Increased expression of triggering receptor expressed on myeloid cells-1 in the population with obesity and insulin resistance

Saravanan Subramanian, Pradeep K. Pallati, Vikrant Rai, Poonam K Sharma, Devendra K. Agrawal, Kalyana C. Nandipati

12 Scopus citations

Abstract

Objective: Triggering receptor expressed on myeloid cells (TREM)−1 has recently been recognized as one of the potent amplifiers of acute and chronic inflammation. However, the exact role of TREM-1 in regard to insulin insensitivity is unknown. Methods: mRNA transcripts and protein expression of TREM-1, TREM-2, and TREM-1/TREM-2 ratio were examined in the tissue biopsies (liver, omentum, and subcutaneous fat) and blood samples (neutrophils and monocytes) of subjects with obesity and diabetes (SO+ D+; n = 15), subjects with obesity but not diabetes (SO+ D; n = 7), and subjects without obesity (BMI < 30) and diabetes (SOD; n = 5). Results: The immunofluorescence and RT-PCR revealed significant increase in TREM-1, decrease in TREM-2, and increase in the TREM1/TREM2 ratio in SO+ D+ group compared with other groups. Overall, increased liver TREM-1 expression and soluble-TREM-1 were found in SO+ D+ group compared with SO+ D group (100% vs. 57.14%, r = 0.582; P = 0.023). TREM-1 was significantly increased in all subjects with obesity and those with HOMA-IR index of >2. Conclusions: TREM-1 was found to be significantly higher in tissues biopsies and blood of subjects with obesity. Greater expression and activity of TREM-1 suggest a possible role in the underlying pathophysiology of obesity and associated comorbidities.

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Increased expression of triggering receptor expressed on myeloid cells-1 in the population with obesity and insulin resistance. / Subramanian, Saravanan; Pallati, Pradeep K.; Rai, Vikrant; Sharma, Poonam K; Agrawal, Devendra K.; Nandipati, Kalyana C.

In: Obesity, Vol. 25, No. 3, 01.03.2017, p. 527-538.

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