Identification of calcium antagonist receptor binding sites using (3H)nitrendipine in bovine tracheal smooth muscle membranes

J. B. Cheng; A. Bewtra; R. G. Townley

doi:10.1007/BF01947575

Identification of calcium antagonist receptor binding sites using (³H)nitrendipine in bovine tracheal smooth muscle membranes

J. B. Cheng, A. Bewtra, R. G. Townley

Department of Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

(³H)Nitrendipine binding to the bovine tracheal muscle membrane at 25°C was rapid, saturable (B_max=14.8±3.9fmol/mg protein) and of high affinity (K_d=0.15±0.04 nM). The rank order of Ca²⁺ antagonists competing for airway (³H)nitrendipine binding was nitrendipine {Mathematical expression} nisoldipine {Mathematical expression} nifedipine » verapamil. Cromolyn, however, neither inhibited nor increased the binding.

Original language	English (US)
Pages (from-to)	267-269
Number of pages	3
Journal	Experientia
Volume	40
Issue number	3
DOIs	https://doi.org/10.1007/BF01947575
State	Published - Mar 1 1984

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1007/BF01947575

Fingerprint Dive into the research topics of 'Identification of calcium antagonist receptor binding sites using (<sup>3</sup>H)nitrendipine in bovine tracheal smooth muscle membranes'. Together they form a unique fingerprint.

Cite this

@article{ff4fee880e354444b723f0eabe1f3d01,

title = "Identification of calcium antagonist receptor binding sites using (3H)nitrendipine in bovine tracheal smooth muscle membranes",

abstract = "(3H)Nitrendipine binding to the bovine tracheal muscle membrane at 25°C was rapid, saturable (Bmax=14.8±3.9fmol/mg protein) and of high affinity (Kd=0.15±0.04 nM). The rank order of Ca2+ antagonists competing for airway (3H)nitrendipine binding was nitrendipine {Mathematical expression} nisoldipine {Mathematical expression} nifedipine » verapamil. Cromolyn, however, neither inhibited nor increased the binding.",

author = "Cheng, {J. B.} and A. Bewtra and Townley, {R. G.}",

year = "1984",

month = mar,

day = "1",

doi = "10.1007/BF01947575",

language = "English (US)",

volume = "40",

pages = "267--269",

journal = "Cellular and Molecular Life Sciences",

issn = "1420-682X",

publisher = "Birkhauser Verlag Basel",

number = "3",

}

TY - JOUR

T1 - Identification of calcium antagonist receptor binding sites using (3H)nitrendipine in bovine tracheal smooth muscle membranes

AU - Cheng, J. B.

AU - Bewtra, A.

AU - Townley, R. G.

PY - 1984/3/1

Y1 - 1984/3/1

N2 - (3H)Nitrendipine binding to the bovine tracheal muscle membrane at 25°C was rapid, saturable (Bmax=14.8±3.9fmol/mg protein) and of high affinity (Kd=0.15±0.04 nM). The rank order of Ca2+ antagonists competing for airway (3H)nitrendipine binding was nitrendipine {Mathematical expression} nisoldipine {Mathematical expression} nifedipine » verapamil. Cromolyn, however, neither inhibited nor increased the binding.

AB - (3H)Nitrendipine binding to the bovine tracheal muscle membrane at 25°C was rapid, saturable (Bmax=14.8±3.9fmol/mg protein) and of high affinity (Kd=0.15±0.04 nM). The rank order of Ca2+ antagonists competing for airway (3H)nitrendipine binding was nitrendipine {Mathematical expression} nisoldipine {Mathematical expression} nifedipine » verapamil. Cromolyn, however, neither inhibited nor increased the binding.

UR - http://www.scopus.com/inward/record.url?scp=0021354673&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021354673&partnerID=8YFLogxK

U2 - 10.1007/BF01947575

DO - 10.1007/BF01947575

M3 - Article

C2 - 6321228

AN - SCOPUS:0021354673

VL - 40

SP - 267

EP - 269

JO - Cellular and Molecular Life Sciences

JF - Cellular and Molecular Life Sciences

SN - 1420-682X

IS - 3