Identification of unique transcripts from a mouse full-length, subtracted inner ear cDNA library

Kirk Beisel, Toshiyuki Shiraki, Ken A. Morris, Celine Pompeia, Bechara Kachar, Takahiro Arakawa, Hidemasa Bono, Jun Kawai, Yoshihide Hayashizaki, Piero Carninci

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A small-scale full-length library construction approach was developed to facilitate production of a mouse full-length cDNA encyclopedia representing ∼250 enriched, normalized, and/or subtracted cDNA libraries. One library produced using this approach was a subtracted adult mouse inner ear cDNA library (sIEa). The average size of the inserts was ∼2.5 kb, with the majority ranging from 0.5 to 7.0 kb. From this library 22,574 sequence reads were obtained from 15,958 independent clones. Sequencing and chromosomal localization established 5240 clusters, with 1302 clusters being unique and 359 representing new ESTs. Our sIEa library contributed 56.1% of the 7773 nonredundant Unigene clusters associated with the four mouse inner ear libraries in the NCBI dbEST. Based on homologous chromosomal regions between human and mouse, we identified 1018 UniGene clusters associated with the deafness locus critical regions. Of these, 59 clusters were found only in our sIEa library and represented ∼50% of the identified critical regions.

Original languageEnglish
Pages (from-to)1012-1023
Number of pages12
JournalGenomics
Volume83
Issue number6
DOIs
StatePublished - Jun 2004

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Inner Ear
Gene Library
Libraries
Encyclopedias
Expressed Sequence Tags
Deafness
Complementary DNA
Clone Cells

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Beisel, K., Shiraki, T., Morris, K. A., Pompeia, C., Kachar, B., Arakawa, T., ... Carninci, P. (2004). Identification of unique transcripts from a mouse full-length, subtracted inner ear cDNA library. Genomics, 83(6), 1012-1023. https://doi.org/10.1016/j.ygeno.2004.01.006

Identification of unique transcripts from a mouse full-length, subtracted inner ear cDNA library. / Beisel, Kirk; Shiraki, Toshiyuki; Morris, Ken A.; Pompeia, Celine; Kachar, Bechara; Arakawa, Takahiro; Bono, Hidemasa; Kawai, Jun; Hayashizaki, Yoshihide; Carninci, Piero.

In: Genomics, Vol. 83, No. 6, 06.2004, p. 1012-1023.

Research output: Contribution to journalArticle

Beisel, K, Shiraki, T, Morris, KA, Pompeia, C, Kachar, B, Arakawa, T, Bono, H, Kawai, J, Hayashizaki, Y & Carninci, P 2004, 'Identification of unique transcripts from a mouse full-length, subtracted inner ear cDNA library', Genomics, vol. 83, no. 6, pp. 1012-1023. https://doi.org/10.1016/j.ygeno.2004.01.006
Beisel, Kirk ; Shiraki, Toshiyuki ; Morris, Ken A. ; Pompeia, Celine ; Kachar, Bechara ; Arakawa, Takahiro ; Bono, Hidemasa ; Kawai, Jun ; Hayashizaki, Yoshihide ; Carninci, Piero. / Identification of unique transcripts from a mouse full-length, subtracted inner ear cDNA library. In: Genomics. 2004 ; Vol. 83, No. 6. pp. 1012-1023.
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