Abstract
The kinetic study of the incorporation of exogenous ganglioside GM3 into the lipid bilayer of rabbit sarcoplasmic reticulum (SR) was carried out by trace extraction of Gls and high performance TLC. The results showed that the amount of incorporation is related to GM3 concentration, incubation temperature and time. The optimum incorporation was observed when 8 μmol/L GM3 was incubated with SR vesicles for 90 min at 35°C. The obtained results suggested that the effect of exogenous ganglioside GM3 on SR membrane is not only a simple aqueous phase reaction, but also a kinetic course of the incorporation into the lipid bilayer of SR. Furthermore, the incorporation of GM3 can markedly increase the activity of SR Ca2+-ATPase. This provides basic experimental data to study the role of gangliosides in intracellular membranes.
| Original language | English |
|---|---|
| Pages (from-to) | 277-280 |
| Number of pages | 4 |
| Journal | Progress in Biochemistry and Biophysics |
| Volume | 24 |
| Issue number | 3 |
| State | Published - 1997 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Biophysics
Cite this
Incorporation of GM3 into the lipid bilayer of sarcoplasmic reticulum and its regulation on Ca2+-ATPase. / Wang, Lihua; Yang, Xiaoyi; Tu, Yaping; Cui, Zhaochun.
In: Progress in Biochemistry and Biophysics, Vol. 24, No. 3, 1997, p. 277-280.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Incorporation of GM3 into the lipid bilayer of sarcoplasmic reticulum and its regulation on Ca2+-ATPase
AU - Wang, Lihua
AU - Yang, Xiaoyi
AU - Tu, Yaping
AU - Cui, Zhaochun
PY - 1997
Y1 - 1997
N2 - The kinetic study of the incorporation of exogenous ganglioside GM3 into the lipid bilayer of rabbit sarcoplasmic reticulum (SR) was carried out by trace extraction of Gls and high performance TLC. The results showed that the amount of incorporation is related to GM3 concentration, incubation temperature and time. The optimum incorporation was observed when 8 μmol/L GM3 was incubated with SR vesicles for 90 min at 35°C. The obtained results suggested that the effect of exogenous ganglioside GM3 on SR membrane is not only a simple aqueous phase reaction, but also a kinetic course of the incorporation into the lipid bilayer of SR. Furthermore, the incorporation of GM3 can markedly increase the activity of SR Ca2+-ATPase. This provides basic experimental data to study the role of gangliosides in intracellular membranes.
AB - The kinetic study of the incorporation of exogenous ganglioside GM3 into the lipid bilayer of rabbit sarcoplasmic reticulum (SR) was carried out by trace extraction of Gls and high performance TLC. The results showed that the amount of incorporation is related to GM3 concentration, incubation temperature and time. The optimum incorporation was observed when 8 μmol/L GM3 was incubated with SR vesicles for 90 min at 35°C. The obtained results suggested that the effect of exogenous ganglioside GM3 on SR membrane is not only a simple aqueous phase reaction, but also a kinetic course of the incorporation into the lipid bilayer of SR. Furthermore, the incorporation of GM3 can markedly increase the activity of SR Ca2+-ATPase. This provides basic experimental data to study the role of gangliosides in intracellular membranes.
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UR - http://www.scopus.com/inward/citedby.url?scp=23744481936&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:23744481936
VL - 24
SP - 277
EP - 280
JO - Progress in Biochemistry and Biophysics
JF - Progress in Biochemistry and Biophysics
SN - 1000-3282
IS - 3
ER -