In the Telencephalon, GluN2C NMDA Receptor Subunit mRNA is Predominately Expressed in Glial Cells and GluN2D mRNA in Interneurons

Hassan A. Alsaad, Nicholas W. DeKorver, Zhihao Mao, Shashank M. Dravid, Jyothi Arikkath, Daniel T. Monaghan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

N-methyl-d-aspartate receptors (NMDARs) are widely distributed in the brain with high concentrations in the telencephalon where they modulate synaptic plasticity, working memory, and other functions. While the actions of the predominate GluN2 NMDAR subunits, GluN2A and GluN2B are relatively well understood, the function of GluN2C and GluN2D subunits in the telencephalon is largely unknown. To better understand the possible role of GluN2C subunits, we used fluorescence in situ hybridization (FISH) together with multiple cell markers to define the distribution and type of cells expressing GluN2C mRNA. Using a GluN2C-KO mouse as a negative control, GluN2C mRNA expression was only found in non-neuronal cells (NeuN-negative cells) in the hippocampus, striatum, amygdala, and cerebral cortex. For these regions, a significant fraction of GFAP-positive cells also expressed GluN2C mRNA. Overall, for the telencephalon, the globus pallidus and olfactory bulb were the only regions where GluN2C was expressed in neurons. In contrast to GluN2C, GluN2D subunit mRNA colocalized with neuronal and not astrocyte markers or GluN2C mRNA in the telencephalon (except for the globus pallidus). GluN2C mRNA did, however, colocalize with GluN2D in the thalamus where neuronal GluN2C expression is found. These findings strongly suggest that GluN2C has a very distinct function in the telencephalon compared to its role in other brain regions and compared to other GluN2-containing NMDARs. NMDARs containing GluN2C may have a specific role in regulating l-glutamate or d-serine release from astrocytes in response to l-glutamate spillover from synaptic activity.

Original languageEnglish (US)
Pages (from-to)61-77
Number of pages17
JournalNeurochemical Research
Volume44
Issue number1
DOIs
StatePublished - Jan 15 2019

Fingerprint

Telencephalon
Interneurons
N-Methyl-D-Aspartate Receptors
Neuroglia
Messenger RNA
Globus Pallidus
Astrocytes
Glutamic Acid
Brain
Cells
Neuronal Plasticity
Olfactory Bulb
Amygdala
Fluorescence In Situ Hybridization
Thalamus
Short-Term Memory
Cerebral Cortex
Serine
Neurons
Plasticity

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

In the Telencephalon, GluN2C NMDA Receptor Subunit mRNA is Predominately Expressed in Glial Cells and GluN2D mRNA in Interneurons. / Alsaad, Hassan A.; DeKorver, Nicholas W.; Mao, Zhihao; Dravid, Shashank M.; Arikkath, Jyothi; Monaghan, Daniel T.

In: Neurochemical Research, Vol. 44, No. 1, 15.01.2019, p. 61-77.

Research output: Contribution to journalArticle

Alsaad, Hassan A. ; DeKorver, Nicholas W. ; Mao, Zhihao ; Dravid, Shashank M. ; Arikkath, Jyothi ; Monaghan, Daniel T. / In the Telencephalon, GluN2C NMDA Receptor Subunit mRNA is Predominately Expressed in Glial Cells and GluN2D mRNA in Interneurons. In: Neurochemical Research. 2019 ; Vol. 44, No. 1. pp. 61-77.
@article{062408f246464a4881e5d10d6ed01c0f,
title = "In the Telencephalon, GluN2C NMDA Receptor Subunit mRNA is Predominately Expressed in Glial Cells and GluN2D mRNA in Interneurons",
abstract = "N-methyl-d-aspartate receptors (NMDARs) are widely distributed in the brain with high concentrations in the telencephalon where they modulate synaptic plasticity, working memory, and other functions. While the actions of the predominate GluN2 NMDAR subunits, GluN2A and GluN2B are relatively well understood, the function of GluN2C and GluN2D subunits in the telencephalon is largely unknown. To better understand the possible role of GluN2C subunits, we used fluorescence in situ hybridization (FISH) together with multiple cell markers to define the distribution and type of cells expressing GluN2C mRNA. Using a GluN2C-KO mouse as a negative control, GluN2C mRNA expression was only found in non-neuronal cells (NeuN-negative cells) in the hippocampus, striatum, amygdala, and cerebral cortex. For these regions, a significant fraction of GFAP-positive cells also expressed GluN2C mRNA. Overall, for the telencephalon, the globus pallidus and olfactory bulb were the only regions where GluN2C was expressed in neurons. In contrast to GluN2C, GluN2D subunit mRNA colocalized with neuronal and not astrocyte markers or GluN2C mRNA in the telencephalon (except for the globus pallidus). GluN2C mRNA did, however, colocalize with GluN2D in the thalamus where neuronal GluN2C expression is found. These findings strongly suggest that GluN2C has a very distinct function in the telencephalon compared to its role in other brain regions and compared to other GluN2-containing NMDARs. NMDARs containing GluN2C may have a specific role in regulating l-glutamate or d-serine release from astrocytes in response to l-glutamate spillover from synaptic activity.",
author = "Alsaad, {Hassan A.} and DeKorver, {Nicholas W.} and Zhihao Mao and Dravid, {Shashank M.} and Jyothi Arikkath and Monaghan, {Daniel T.}",
year = "2019",
month = "1",
day = "15",
doi = "10.1007/s11064-018-2526-7",
language = "English (US)",
volume = "44",
pages = "61--77",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - In the Telencephalon, GluN2C NMDA Receptor Subunit mRNA is Predominately Expressed in Glial Cells and GluN2D mRNA in Interneurons

