Inhibition of potassium- and ischemia-evoked [3H] D-aspartate release from isolated bovine retina by cannabinoids

Catherine A. Opere, Wei Zheng, Min Zhao, Jin Lee, Kaustubh Kulkarni, Sunny Ohia

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We investigated the effect of cannabinoids on potassium chloride (K + )- and ischemia-induced [ 3 H]D-aspartate release from isolated bovine retinae. The superfusion method was employed for studies of [ 3 H]-neurotransmitter release. Cannabinoid receptor CB1 agonists, but not the CB2 agonist JWH 015, inhibited K + -induced [ 3 H]D-aspartate release from bovine retinae with the following rank order of activity: anandamide > ACEA > methanandamide > WIN 55,212-2. In the ischemic model, the rank order of activity was as follows: methanandamide > ACEA > WIN 55,212-2. The CB1 receptor antagonist AM 251 blocked inhibitory responses produced by cannabinoids in both experimental conditions. In conclusion, cannabinoids inhibit evoked [ 3 H]D-aspartate release from isolated bovine retinae via an effect on CB1 receptors.

Original languageEnglish
Pages (from-to)645-653
Number of pages9
JournalCurrent Eye Research
Volume31
Issue number7-8
DOIs
StatePublished - Aug 1 2006

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D-Aspartic Acid
Cannabinoid Receptor CB1
Cannabinoids
Retina
Potassium
Ischemia
Cannabinoid Receptor Agonists
Potassium Chloride
Neurotransmitter Agents
methanandamide
Win 55212-2

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems

Cite this

Inhibition of potassium- and ischemia-evoked [3H] D-aspartate release from isolated bovine retina by cannabinoids. / Opere, Catherine A.; Zheng, Wei; Zhao, Min; Lee, Jin; Kulkarni, Kaustubh; Ohia, Sunny.

In: Current Eye Research, Vol. 31, No. 7-8, 01.08.2006, p. 645-653.

Research output: Contribution to journalArticle

Opere, Catherine A. ; Zheng, Wei ; Zhao, Min ; Lee, Jin ; Kulkarni, Kaustubh ; Ohia, Sunny. / Inhibition of potassium- and ischemia-evoked [3H] D-aspartate release from isolated bovine retina by cannabinoids. In: Current Eye Research. 2006 ; Vol. 31, No. 7-8. pp. 645-653.
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