Identification of novel alternatively spliced isoforms of the tropomyosin-encoding gene, TMnm, in the rat cochlea

Kirk Beisel, Jane E. Kennedy

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Analysis of a rat cochlear cDNA library for the expression of the non-muscle (nm) tropomyosin (TM)-encoding gene (TMnm), demonstrated that four nm isoforms were present. These four TMnm variants are NM-1, NM-2, NM-3 and NM-4. Nucleotide (nt) sequencing revealed that all these isoforms expressed the nm exon 1b sequence, but varied in their usage of the alternatively spliced exons 6a and b and 9a/b and d, representing skeletal muscle (sk) (exons 6b and 9a/b) and nm (6a and 9d) sequences. A novel exon 9 (designated as exon 9c) was associated with the NM-4 isoform and was also found to be expressed in the cochlea. Comparisons of the nt and amino acid (aa) sequences demonstrated a high homology between rat, mouse and human sequences encoding the 'classical' nm isoform, TM30nm, which is designated herein as NM-1. The rat NM-1 nt homology with mouse and human sequences also included the 3' untranslated region. Homologies were found between aa sequences of the C termini of NM-1 and the TMa smooth muscle and TMβ nm isoforms, between the sk sequence of NM-3 and the TMα and β sk isoforms, and between the novel 9c sequence of NM-4 and the TMα brain-1 isoform. These data predict that the nm isoforms share biochemical properties described for the other TM isoforms.

Original languageEnglish
Pages (from-to)251-256
Number of pages6
JournalGene
Volume143
Issue number2
DOIs
StatePublished - Jun 10 1994

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Tropomyosin
Cochlea
Protein Isoforms
Exons
Genes
Nucleotides
Amino Acid Sequence
3' Untranslated Regions
Gene Library
Smooth Muscle
Skeletal Muscle

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Identification of novel alternatively spliced isoforms of the tropomyosin-encoding gene, TMnm, in the rat cochlea. / Beisel, Kirk; Kennedy, Jane E.

In: Gene, Vol. 143, No. 2, 10.06.1994, p. 251-256.

Research output: Contribution to journalArticle

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abstract = "Analysis of a rat cochlear cDNA library for the expression of the non-muscle (nm) tropomyosin (TM)-encoding gene (TMnm), demonstrated that four nm isoforms were present. These four TMnm variants are NM-1, NM-2, NM-3 and NM-4. Nucleotide (nt) sequencing revealed that all these isoforms expressed the nm exon 1b sequence, but varied in their usage of the alternatively spliced exons 6a and b and 9a/b and d, representing skeletal muscle (sk) (exons 6b and 9a/b) and nm (6a and 9d) sequences. A novel exon 9 (designated as exon 9c) was associated with the NM-4 isoform and was also found to be expressed in the cochlea. Comparisons of the nt and amino acid (aa) sequences demonstrated a high homology between rat, mouse and human sequences encoding the 'classical' nm isoform, TM30nm, which is designated herein as NM-1. The rat NM-1 nt homology with mouse and human sequences also included the 3' untranslated region. Homologies were found between aa sequences of the C termini of NM-1 and the TMa smooth muscle and TMβ nm isoforms, between the sk sequence of NM-3 and the TMα and β sk isoforms, and between the novel 9c sequence of NM-4 and the TMα brain-1 isoform. These data predict that the nm isoforms share biochemical properties described for the other TM isoforms.",
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AB - Analysis of a rat cochlear cDNA library for the expression of the non-muscle (nm) tropomyosin (TM)-encoding gene (TMnm), demonstrated that four nm isoforms were present. These four TMnm variants are NM-1, NM-2, NM-3 and NM-4. Nucleotide (nt) sequencing revealed that all these isoforms expressed the nm exon 1b sequence, but varied in their usage of the alternatively spliced exons 6a and b and 9a/b and d, representing skeletal muscle (sk) (exons 6b and 9a/b) and nm (6a and 9d) sequences. A novel exon 9 (designated as exon 9c) was associated with the NM-4 isoform and was also found to be expressed in the cochlea. Comparisons of the nt and amino acid (aa) sequences demonstrated a high homology between rat, mouse and human sequences encoding the 'classical' nm isoform, TM30nm, which is designated herein as NM-1. The rat NM-1 nt homology with mouse and human sequences also included the 3' untranslated region. Homologies were found between aa sequences of the C termini of NM-1 and the TMa smooth muscle and TMβ nm isoforms, between the sk sequence of NM-3 and the TMα and β sk isoforms, and between the novel 9c sequence of NM-4 and the TMα brain-1 isoform. These data predict that the nm isoforms share biochemical properties described for the other TM isoforms.

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