AU - Alsaad, Hassan A.

AU - DeKorver, Nicholas W.

AU - Mao, Zhihao

AU - Dravid, Shashank M.

AU - Arikkath, Jyothi

AU - Monaghan, Daniel T.

PY - 2019/1/15

Y1 - 2019/1/15

N2 - N-methyl-d-aspartate receptors (NMDARs) are widely distributed in the brain with high concentrations in the telencephalon where they modulate synaptic plasticity, working memory, and other functions. While the actions of the predominate GluN2 NMDAR subunits, GluN2A and GluN2B are relatively well understood, the function of GluN2C and GluN2D subunits in the telencephalon is largely unknown. To better understand the possible role of GluN2C subunits, we used fluorescence in situ hybridization (FISH) together with multiple cell markers to define the distribution and type of cells expressing GluN2C mRNA. Using a GluN2C-KO mouse as a negative control, GluN2C mRNA expression was only found in non-neuronal cells (NeuN-negative cells) in the hippocampus, striatum, amygdala, and cerebral cortex. For these regions, a significant fraction of GFAP-positive cells also expressed GluN2C mRNA. Overall, for the telencephalon, the globus pallidus and olfactory bulb were the only regions where GluN2C was expressed in neurons. In contrast to GluN2C, GluN2D subunit mRNA colocalized with neuronal and not astrocyte markers or GluN2C mRNA in the telencephalon (except for the globus pallidus). GluN2C mRNA did, however, colocalize with GluN2D in the thalamus where neuronal GluN2C expression is found. These findings strongly suggest that GluN2C has a very distinct function in the telencephalon compared to its role in other brain regions and compared to other GluN2-containing NMDARs. NMDARs containing GluN2C may have a specific role in regulating l-glutamate or d-serine release from astrocytes in response to l-glutamate spillover from synaptic activity.

AB - N-methyl-d-aspartate receptors (NMDARs) are widely distributed in the brain with high concentrations in the telencephalon where they modulate synaptic plasticity, working memory, and other functions. While the actions of the predominate GluN2 NMDAR subunits, GluN2A and GluN2B are relatively well understood, the function of GluN2C and GluN2D subunits in the telencephalon is largely unknown. To better understand the possible role of GluN2C subunits, we used fluorescence in situ hybridization (FISH) together with multiple cell markers to define the distribution and type of cells expressing GluN2C mRNA. Using a GluN2C-KO mouse as a negative control, GluN2C mRNA expression was only found in non-neuronal cells (NeuN-negative cells) in the hippocampus, striatum, amygdala, and cerebral cortex. For these regions, a significant fraction of GFAP-positive cells also expressed GluN2C mRNA. Overall, for the telencephalon, the globus pallidus and olfactory bulb were the only regions where GluN2C was expressed in neurons. In contrast to GluN2C, GluN2D subunit mRNA colocalized with neuronal and not astrocyte markers or GluN2C mRNA in the telencephalon (except for the globus pallidus). GluN2C mRNA did, however, colocalize with GluN2D in the thalamus where neuronal GluN2C expression is found. These findings strongly suggest that GluN2C has a very distinct function in the telencephalon compared to its role in other brain regions and compared to other GluN2-containing NMDARs. NMDARs containing GluN2C may have a specific role in regulating l-glutamate or d-serine release from astrocytes in response to l-glutamate spillover from synaptic activity.

UR - http://www.scopus.com/inward/record.url?scp=85045240689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045240689&partnerID=8YFLogxK

U2 - 10.1007/s11064-018-2526-7

DO - 10.1007/s11064-018-2526-7

M3 - Article

VL - 44

SP - 61

EP - 77

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 1

ER -