Immediate and ongoing detection of prions in the blood of hamsters and deer following oral, nasal, or blood inoculations

Alan M. Elder; Davin M. Henderson; Amy V. Nalls; Edward A. Hoover; Anthony E. Kincaid; Jason C. Bartz; Candace K. Mathiason

doi:10.1128/JVI.00760-15

Immediate and ongoing detection of prions in the blood of hamsters and deer following oral, nasal, or blood inoculations

Alan M. Elder, Davin M. Henderson, Amy V. Nalls, Edward A. Hoover, Anthony E. Kincaid, Jason C. Bartz, Candace K. Mathiason

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Infectious prions traverse epithelial barriers to gain access to the circulatory system, yet the temporal parameters of transepithelial transport and persistence in the blood over time remain unknown. We used whole-blood real-time quaking-induced conversion (wbRT-QuIC) to analyze whole blood collected from transmissible spongiform encephalopathy (TSE)-inoculated deer and hamsters throughout the incubation period for the presence of common prion protein-conversion competent amyloid (PrP^C-CCA). We observed PrP^C-CCA in the blood of TSE-inoculated hosts throughout the disease course from minutes postexposure to terminal disease.

Original language	English (US)
Pages (from-to)	7421-7424
Number of pages	4
Journal	Journal of Virology
Volume	89
Issue number	14
DOIs	https://doi.org/10.1128/JVI.00760-15
State	Published - 2015

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Insect Science
Virology

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10.1128/JVI.00760-15

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title = "Immediate and ongoing detection of prions in the blood of hamsters and deer following oral, nasal, or blood inoculations",

abstract = "Infectious prions traverse epithelial barriers to gain access to the circulatory system, yet the temporal parameters of transepithelial transport and persistence in the blood over time remain unknown. We used whole-blood real-time quaking-induced conversion (wbRT-QuIC) to analyze whole blood collected from transmissible spongiform encephalopathy (TSE)-inoculated deer and hamsters throughout the incubation period for the presence of common prion protein-conversion competent amyloid (PrPC-CCA). We observed PrPC-CCA in the blood of TSE-inoculated hosts throughout the disease course from minutes postexposure to terminal disease.",

author = "Elder, {Alan M.} and Henderson, {Davin M.} and Nalls, {Amy V.} and Hoover, {Edward A.} and Kincaid, {Anthony E.} and Bartz, {Jason C.} and Mathiason, {Candace K.}",

note = "Publisher Copyright: {\textcopyright} 2015, American Society for Microbiology.",

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T1 - Immediate and ongoing detection of prions in the blood of hamsters and deer following oral, nasal, or blood inoculations

AU - Elder, Alan M.

AU - Henderson, Davin M.

AU - Nalls, Amy V.

AU - Hoover, Edward A.

AU - Kincaid, Anthony E.

AU - Bartz, Jason C.

AU - Mathiason, Candace K.

PY - 2015

Y1 - 2015

N2 - Infectious prions traverse epithelial barriers to gain access to the circulatory system, yet the temporal parameters of transepithelial transport and persistence in the blood over time remain unknown. We used whole-blood real-time quaking-induced conversion (wbRT-QuIC) to analyze whole blood collected from transmissible spongiform encephalopathy (TSE)-inoculated deer and hamsters throughout the incubation period for the presence of common prion protein-conversion competent amyloid (PrPC-CCA). We observed PrPC-CCA in the blood of TSE-inoculated hosts throughout the disease course from minutes postexposure to terminal disease.

AB - Infectious prions traverse epithelial barriers to gain access to the circulatory system, yet the temporal parameters of transepithelial transport and persistence in the blood over time remain unknown. We used whole-blood real-time quaking-induced conversion (wbRT-QuIC) to analyze whole blood collected from transmissible spongiform encephalopathy (TSE)-inoculated deer and hamsters throughout the incubation period for the presence of common prion protein-conversion competent amyloid (PrPC-CCA). We observed PrPC-CCA in the blood of TSE-inoculated hosts throughout the disease course from minutes postexposure to terminal disease.

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Immediate and ongoing detection of prions in the blood of hamsters and deer following oral, nasal, or blood inoculations

Abstract

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