Inhibition of granulocyte function by steroids is not limited to corticoids - Studies with sex steroids

Dale E. Hammerschmidt, Ann C. Knabe, Peter T. Silberstein, Herbert R. Lamche, Patricia A. Coppo

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Noting that corticosteroid doses required for protection in shock models exceeded those required to saturate glucocorticoid receptors in mammalian cells, we postulated a nonspecific physicochemical effect of steroids upon the cell membrane, and therefore tested three noncorticoid steroids for their effects on granulocyte function. All three (conjugated equine estrogen, a synthetic progestogen, and a synthetic androgen) behaved in manner analogous to corticoids at similar concentrations, inhibiting granulocyte aggregation, chemotaxis, and chemiluminescence, as well as binding to the granulocytes of the synthetic oligopepitide agonist f-Met-Leu-Phe. Estrogen was further shown to reduce granulocyte membrane fluidity, assessed by electron paramagnetic resonance. We propose that the unique effects of extremely high-dose corticosteroids are not mediated via the glucocorticoid receptor, but result rather from physicochemical effects of the drugs upon the membranes of effector cells.

Original languageEnglish
Pages (from-to)277-284
Number of pages8
JournalInflammation
Volume12
Issue number3
DOIs
StatePublished - Jun 1988

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Granulocytes
Adrenal Cortex Hormones
Steroids
Glucocorticoid Receptors
Testosterone Congeners
Cell Membrane
Progesterone Congeners
Conjugated (USP) Estrogens
Membrane Fluidity
Electron Spin Resonance Spectroscopy
Chemotaxis
Luminescence
Shock
Estrogens
Inhibition (Psychology)
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Immunology
  • Cell Biology

Cite this

Inhibition of granulocyte function by steroids is not limited to corticoids - Studies with sex steroids. / Hammerschmidt, Dale E.; Knabe, Ann C.; Silberstein, Peter T.; Lamche, Herbert R.; Coppo, Patricia A.

In: Inflammation, Vol. 12, No. 3, 06.1988, p. 277-284.

Research output: Contribution to journalArticle

Hammerschmidt, Dale E. ; Knabe, Ann C. ; Silberstein, Peter T. ; Lamche, Herbert R. ; Coppo, Patricia A. / Inhibition of granulocyte function by steroids is not limited to corticoids - Studies with sex steroids. In: Inflammation. 1988 ; Vol. 12, No. 3. pp. 277-284.
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