Identification of muscarinic receptor subtypes in mouse parotid gland

Eileen L. Watson, Peter W. Abel, Dennis Dijulio, W. Zeng, M. Makoid, Kerry L. Jacobson, Lincoln T. Potter, Frank J. Dowd

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Immunoprecipitation of muscarinic receptors from mouse parotid membranes by specific subtype antisera showed that M3 and M1 receptors represented 75 and 15% of the total number of precipitable receptors, respectively. [N- methyl-3H]methylscopolamine (NMS) labeled a single class of high-affinity binding sites in membranes from parotid glands with a dissociation constant of 0.67 ± 0.02 nM and a maximum binding capacity of 176 ± 15 fmol/mg protein. Competition curves for NMS, atropine, 4-diphenylacetoxy-N- methylpiperidine methiodide (4-DAMP) and para-fluoro-hexahydro-sila-difenidol fit best to a one-site binding model, whereas pirenzepine and methoctramine fit best to a two-site binding model, indicating 76-90% M3 receptors. Results from the use of pirenzepine indicated that the second mouse parotid receptor subtype, unlike that of the submandibular gland, has atypical characteristics for an M1 receptor. The rank order of potency of muscarinic antagonists in inhibiting phosphoinositide turnover and biphasic effects of carbachol on isoproterenol-stimulated adenosine 3',5'cyclic monophosphate (cAMP) accumulation was atropine ≤ 4-DAMP >> pirenzepine > AF-DX 116. A specific M1 antagonist, m1-toxin, had no effect on carbachol augmentation or inhibition of isoproterenol responses. Results suggest that M3 receptors couple to both augmentation and inhibition of stimulated cAMP levels.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume271
Issue number3 40-3
StatePublished - Sep 1996
Externally publishedYes

Fingerprint

Pirenzepine
Parotid Gland
Muscarinic Receptors
Binding Sites
Carbachol
Atropine
Isoproterenol
Membranes
Muscarinic Antagonists
Submandibular Gland
Phosphatidylinositols
Immunoprecipitation
Cyclic AMP
Immune Sera
Proteins
4-diphenylacetoxy-1,1-dimethylpiperidinium

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Watson, E. L., Abel, P. W., Dijulio, D., Zeng, W., Makoid, M., Jacobson, K. L., ... Dowd, F. J. (1996). Identification of muscarinic receptor subtypes in mouse parotid gland. American Journal of Physiology - Cell Physiology, 271(3 40-3).

Identification of muscarinic receptor subtypes in mouse parotid gland. / Watson, Eileen L.; Abel, Peter W.; Dijulio, Dennis; Zeng, W.; Makoid, M.; Jacobson, Kerry L.; Potter, Lincoln T.; Dowd, Frank J.

In: American Journal of Physiology - Cell Physiology, Vol. 271, No. 3 40-3, 09.1996.

Research output: Contribution to journalArticle

Watson, EL, Abel, PW, Dijulio, D, Zeng, W, Makoid, M, Jacobson, KL, Potter, LT & Dowd, FJ 1996, 'Identification of muscarinic receptor subtypes in mouse parotid gland', American Journal of Physiology - Cell Physiology, vol. 271, no. 3 40-3.
Watson EL, Abel PW, Dijulio D, Zeng W, Makoid M, Jacobson KL et al. Identification of muscarinic receptor subtypes in mouse parotid gland. American Journal of Physiology - Cell Physiology. 1996 Sep;271(3 40-3).
Watson, Eileen L. ; Abel, Peter W. ; Dijulio, Dennis ; Zeng, W. ; Makoid, M. ; Jacobson, Kerry L. ; Potter, Lincoln T. ; Dowd, Frank J. / Identification of muscarinic receptor subtypes in mouse parotid gland. In: American Journal of Physiology - Cell Physiology. 1996 ; Vol. 271, No. 3 40-3.
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