Identification of calcium antagonist receptor binding sites using (3H)nitrendipine in bovine tracheal smooth muscle membranes

J. B. Cheng; Againdra K. Bewtra; R. G. Townley

doi:10.1007/BF01947575

Identification of calcium antagonist receptor binding sites using (3H)nitrendipine in bovine tracheal smooth muscle membranes

J. B. Cheng, Againdra K. Bewtra, R. G. Townley

Department of Medicine

Research output: Contribution to journal › Article

9 Scopus citations

Abstract

(³H)Nitrendipine binding to the bovine tracheal muscle membrane at 25°C was rapid, saturable (B_max=14.8±3.9fmol/mg protein) and of high affinity (K_d=0.15±0.04 nM). The rank order of Ca²⁺ antagonists competing for airway (³H)nitrendipine binding was nitrendipine {Mathematical expression} nisoldipine {Mathematical expression} nifedipine » verapamil. Cromolyn, however, neither inhibited nor increased the binding.

Original language	English
Pages (from-to)	267-269
Number of pages	3
Journal	Experientia
Volume	40
Issue number	3
DOIs	https://doi.org/10.1007/BF01947575
Publication status	Published - Mar 1984

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All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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Cheng, J. B., Bewtra, A. K., & Townley, R. G. (1984). Identification of calcium antagonist receptor binding sites using (3H)nitrendipine in bovine tracheal smooth muscle membranes. Experientia, 40(3), 267-269. https://doi.org/10.1007/BF01947575

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10.1007/BF01947575