IL-1 receptor-type expression in relation to atopy

Kirtibala Gupta, Againdra K. Bewtra

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: IL-1 has 2 receptors, type I (IL-1RI) and type II (IL- 1RII), which have 2 forms each, membrane (m) and soluble (s). When IL-1 binds to mIL-1RI, the active receptor, an inflammatory response is initiated, which does not occur when IL-1 binds to mIL-1RII, the decoy receptor. Both sIL-1RI and sIL-1RII function as IL-l-mopping mechanisms. We hypothesized that the ratio of active (mIL-1RI) to inactive (mIL-1RII, sIL-1RI, and sIL-1RII) receptors is important in determining the amount of inflammation produced in allergic reactions. Objective: Our aim was to compare the concentrations of mIL-1RI and mIL-1RII on cultured PBLs and sIL-1RI, sIL1RII, and IL-1β in sera and supernatants of cultured PBMCs from atopic and nonatopic subjects. Methods: The membrane receptors, soluble receptors, and IL1β concentrations were measured by ELISA with specific mAbs. Results: Although there was no difference in the level of serum IL-1β between the 2 groups, PBMCs from atopic persons spontaneously secreted higher levels of IL-1β than those from nonatopic donors (P <.05). PBLs from atopic subjects compared with those from nonatopic individuals expressed higher mIL-1RI (P <.0001) and mIL-1RII (P <.05). Levels of both the soluble receptors from both serum (P <.0001) and PBMCs (P <.05) of nonatopic donors were higher than those found in atopic donors. Conclusion: This augmentation of mIL-1RI concomitant with a reduction in soluble receptors may be an important contributory factor to the inflammation that occurs with allergen exposure.

Original languageEnglish
Pages (from-to)1100-1107
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Volume103
Issue number6
DOIs
StatePublished - 1999

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Interleukin-1 Receptors
Interleukin-1
Serum
Inflammation
Membranes
Allergens
Hypersensitivity
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Immunology
  • Immunology and Allergy

Cite this

IL-1 receptor-type expression in relation to atopy. / Gupta, Kirtibala; Bewtra, Againdra K.

In: Journal of Allergy and Clinical Immunology, Vol. 103, No. 6, 1999, p. 1100-1107.

Research output: Contribution to journalArticle

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abstract = "Background: IL-1 has 2 receptors, type I (IL-1RI) and type II (IL- 1RII), which have 2 forms each, membrane (m) and soluble (s). When IL-1 binds to mIL-1RI, the active receptor, an inflammatory response is initiated, which does not occur when IL-1 binds to mIL-1RII, the decoy receptor. Both sIL-1RI and sIL-1RII function as IL-l-mopping mechanisms. We hypothesized that the ratio of active (mIL-1RI) to inactive (mIL-1RII, sIL-1RI, and sIL-1RII) receptors is important in determining the amount of inflammation produced in allergic reactions. Objective: Our aim was to compare the concentrations of mIL-1RI and mIL-1RII on cultured PBLs and sIL-1RI, sIL1RII, and IL-1β in sera and supernatants of cultured PBMCs from atopic and nonatopic subjects. Methods: The membrane receptors, soluble receptors, and IL1β concentrations were measured by ELISA with specific mAbs. Results: Although there was no difference in the level of serum IL-1β between the 2 groups, PBMCs from atopic persons spontaneously secreted higher levels of IL-1β than those from nonatopic donors (P <.05). PBLs from atopic subjects compared with those from nonatopic individuals expressed higher mIL-1RI (P <.0001) and mIL-1RII (P <.05). Levels of both the soluble receptors from both serum (P <.0001) and PBMCs (P <.05) of nonatopic donors were higher than those found in atopic donors. Conclusion: This augmentation of mIL-1RI concomitant with a reduction in soluble receptors may be an important contributory factor to the inflammation that occurs with allergen exposure.",
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AB - Background: IL-1 has 2 receptors, type I (IL-1RI) and type II (IL- 1RII), which have 2 forms each, membrane (m) and soluble (s). When IL-1 binds to mIL-1RI, the active receptor, an inflammatory response is initiated, which does not occur when IL-1 binds to mIL-1RII, the decoy receptor. Both sIL-1RI and sIL-1RII function as IL-l-mopping mechanisms. We hypothesized that the ratio of active (mIL-1RI) to inactive (mIL-1RII, sIL-1RI, and sIL-1RII) receptors is important in determining the amount of inflammation produced in allergic reactions. Objective: Our aim was to compare the concentrations of mIL-1RI and mIL-1RII on cultured PBLs and sIL-1RI, sIL1RII, and IL-1β in sera and supernatants of cultured PBMCs from atopic and nonatopic subjects. Methods: The membrane receptors, soluble receptors, and IL1β concentrations were measured by ELISA with specific mAbs. Results: Although there was no difference in the level of serum IL-1β between the 2 groups, PBMCs from atopic persons spontaneously secreted higher levels of IL-1β than those from nonatopic donors (P <.05). PBLs from atopic subjects compared with those from nonatopic individuals expressed higher mIL-1RI (P <.0001) and mIL-1RII (P <.05). Levels of both the soluble receptors from both serum (P <.0001) and PBMCs (P <.05) of nonatopic donors were higher than those found in atopic donors. Conclusion: This augmentation of mIL-1RI concomitant with a reduction in soluble receptors may be an important contributory factor to the inflammation that occurs with allergen exposure.

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