Identification of leukotriene D4 receptor binding sites in guinea pig lung homogenates using [3H]leukotriene D4

John B. Cheng, Robert G. Townley

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Abstract

We have characterized [3H]leukotriene D4 binding to guinea pig lung homogenates. Both biphasic dissociation kinetics and curvilinear Scatchard plots indicated the presence of [3H]leukotriene high and low affinity states of the binding sites. The rank order of potency for the competition study was leukotriene C4 = leukotriene D4 leukotriene E4 ≫ arachidonic acid, and for their contractile effect on lung strips was leukotriene C4 = leukotriene D4 = leukotriene E4 ≫ arachidonic acid. FPL-55712 was the only other agent tested that inhibited binding. These results suggest that binding of [3H]leukotriene D4 to the homogenate is consistent with its binding to specific leukotriene D4 receptor sites.

Original languageEnglish (US)
Pages (from-to)20-26
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume118
Issue number1
DOIs
StatePublished - Jan 13 1984

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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