In vitro differentiation of bone marrow derived porcine mesenchymal stem cells to endothelial cells

Divya Pankajakshan, Vikash Kansal, Devendra K. Agrawal

Research output: Contribution to journalArticle

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Abstract

Mesenchymal stem cells (MSCs) hold potential for the regeneration of damaged tissues in cardiovascular diseases. In this study, we investigated the potential of porcine MSCs to differentiate into endothelial cells (ECs) in vitro. The cultured bone marrow-derived cells were CD11b-CD34-CD44+CD45-CD90+ and showed mesodermal lineage differentiation, which is characteristic of MSCs. The MSCs were induced to differentiate into ECs using endothelial growth medium (EGM), with and without high concentrations of VEGF (EGM+VEGF; 50 ng/ml). Endothelial basal medium (EBM) without growth factors served as the control. The EC differentiation was assessed by the presence of vWF, ability to take up acetylated LDL, in vitro angiogenesis assay, flow cytometry and qPCR of EC markers vWF, VE-cadherin, PECAM-1, VEGF-R1 and VEGF-R2 after 10 days of stimulation. Cells cultured in EGM+VEGF medium demonstrated higher amounts of DiI-AcLDL-positive cells and enhanced the presence of vWF (90%), VE-Cadherin- (60%) and PECAM-1 (48%)-positive cells, than in EBM. These cells showed profuse sprouting of capillary tubes and closed polygon formation in the angiogenesis assay. There was 1.5-2-fold increase in the mRNA expression of endothelial markers in the cells stimulated with EGM+VEGF medium when compared to control. The results demonstrate the ability of porcine MSCs to differentiate into ECs under in vitro inducing conditions. The differentiated cells would provide new options for re-endothelialization following interventional procedures and tissue engineering.

Original languageEnglish
Pages (from-to)911-920
Number of pages10
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume7
Issue number11
DOIs
StatePublished - Nov 2013

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Endothelial cells
Stem cells
Mesenchymal Stromal Cells
Vascular Endothelial Growth Factor A
Bone
Swine
Endothelial Cells
Bone Marrow
CD31 Antigens
Growth
Assays
Cells
Cultured Cells
Capillary tubes
Flow cytometry
Tissue engineering
Cadherins
Tissue Engineering
Bone Marrow Cells
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

In vitro differentiation of bone marrow derived porcine mesenchymal stem cells to endothelial cells. / Pankajakshan, Divya; Kansal, Vikash; Agrawal, Devendra K.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 7, No. 11, 11.2013, p. 911-920.

Research output: Contribution to journalArticle

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