Inhibition of potassium- and ischemia-evoked [3H] D-aspartate release from isolated bovine retina by cannabinoids

Catherine Opere, Wei Zheng, Min Zhao, Jin Lee, Kaustubh Kulkarni, Sunny Ohia

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20 Scopus citations


We investigated the effect of cannabinoids on potassium chloride (K + )- and ischemia-induced [ 3 H]D-aspartate release from isolated bovine retinae. The superfusion method was employed for studies of [ 3 H]-neurotransmitter release. Cannabinoid receptor CB1 agonists, but not the CB2 agonist JWH 015, inhibited K + -induced [ 3 H]D-aspartate release from bovine retinae with the following rank order of activity: anandamide > ACEA > methanandamide > WIN 55,212-2. In the ischemic model, the rank order of activity was as follows: methanandamide > ACEA > WIN 55,212-2. The CB1 receptor antagonist AM 251 blocked inhibitory responses produced by cannabinoids in both experimental conditions. In conclusion, cannabinoids inhibit evoked [ 3 H]D-aspartate release from isolated bovine retinae via an effect on CB1 receptors.

Original languageEnglish (US)
Pages (from-to)645-653
Number of pages9
JournalCurrent Eye Research
Issue number7-8
StatePublished - Aug 1 2006


All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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