Impact of Anaerobic Growth Conditions on Toxic Shock Syndrome Toxin-1 Production by Staphylococcus aureus

Max Lungren, Pongsakdi Ghaisilwattana, Floyd C. Knoop, Gilles R.G. Monif

Research output: Contribution to journalArticle

Abstract

Objective: The impact of anaerobic growth conditions on the Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1) production was studied. Methods: Ten strains of S. aureus derived from patients with toxic shock syndrome (TSS), 10 isolates of S. aureus, and documented TSST-1-producing strains recovered from patients with either staphylococcal septicemia or staphylococcal nongenital abscesses were grown under aerobic and anaerobic conditions. The bacterial growth was measured using optical density (OD) determinations at 520 nm. The toxin production was assayed using the TS-RPLA latex agglutination test. Results: Both TSS and non-TSS strains of S. aureus grown under aerobic and anaerobic conditions exhibited comparable OD patterns of growth, and the levels of toxin production remained constant during the logarithmic phase. Toxin titers developed during the logarithmic growth phase and peaked after 24 h of incubation. When stationary-phase isolates grown initially under aerobic conditions were subjected to strict anaerobic conditions, subsequent toxin titers, compared with isolates grown in the continued presence of oxygen, were depressed 2-fold, peaking at a later time. Conclusions: TSST-1 production is diminished under continued anaerobic conditions.

Original languageEnglish (US)
Pages (from-to)232-238
Number of pages7
JournalInfectious Diseases in Obstetrics and Gynecology
Volume4
Issue number4
DOIs
StatePublished - Jan 1 1996

Fingerprint

Staphylococcus aureus
Septic Shock
Growth
Latex Fixation Tests
Abscess
Shock
Sepsis
Oxygen
Staphylococcal enterotoxin F

All Science Journal Classification (ASJC) codes

  • Dermatology
  • Obstetrics and Gynecology
  • Infectious Diseases

Cite this

Impact of Anaerobic Growth Conditions on Toxic Shock Syndrome Toxin-1 Production by Staphylococcus aureus. / Lungren, Max; Ghaisilwattana, Pongsakdi; Knoop, Floyd C.; Monif, Gilles R.G.

In: Infectious Diseases in Obstetrics and Gynecology, Vol. 4, No. 4, 01.01.1996, p. 232-238.

Research output: Contribution to journalArticle

@article{d2fa3a39814c42f3bbc722216eda3df1,
title = "Impact of Anaerobic Growth Conditions on Toxic Shock Syndrome Toxin-1 Production by Staphylococcus aureus",
abstract = "Objective: The impact of anaerobic growth conditions on the Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1) production was studied. Methods: Ten strains of S. aureus derived from patients with toxic shock syndrome (TSS), 10 isolates of S. aureus, and documented TSST-1-producing strains recovered from patients with either staphylococcal septicemia or staphylococcal nongenital abscesses were grown under aerobic and anaerobic conditions. The bacterial growth was measured using optical density (OD) determinations at 520 nm. The toxin production was assayed using the TS-RPLA latex agglutination test. Results: Both TSS and non-TSS strains of S. aureus grown under aerobic and anaerobic conditions exhibited comparable OD patterns of growth, and the levels of toxin production remained constant during the logarithmic phase. Toxin titers developed during the logarithmic growth phase and peaked after 24 h of incubation. When stationary-phase isolates grown initially under aerobic conditions were subjected to strict anaerobic conditions, subsequent toxin titers, compared with isolates grown in the continued presence of oxygen, were depressed 2-fold, peaking at a later time. Conclusions: TSST-1 production is diminished under continued anaerobic conditions.",
author = "Max Lungren and Pongsakdi Ghaisilwattana and Knoop, {Floyd C.} and Monif, {Gilles R.G.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1155/S1064744996000440",
language = "English (US)",
volume = "4",
pages = "232--238",
journal = "Infectious Diseases in Obstetrics and Gynecology",
issn = "1064-7449",
publisher = "Hindawi Publishing Corporation",
number = "4",

}

TY - JOUR

T1 - Impact of Anaerobic Growth Conditions on Toxic Shock Syndrome Toxin-1 Production by Staphylococcus aureus

AU - Lungren, Max

AU - Ghaisilwattana, Pongsakdi

AU - Knoop, Floyd C.

AU - Monif, Gilles R.G.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Objective: The impact of anaerobic growth conditions on the Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1) production was studied. Methods: Ten strains of S. aureus derived from patients with toxic shock syndrome (TSS), 10 isolates of S. aureus, and documented TSST-1-producing strains recovered from patients with either staphylococcal septicemia or staphylococcal nongenital abscesses were grown under aerobic and anaerobic conditions. The bacterial growth was measured using optical density (OD) determinations at 520 nm. The toxin production was assayed using the TS-RPLA latex agglutination test. Results: Both TSS and non-TSS strains of S. aureus grown under aerobic and anaerobic conditions exhibited comparable OD patterns of growth, and the levels of toxin production remained constant during the logarithmic phase. Toxin titers developed during the logarithmic growth phase and peaked after 24 h of incubation. When stationary-phase isolates grown initially under aerobic conditions were subjected to strict anaerobic conditions, subsequent toxin titers, compared with isolates grown in the continued presence of oxygen, were depressed 2-fold, peaking at a later time. Conclusions: TSST-1 production is diminished under continued anaerobic conditions.

AB - Objective: The impact of anaerobic growth conditions on the Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1) production was studied. Methods: Ten strains of S. aureus derived from patients with toxic shock syndrome (TSS), 10 isolates of S. aureus, and documented TSST-1-producing strains recovered from patients with either staphylococcal septicemia or staphylococcal nongenital abscesses were grown under aerobic and anaerobic conditions. The bacterial growth was measured using optical density (OD) determinations at 520 nm. The toxin production was assayed using the TS-RPLA latex agglutination test. Results: Both TSS and non-TSS strains of S. aureus grown under aerobic and anaerobic conditions exhibited comparable OD patterns of growth, and the levels of toxin production remained constant during the logarithmic phase. Toxin titers developed during the logarithmic growth phase and peaked after 24 h of incubation. When stationary-phase isolates grown initially under aerobic conditions were subjected to strict anaerobic conditions, subsequent toxin titers, compared with isolates grown in the continued presence of oxygen, were depressed 2-fold, peaking at a later time. Conclusions: TSST-1 production is diminished under continued anaerobic conditions.

UR - http://www.scopus.com/inward/record.url?scp=84956443528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84956443528&partnerID=8YFLogxK

U2 - 10.1155/S1064744996000440

DO - 10.1155/S1064744996000440

M3 - Article

VL - 4

SP - 232

EP - 238

JO - Infectious Diseases in Obstetrics and Gynecology

JF - Infectious Diseases in Obstetrics and Gynecology

SN - 1064-7449

IS - 4

ER -