Increased expression of ampC in Pseudomonas aeruginosa mutants selected with ciprofloxacin

Daniel J. Wolter, Amber J. Schmidtke, Nancy D. Hanson, Philip D. Lister

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Two Pseudomonas aeruginosa mutants exhibiting increased expression of ampC were selected during exposure to ciprofloxacin. These mutants also exhibited significant increases in mexCD-oprJ expression, but further studies failed to show a link between the increased expression of mexCD-oprJ and ampC. Increased ampC expression was not related to mutations within ampR, the ampC-ampR intergenic region, ampD, ampDh2, or ampDh3 or to changes in the levels of expression of these amidase genes. However, ampD complementation restored wild-type levels of ampC expression and ceftazidime susceptibility, suggesting alternative mechanisms of ampC regulation.

Original languageEnglish
Pages (from-to)2997-3000
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume51
Issue number8
DOIs
StatePublished - Aug 2007

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amidase
Intergenic DNA
Ceftazidime
Ciprofloxacin
Pseudomonas aeruginosa
Gene Expression
Mutation

All Science Journal Classification (ASJC) codes

  • Pharmacology (medical)

Cite this

Increased expression of ampC in Pseudomonas aeruginosa mutants selected with ciprofloxacin. / Wolter, Daniel J.; Schmidtke, Amber J.; Hanson, Nancy D.; Lister, Philip D.

In: Antimicrobial Agents and Chemotherapy, Vol. 51, No. 8, 08.2007, p. 2997-3000.

Research output: Contribution to journalArticle

Wolter, Daniel J. ; Schmidtke, Amber J. ; Hanson, Nancy D. ; Lister, Philip D. / Increased expression of ampC in Pseudomonas aeruginosa mutants selected with ciprofloxacin. In: Antimicrobial Agents and Chemotherapy. 2007 ; Vol. 51, No. 8. pp. 2997-3000.
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