Abstract
Eosinophils play a major role in the pathogenesis of bronchial asthma. In this study, we examined the density characteristics of blood eosinophils from 9 normal healthy individuals and 9 allergic asthmatic patients. Furthermore, the effect of platelet-activating factor, a potent mediator of inflammation, and calcium ionophore, A23187, on the density of normodense eosinophils (density > 1.085 g/ml) has also been examined. Initially, asthmatic patients had 27.0 ± 1.1% eosinophils of lighter density (density ≤ 1.081 g/ml), significantly greater than that in the normal individuals (7.5 ± 0.5%). After exposure to platelet-activating factor (1 μM) or calcium ionophore (A23187, 1 μg/ml), the normodense eosinophils switched to hypodense in both groups: 16.7 ± 2.1% and 54.2 ± 3.7%, respectively, in normal individuals, and 30.6 ± 5.7% and 77.4 ± 2.3%, respectively, in asthmatic patients. These data demonstrated that a certain percentage of normodense eosinophils from asthmatics and normal subjects switched to hypodense after activation with platelet-activating factor or calcium ionophore. Furthermore; eosinophils from asthmatics switched to a greater degree than in normal subjects, suggesting that the normodense eosinophils in asthmatics become primed probably by endogenously released mediators.
| Original language | English |
|---|---|
| Pages (from-to) | 213-219 |
| Number of pages | 7 |
| Journal | Journal of Asthma |
| Volume | 33 |
| Issue number | 4 |
| State | Published - 1996 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Pulmonary and Respiratory Medicine
Cite this
Increased hypodense eosinophils after activation with PAF-acether and calcium ionophore in asthmatic subjects. / Agrawal, Devendra K.; Sarmiento, Emmanuel U.; Nabe, Makoto; Miyagawa, Hidefumi; Townley, Robert G.
In: Journal of Asthma, Vol. 33, No. 4, 1996, p. 213-219.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Increased hypodense eosinophils after activation with PAF-acether and calcium ionophore in asthmatic subjects
AU - Agrawal, Devendra K.
AU - Sarmiento, Emmanuel U.
AU - Nabe, Makoto
AU - Miyagawa, Hidefumi
AU - Townley, Robert G.
PY - 1996
Y1 - 1996
N2 - Eosinophils play a major role in the pathogenesis of bronchial asthma. In this study, we examined the density characteristics of blood eosinophils from 9 normal healthy individuals and 9 allergic asthmatic patients. Furthermore, the effect of platelet-activating factor, a potent mediator of inflammation, and calcium ionophore, A23187, on the density of normodense eosinophils (density > 1.085 g/ml) has also been examined. Initially, asthmatic patients had 27.0 ± 1.1% eosinophils of lighter density (density ≤ 1.081 g/ml), significantly greater than that in the normal individuals (7.5 ± 0.5%). After exposure to platelet-activating factor (1 μM) or calcium ionophore (A23187, 1 μg/ml), the normodense eosinophils switched to hypodense in both groups: 16.7 ± 2.1% and 54.2 ± 3.7%, respectively, in normal individuals, and 30.6 ± 5.7% and 77.4 ± 2.3%, respectively, in asthmatic patients. These data demonstrated that a certain percentage of normodense eosinophils from asthmatics and normal subjects switched to hypodense after activation with platelet-activating factor or calcium ionophore. Furthermore; eosinophils from asthmatics switched to a greater degree than in normal subjects, suggesting that the normodense eosinophils in asthmatics become primed probably by endogenously released mediators.
AB - Eosinophils play a major role in the pathogenesis of bronchial asthma. In this study, we examined the density characteristics of blood eosinophils from 9 normal healthy individuals and 9 allergic asthmatic patients. Furthermore, the effect of platelet-activating factor, a potent mediator of inflammation, and calcium ionophore, A23187, on the density of normodense eosinophils (density > 1.085 g/ml) has also been examined. Initially, asthmatic patients had 27.0 ± 1.1% eosinophils of lighter density (density ≤ 1.081 g/ml), significantly greater than that in the normal individuals (7.5 ± 0.5%). After exposure to platelet-activating factor (1 μM) or calcium ionophore (A23187, 1 μg/ml), the normodense eosinophils switched to hypodense in both groups: 16.7 ± 2.1% and 54.2 ± 3.7%, respectively, in normal individuals, and 30.6 ± 5.7% and 77.4 ± 2.3%, respectively, in asthmatic patients. These data demonstrated that a certain percentage of normodense eosinophils from asthmatics and normal subjects switched to hypodense after activation with platelet-activating factor or calcium ionophore. Furthermore; eosinophils from asthmatics switched to a greater degree than in normal subjects, suggesting that the normodense eosinophils in asthmatics become primed probably by endogenously released mediators.
UR - http://www.scopus.com/inward/record.url?scp=0029775016&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029775016&partnerID=8YFLogxK
M3 - Article
VL - 33
SP - 213
EP - 219
JO - Journal of Asthma
JF - Journal of Asthma
SN - 0277-0903
IS - 4
ER -