Increased hypodense eosinophils after activation with PAF-acether and calcium ionophore in asthmatic subjects

Devendra K. Agrawal, Emmanuel U. Sarmiento, Makoto Nabe, Hidefumi Miyagawa, Robert G. Townley

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Eosinophils play a major role in the pathogenesis of bronchial asthma. In this study, we examined the density characteristics of blood eosinophils from 9 normal healthy individuals and 9 allergic asthmatic patients. Furthermore, the effect of platelet-activating factor, a potent mediator of inflammation, and calcium ionophore, A23187, on the density of normodense eosinophils (density > 1.085 g/ml) has also been examined. Initially, asthmatic patients had 27.0 ± 1.1% eosinophils of lighter density (density ≤ 1.081 g/ml), significantly greater than that in the normal individuals (7.5 ± 0.5%). After exposure to platelet-activating factor (1 μM) or calcium ionophore (A23187, 1 μg/ml), the normodense eosinophils switched to hypodense in both groups: 16.7 ± 2.1% and 54.2 ± 3.7%, respectively, in normal individuals, and 30.6 ± 5.7% and 77.4 ± 2.3%, respectively, in asthmatic patients. These data demonstrated that a certain percentage of normodense eosinophils from asthmatics and normal subjects switched to hypodense after activation with platelet-activating factor or calcium ionophore. Furthermore; eosinophils from asthmatics switched to a greater degree than in normal subjects, suggesting that the normodense eosinophils in asthmatics become primed probably by endogenously released mediators.

Original languageEnglish
Pages (from-to)213-219
Number of pages7
JournalJournal of Asthma
Volume33
Issue number4
StatePublished - 1996

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Calcium Ionophores
Platelet Activating Factor
Eosinophils
Calcimycin
Inflammation Mediators
Asthma

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

Increased hypodense eosinophils after activation with PAF-acether and calcium ionophore in asthmatic subjects. / Agrawal, Devendra K.; Sarmiento, Emmanuel U.; Nabe, Makoto; Miyagawa, Hidefumi; Townley, Robert G.

In: Journal of Asthma, Vol. 33, No. 4, 1996, p. 213-219.

Research output: Contribution to journalArticle

Agrawal, DK, Sarmiento, EU, Nabe, M, Miyagawa, H & Townley, RG 1996, 'Increased hypodense eosinophils after activation with PAF-acether and calcium ionophore in asthmatic subjects', Journal of Asthma, vol. 33, no. 4, pp. 213-219.
Agrawal, Devendra K. ; Sarmiento, Emmanuel U. ; Nabe, Makoto ; Miyagawa, Hidefumi ; Townley, Robert G. / Increased hypodense eosinophils after activation with PAF-acether and calcium ionophore in asthmatic subjects. In: Journal of Asthma. 1996 ; Vol. 33, No. 4. pp. 213-219.
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