanley, Nazneen Rahman, Richard Houlston, Elizabeth Bancroft, Elizabeth Page, Audrey Ardern-Jones, Kelly Kohut, Jennifer Wiggins, Elena Castro, Emma Killick, Sue Martin, Gillian Rea, Anjana Kulkarni, Jackie Cook, Oliver Quarrell, Cathryn Bardsley, Shirley Hodgson, Sheila Goff, Glen Brice, Lizzie Winchester, Charlotte Eddy, Vishakha Tripathi, Virginia Attard, Anna Lehmann, Diana Eccles, Anneke Lucassen, Gillian Crawford, Donna McBride, Sarah Smalley, Olga Sinilnikova, Carole Verny-Pierre, Sophie Giraud, Mélanie Léone, Marion Gauthier-Villars, Bruno Buecher, Claude Houdayer, Virginie Moncoutier, Carole Tirapo, Antoine de Pauw, Brigitte Bressac-de-Paillerets, Olivier Caron, Yves Jean Bignon, Nancy Uhrhammer, Christine Lasset, Valérie Bonadona, Sandrine Handallou, Agnés Hardouin, Pascaline Berthet, Hagay Sobol, Violaine Bourdon, Tetsuro Noguchi, Audrey Remenieras, Isabelle Coupier, Pascal Pujol, Jean Philippe Peyrat, Joëlle Fournier, Françoise Révillion, Philippe Vennin, Claude Adenis, Etienne Rouleau, Rosette Lidereau, Liliane Demange, Catherine Nogues, Danièle Muller, Jean Pierre Fricker, Emmanuelle Barouk-Simonet, Françoise Bonnet, Virginie Bubien, Nicolas Sevenet, Christine Toulas, Rosine Guimbaud, Laurence Gladieff, Viviane Feillel, Hélène Dreyfus, Christine Rebischung, Magalie Peysselon, Fanny Coron, Laurence Faivre, Fabienne Prieur, Marine Lebrun, Caroline Kientz, Marc Frénay, Laurence Vénat-Bouvet, Capucine Delnatte, Isabelle Mortemousque, Henry T. Lynch, Carrie L. Snyder

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

Common genetic variants contribute to the observed variation in breast cancer risk for BRCA2 mutation carriers; those known to date have all been found through population-based genome-wide association studies (GWAS). To comprehensively identify breast cancer risk modifying loci for BRCA2 mutation carriers, we conducted a deep replication of an ongoing GWAS discovery study. Using the ranked P-values of the breast cancer associations with the imputed genotype of 1.4 M SNPs, 19,029 SNPs were selected and designed for inclusion on a custom Illumina array that included a total of 211,155 SNPs as part of a multi-consortial project. DNA samples from 3,881 breast cancer affected and 4,330 unaffected BRCA2 mutation carriers from 47 studies belonging to the Consortium of Investigators of Modifiers of BRCA1/2 were genotyped and available for analysis. We replicated previously reported breast cancer susceptibility alleles in these BRCA2 mutation carriers and for several regions (including FGFR2, MAP3K1, CDKN2A/B, and PTHLH) identified SNPs that have stronger evidence of association than those previously published. We also identified a novel susceptibility allele at 6p24 that was inversely associated with risk in BRCA2 mutation carriers (rs9348512; per allele HR = 0.85, 95% CI 0.80-0.90, P = 3.9×10-8). This SNP was not associated with breast cancer risk either in the general population or in BRCA1 mutation carriers. The locus lies within a region containing TFAP2A, which encodes a transcriptional activation protein that interacts with several tumor suppressor genes. This report identifies the first breast cancer risk locus specific to a BRCA2 mutation background. This comprehensive update of novel and previously reported breast cancer susceptibility loci contributes to the establishment of a panel of SNPs that modify breast cancer risk in BRCA2 mutation carriers. This panel may have clinical utility for women with BRCA2 mutations weighing options for medical prevention of breast cancer.

Original languageEnglish (US)
Article numbere1003173
JournalPLoS genetics
Volume9
Issue number3
DOIs
StatePublished - 2013

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

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    Gaudet, M. M., Kuchenbaecker, K. B., Vijai, J., Klein, R. J., Kirchhoff, T., McGuffog, L., Barrowdale, D., Dunning, A. M., Lee, A., Dennis, J., Healey, S., Dicks, E., Soucy, P., Sinilnikova, O. M., Pankratz, V. S., Wang, X., Eldridge, R. C., Tessier, D. C., Vincent, D., ... Snyder, C. L. (2013). Identification of a BRCA2-Specific Modifier Locus at 6p24 Related to Breast Cancer Risk. PLoS genetics, 9(3), [e1003173]. https://doi.org/10.1371/journal.pgen.1003173