Impact of female cigarette smoking on circulating B cells in vivo

The suppressed ICOSLG, TCF3, and VCAM1 gene functional network may inhibit normal cell function

Feng Pan, Tie Lin Yang, Xiang Ding Chen, Yuan Chen, Ge Gao, Yao Zhong Liu, Yu Fang Pei, Bao Yong Sha, Yan Jiang, Chao Xu, Robert R. Recker, Hong Wen Deng

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

As pivotal immune guardians, B cells were found to be directly associated with the onset and development of many smoking-induced diseases. However, the in vivo molecular response of B cells underlying the female cigarette smoking remains unknown. Using the genome-wide Affymetrix HG-133A GeneChip® microarray, we firstly compared the gene expression profiles of peripheral circulating B cells between 39 smoking and 40 non-smoking healthy US white women. A total of 125 differential expressed genes were identified in our study, and 75.2% of them were down-regulated in smokers. We further obtained genotypes of 702 single nucleotide polymorphisms in those promising genes and assessed their associations with smoking status. Using a novel multicriteria evaluation model integrating information from microarray and the association studies, several genes were further revealed to play important roles in the response of smoking, including ICOSLG (CD275, inducible T-cell co-stimulator ligand), TCF3 (E2A immunoglobulin enhancer binding factors E12/E47), VCAM1 (CD106, vascular cell adhesion molecule 1), CCR1 (CD191, chemokine C-C motif receptor 1) and IL13 (interleukin 13). The differential expression of ICOSLG (p∈=∈0.0130) and TCF3 (p∈=∈0.0125) genes between the two groups were confirmed by real-time reverse transcription PCR experiment. Our findings support the functional importance of the identified genes in response to the smoking stimulus. This is the first in vivo genome-wide expression study on B cells at today's context of high prevalence rate of smoking for women. Our results highlight the potential usage of integrated analyses for unveiling the novel pathogenesis mechanism and emphasized the significance of B cells in the etiology of smoking-induced disease.

Original languageEnglish
Pages (from-to)237-251
Number of pages15
JournalImmunogenetics
Volume62
Issue number4
DOIs
StatePublished - Apr 2010

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Inducible T-Cell Co-Stimulator Ligand
Vascular Cell Adhesion Molecule-1
Gene Regulatory Networks
B-Lymphocytes
Smoking
Transcription Factor 3
Genes
CCR Receptors
Genome
Interleukin-13
Transcriptome
Reverse Transcription
Single Nucleotide Polymorphism

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Impact of female cigarette smoking on circulating B cells in vivo : The suppressed ICOSLG, TCF3, and VCAM1 gene functional network may inhibit normal cell function. / Pan, Feng; Yang, Tie Lin; Chen, Xiang Ding; Chen, Yuan; Gao, Ge; Liu, Yao Zhong; Pei, Yu Fang; Sha, Bao Yong; Jiang, Yan; Xu, Chao; Recker, Robert R.; Deng, Hong Wen.

In: Immunogenetics, Vol. 62, No. 4, 04.2010, p. 237-251.

Research output: Contribution to journalArticle

Pan, Feng ; Yang, Tie Lin ; Chen, Xiang Ding ; Chen, Yuan ; Gao, Ge ; Liu, Yao Zhong ; Pei, Yu Fang ; Sha, Bao Yong ; Jiang, Yan ; Xu, Chao ; Recker, Robert R. ; Deng, Hong Wen. / Impact of female cigarette smoking on circulating B cells in vivo : The suppressed ICOSLG, TCF3, and VCAM1 gene functional network may inhibit normal cell function. In: Immunogenetics. 2010 ; Vol. 62, No. 4. pp. 237-251.
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abstract = "As pivotal immune guardians, B cells were found to be directly associated with the onset and development of many smoking-induced diseases. However, the in vivo molecular response of B cells underlying the female cigarette smoking remains unknown. Using the genome-wide Affymetrix HG-133A GeneChip{\circledR} microarray, we firstly compared the gene expression profiles of peripheral circulating B cells between 39 smoking and 40 non-smoking healthy US white women. A total of 125 differential expressed genes were identified in our study, and 75.2{\%} of them were down-regulated in smokers. We further obtained genotypes of 702 single nucleotide polymorphisms in those promising genes and assessed their associations with smoking status. Using a novel multicriteria evaluation model integrating information from microarray and the association studies, several genes were further revealed to play important roles in the response of smoking, including ICOSLG (CD275, inducible T-cell co-stimulator ligand), TCF3 (E2A immunoglobulin enhancer binding factors E12/E47), VCAM1 (CD106, vascular cell adhesion molecule 1), CCR1 (CD191, chemokine C-C motif receptor 1) and IL13 (interleukin 13). The differential expression of ICOSLG (p∈=∈0.0130) and TCF3 (p∈=∈0.0125) genes between the two groups were confirmed by real-time reverse transcription PCR experiment. Our findings support the functional importance of the identified genes in response to the smoking stimulus. This is the first in vivo genome-wide expression study on B cells at today's context of high prevalence rate of smoking for women. Our results highlight the potential usage of integrated analyses for unveiling the novel pathogenesis mechanism and emphasized the significance of B cells in the etiology of smoking-induced disease.",
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AU - Liu, Yao Zhong

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AU - Sha, Bao Yong

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AU - Xu, Chao

AU - Recker, Robert R.

AU - Deng, Hong Wen

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