Immortalization of xeroderma pigmentosum cells by simian virus 40 DNA having a defective origin of DNA replication

Dan Canaani, Tova Naiman, Tal Teitz, Paul Berg

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

A simian virus 40 (SV40) DNA fragment, encompassing the whole early region and having a defective origin of DNA replication, has been used to transform human fibroblast cells derived from two xeroderma pigmentosum (XP) patients. Two of the SV40-transformed XP cell lines, belonging to complementation group C, had acquired the characteristic of indefinite life-span in culture. These XP cell lines synthesize T antigen as shown by immunofluorescence and retain the high sensitivity to UV irradiation. Detailed karyotype analysis shows very few chromosomal changes, while the transfecting SV40 DNA is integrated into cellular DNA sequences. These are the first immortalized XP cell lines derived from complementation group C. In view of the extreme difficulty in obtaining immortalized human fibroblasts, we suggest a possible advantage of replication defective SV40 DNA molecules for immortalizing human fibroblast cells of any source.

Original languageEnglish (US)
Pages (from-to)13-20
Number of pages8
JournalSomatic Cell and Molecular Genetics
Volume12
Issue number1
DOIs
StatePublished - Jan 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

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