Induction of synthesis of an antimicrobial peptide in the skin of the freeze-tolerant frog, Rana sylvatica, in response to environmental stimuli

Beverly Matutte, Kenneth B. Storey, Floyd C. Knoop, J. Michael Conlon

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

An extract of skin taken from specimens of the freeze-tolerant wood frog, Rana sylvatica, that were collected from cold (<7°C) ponds and maintained at 5°C lacked detectable antimicrobial activity. In contrast, an extract of skin taken from specimens maintained at 30°C for 3 weeks under laboratory conditions contained a high concentration (approximately 4 nmol/g) of a single antimicrobial peptide of the brevinin-1 family (FLPVVAGLAAKVLPSIICAVTKKC). The peptide inhibited growth of Escherichia coli (minimum inhibitory concentration 45 μM) and Staphylococcus aureus (minimum inhibitory concentration 7 μM). The data suggest that synthesis of the peptide is induced when the animal is in an environment that promotes the growth of microorganisms consistent with a role in the animal's defense strategy. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)135-138
Number of pages4
JournalFEBS Letters
Volume483
Issue number2-3
DOIs
StatePublished - Oct 20 2000

Fingerprint

Ranidae
Anura
Skin
Microbial Sensitivity Tests
Peptides
Animals
Ponds
Growth
Microorganisms
Escherichia coli
Staphylococcus aureus
Wood

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Induction of synthesis of an antimicrobial peptide in the skin of the freeze-tolerant frog, Rana sylvatica, in response to environmental stimuli. / Matutte, Beverly; Storey, Kenneth B.; Knoop, Floyd C.; Conlon, J. Michael.

In: FEBS Letters, Vol. 483, No. 2-3, 20.10.2000, p. 135-138.

Research output: Contribution to journalArticle

@article{7728af4a379f4c03b2c76e57b729db6f,
title = "Induction of synthesis of an antimicrobial peptide in the skin of the freeze-tolerant frog, Rana sylvatica, in response to environmental stimuli",
abstract = "An extract of skin taken from specimens of the freeze-tolerant wood frog, Rana sylvatica, that were collected from cold (<7°C) ponds and maintained at 5°C lacked detectable antimicrobial activity. In contrast, an extract of skin taken from specimens maintained at 30°C for 3 weeks under laboratory conditions contained a high concentration (approximately 4 nmol/g) of a single antimicrobial peptide of the brevinin-1 family (FLPVVAGLAAKVLPSIICAVTKKC). The peptide inhibited growth of Escherichia coli (minimum inhibitory concentration 45 μM) and Staphylococcus aureus (minimum inhibitory concentration 7 μM). The data suggest that synthesis of the peptide is induced when the animal is in an environment that promotes the growth of microorganisms consistent with a role in the animal's defense strategy. (C) 2000 Federation of European Biochemical Societies.",
author = "Beverly Matutte and Storey, {Kenneth B.} and Knoop, {Floyd C.} and Conlon, {J. Michael}",
year = "2000",
month = "10",
day = "20",
doi = "10.1016/S0014-5793(00)02102-5",
language = "English (US)",
volume = "483",
pages = "135--138",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Induction of synthesis of an antimicrobial peptide in the skin of the freeze-tolerant frog, Rana sylvatica, in response to environmental stimuli

AU - Matutte, Beverly

AU - Storey, Kenneth B.

AU - Knoop, Floyd C.

AU - Conlon, J. Michael

PY - 2000/10/20

Y1 - 2000/10/20

N2 - An extract of skin taken from specimens of the freeze-tolerant wood frog, Rana sylvatica, that were collected from cold (<7°C) ponds and maintained at 5°C lacked detectable antimicrobial activity. In contrast, an extract of skin taken from specimens maintained at 30°C for 3 weeks under laboratory conditions contained a high concentration (approximately 4 nmol/g) of a single antimicrobial peptide of the brevinin-1 family (FLPVVAGLAAKVLPSIICAVTKKC). The peptide inhibited growth of Escherichia coli (minimum inhibitory concentration 45 μM) and Staphylococcus aureus (minimum inhibitory concentration 7 μM). The data suggest that synthesis of the peptide is induced when the animal is in an environment that promotes the growth of microorganisms consistent with a role in the animal's defense strategy. (C) 2000 Federation of European Biochemical Societies.

AB - An extract of skin taken from specimens of the freeze-tolerant wood frog, Rana sylvatica, that were collected from cold (<7°C) ponds and maintained at 5°C lacked detectable antimicrobial activity. In contrast, an extract of skin taken from specimens maintained at 30°C for 3 weeks under laboratory conditions contained a high concentration (approximately 4 nmol/g) of a single antimicrobial peptide of the brevinin-1 family (FLPVVAGLAAKVLPSIICAVTKKC). The peptide inhibited growth of Escherichia coli (minimum inhibitory concentration 45 μM) and Staphylococcus aureus (minimum inhibitory concentration 7 μM). The data suggest that synthesis of the peptide is induced when the animal is in an environment that promotes the growth of microorganisms consistent with a role in the animal's defense strategy. (C) 2000 Federation of European Biochemical Societies.

UR - http://www.scopus.com/inward/record.url?scp=0034693283&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034693283&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(00)02102-5

DO - 10.1016/S0014-5793(00)02102-5

M3 - Article

VL - 483

SP - 135

EP - 138

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2-3

ER -