In vitro detection of prionemia in TSE-infected cervids and hamsters

Alan M. Elder, Davin M. Henderson, Amy V. Nalls, Jason M. Wilham, Byron W. Caughey, Edward A. Hoover, Anthony Kincaid, Jason C. Bartz, Candace K. Mathiason

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Blood-borne transmission of infectious prions during the symptomatic and asymptomatic stages of disease occurs for both human and animal transmissible spongiform encephalopathies (TSEs). The geographical distribution of the cervid TSE, chronic wasting disease (CWD), continues to spread across North America and the prospective number of individuals harboring an asymptomatic infection of human variant Creutzfeldt-Jakob Disease (vCJD) in the United Kingdom has been projected to be ∼1 in 3000 residents. Thus, it is important to monitor cervid and human blood products to ensure herd health and human safety. Current methods for detecting blood-associated prions rely primarily upon bioassay in laboratory animals. While bioassay provides high sensitivity and specificity, it requires many months, animals, and it is costly. Here we report modification of the real time quaking-induced conversion (RT-QuIC) assay to detect blood-borne prions in whole blood from prion-infected preclinical white-tailed deer, muntjac deer, and Syrian hamsters, attaining sensitivity of >90% while maintaining 100% specificity. Our results indicate that RT-QuIC methodology as modified can provide consistent and reliable detection of blood-borne prions in preclinical and symptomatic stages of two animal TSEs, offering promise for prionemia detection in other species, including humans.

Original languageEnglish
Article numbere80203
JournalPLoS One
Volume8
Issue number11
DOIs
StatePublished - Nov 2013

Fingerprint

Prion Diseases
prion diseases
hamsters
Cricetinae
Prions
prions
Blood
blood
Animals
Deer
Bioassay
Biological Assay
Muntjacs
Chronic Wasting Disease
bioassays
Muntiacus
Creutzfeldt-Jakob Syndrome
chronic wasting disease
Geographical distribution
Asymptomatic Diseases

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Elder, A. M., Henderson, D. M., Nalls, A. V., Wilham, J. M., Caughey, B. W., Hoover, E. A., ... Mathiason, C. K. (2013). In vitro detection of prionemia in TSE-infected cervids and hamsters. PLoS One, 8(11), [e80203]. https://doi.org/10.1371/journal.pone.0080203

In vitro detection of prionemia in TSE-infected cervids and hamsters. / Elder, Alan M.; Henderson, Davin M.; Nalls, Amy V.; Wilham, Jason M.; Caughey, Byron W.; Hoover, Edward A.; Kincaid, Anthony; Bartz, Jason C.; Mathiason, Candace K.

In: PLoS One, Vol. 8, No. 11, e80203, 11.2013.

Research output: Contribution to journalArticle

Elder, AM, Henderson, DM, Nalls, AV, Wilham, JM, Caughey, BW, Hoover, EA, Kincaid, A, Bartz, JC & Mathiason, CK 2013, 'In vitro detection of prionemia in TSE-infected cervids and hamsters', PLoS One, vol. 8, no. 11, e80203. https://doi.org/10.1371/journal.pone.0080203
Elder AM, Henderson DM, Nalls AV, Wilham JM, Caughey BW, Hoover EA et al. In vitro detection of prionemia in TSE-infected cervids and hamsters. PLoS One. 2013 Nov;8(11). e80203. https://doi.org/10.1371/journal.pone.0080203
Elder, Alan M. ; Henderson, Davin M. ; Nalls, Amy V. ; Wilham, Jason M. ; Caughey, Byron W. ; Hoover, Edward A. ; Kincaid, Anthony ; Bartz, Jason C. ; Mathiason, Candace K. / In vitro detection of prionemia in TSE-infected cervids and hamsters. In: PLoS One. 2013 ; Vol. 8, No. 11.
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