Identification of calcium antagonist receptor binding sites using (3H)nitrendipine in bovine tracheal smooth muscle membranes

J. B. Cheng; A. Bewtra; R. G. Townley

doi:10.1007/BF01947575

Identification of calcium antagonist receptor binding sites using (³H)nitrendipine in bovine tracheal smooth muscle membranes

J. B. Cheng, A. Bewtra, R. G. Townley

Department of Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

(³H)Nitrendipine binding to the bovine tracheal muscle membrane at 25°C was rapid, saturable (B_max=14.8±3.9fmol/mg protein) and of high affinity (K_d=0.15±0.04 nM). The rank order of Ca²⁺ antagonists competing for airway (³H)nitrendipine binding was nitrendipine {Mathematical expression} nisoldipine {Mathematical expression} nifedipine » verapamil. Cromolyn, however, neither inhibited nor increased the binding.

Original language	English (US)
Pages (from-to)	267-269
Number of pages	3
Journal	Experientia
Volume	40
Issue number	3
DOIs	https://doi.org/10.1007/BF01947575
State	Published - Mar 1 1984

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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10.1007/BF01947575

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author = "Cheng, {J. B.} and A. Bewtra and Townley, {R. G.}",

year = "1984",

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AU - Cheng, J. B.

AU - Bewtra, A.

AU - Townley, R. G.

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AB - (3H)Nitrendipine binding to the bovine tracheal muscle membrane at 25°C was rapid, saturable (Bmax=14.8±3.9fmol/mg protein) and of high affinity (Kd=0.15±0.04 nM). The rank order of Ca2+ antagonists competing for airway (3H)nitrendipine binding was nitrendipine {Mathematical expression} nisoldipine {Mathematical expression} nifedipine » verapamil. Cromolyn, however, neither inhibited nor increased the binding.

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