Increased hypodense eosinophils after activation with PAF-acether and calcium ionophore in asthmatic subjects

Devendra K. Agrawal, Emmanuel U. Sarmiento, Makoto Nabe, Hidefumi Miyagawa, Robert G. Townley

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Eosinophils play a major role in the pathogenesis of bronchial asthma. In this study, we examined the density characteristics of blood eosinophils from 9 normal healthy individuals and 9 allergic asthmatic patients. Furthermore, the effect of platelet-activating factor, a potent mediator of inflammation, and calcium ionophore, A23187, on the density of normodense eosinophils (density > 1.085 g/ml) has also been examined. Initially, asthmatic patients had 27.0 ± 1.1% eosinophils of lighter density (density ≤ 1.081 g/ml), significantly greater than that in the normal individuals (7.5 ± 0.5%). After exposure to platelet-activating factor (1 μM) or calcium ionophore (A23187, 1 μg/ml), the normodense eosinophils switched to hypodense in both groups: 16.7 ± 2.1% and 54.2 ± 3.7%, respectively, in normal individuals, and 30.6 ± 5.7% and 77.4 ± 2.3%, respectively, in asthmatic patients. These data demonstrated that a certain percentage of normodense eosinophils from asthmatics and normal subjects switched to hypodense after activation with platelet-activating factor or calcium ionophore. Furthermore; eosinophils from asthmatics switched to a greater degree than in normal subjects, suggesting that the normodense eosinophils in asthmatics become primed probably by endogenously released mediators.

Original languageEnglish (US)
Pages (from-to)213-219
Number of pages7
JournalJournal of Asthma
Volume33
Issue number4
DOIs
StatePublished - Jan 1 1996

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Immunology and Allergy
  • Pulmonary and Respiratory Medicine

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