Abstract
A small-scale full-length library construction approach was developed to facilitate production of a mouse full-length cDNA encyclopedia representing ∼250 enriched, normalized, and/or subtracted cDNA libraries. One library produced using this approach was a subtracted adult mouse inner ear cDNA library (sIEa). The average size of the inserts was ∼2.5 kb, with the majority ranging from 0.5 to 7.0 kb. From this library 22,574 sequence reads were obtained from 15,958 independent clones. Sequencing and chromosomal localization established 5240 clusters, with 1302 clusters being unique and 359 representing new ESTs. Our sIEa library contributed 56.1% of the 7773 nonredundant Unigene clusters associated with the four mouse inner ear libraries in the NCBI dbEST. Based on homologous chromosomal regions between human and mouse, we identified 1018 UniGene clusters associated with the deafness locus critical regions. Of these, 59 clusters were found only in our sIEa library and represented ∼50% of the identified critical regions.
| Original language | English |
|---|---|
| Pages (from-to) | 1012-1023 |
| Number of pages | 12 |
| Journal | Genomics |
| Volume | 83 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2004 |
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All Science Journal Classification (ASJC) codes
- Genetics
Cite this
Identification of unique transcripts from a mouse full-length, subtracted inner ear cDNA library. / Beisel, Kirk; Shiraki, Toshiyuki; Morris, Ken A.; Pompeia, Celine; Kachar, Bechara; Arakawa, Takahiro; Bono, Hidemasa; Kawai, Jun; Hayashizaki, Yoshihide; Carninci, Piero.
In: Genomics, Vol. 83, No. 6, 06.2004, p. 1012-1023.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of unique transcripts from a mouse full-length, subtracted inner ear cDNA library
AU - Beisel, Kirk
AU - Shiraki, Toshiyuki
AU - Morris, Ken A.
AU - Pompeia, Celine
AU - Kachar, Bechara
AU - Arakawa, Takahiro
AU - Bono, Hidemasa
AU - Kawai, Jun
AU - Hayashizaki, Yoshihide
AU - Carninci, Piero
PY - 2004/6
Y1 - 2004/6
N2 - A small-scale full-length library construction approach was developed to facilitate production of a mouse full-length cDNA encyclopedia representing ∼250 enriched, normalized, and/or subtracted cDNA libraries. One library produced using this approach was a subtracted adult mouse inner ear cDNA library (sIEa). The average size of the inserts was ∼2.5 kb, with the majority ranging from 0.5 to 7.0 kb. From this library 22,574 sequence reads were obtained from 15,958 independent clones. Sequencing and chromosomal localization established 5240 clusters, with 1302 clusters being unique and 359 representing new ESTs. Our sIEa library contributed 56.1% of the 7773 nonredundant Unigene clusters associated with the four mouse inner ear libraries in the NCBI dbEST. Based on homologous chromosomal regions between human and mouse, we identified 1018 UniGene clusters associated with the deafness locus critical regions. Of these, 59 clusters were found only in our sIEa library and represented ∼50% of the identified critical regions.
AB - A small-scale full-length library construction approach was developed to facilitate production of a mouse full-length cDNA encyclopedia representing ∼250 enriched, normalized, and/or subtracted cDNA libraries. One library produced using this approach was a subtracted adult mouse inner ear cDNA library (sIEa). The average size of the inserts was ∼2.5 kb, with the majority ranging from 0.5 to 7.0 kb. From this library 22,574 sequence reads were obtained from 15,958 independent clones. Sequencing and chromosomal localization established 5240 clusters, with 1302 clusters being unique and 359 representing new ESTs. Our sIEa library contributed 56.1% of the 7773 nonredundant Unigene clusters associated with the four mouse inner ear libraries in the NCBI dbEST. Based on homologous chromosomal regions between human and mouse, we identified 1018 UniGene clusters associated with the deafness locus critical regions. Of these, 59 clusters were found only in our sIEa library and represented ∼50% of the identified critical regions.
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U2 - 10.1016/j.ygeno.2004.01.006
DO - 10.1016/j.ygeno.2004.01.006
M3 - Article
VL - 83
SP - 1012
EP - 1023
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 6
ER -