Induction of synthesis of an antimicrobial peptide in the skin of the freeze-tolerant frog, Rana sylvatica, in response to environmental stimuli

Beverly Matutte, Kenneth B. Storey, Floyd C. Knoop, J. Michael Conlon

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An extract of skin taken from specimens of the freeze-tolerant wood frog, Rana sylvatica, that were collected from cold (<7°C) ponds and maintained at 5°C lacked detectable antimicrobial activity. In contrast, an extract of skin taken from specimens maintained at 30°C for 3 weeks under laboratory conditions contained a high concentration (approximately 4 nmol/g) of a single antimicrobial peptide of the brevinin-1 family (FLPVVAGLAAKVLPSIICAVTKKC). The peptide inhibited growth of Escherichia coli (minimum inhibitory concentration 45 μM) and Staphylococcus aureus (minimum inhibitory concentration 7 μM). The data suggest that synthesis of the peptide is induced when the animal is in an environment that promotes the growth of microorganisms consistent with a role in the animal's defense strategy. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)135-138
Number of pages4
JournalFEBS Letters
Issue number2-3
StatePublished - Oct 20 2000


All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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