Inhibition of potassium- and ischemia-evoked [3H] D-aspartate release from isolated bovine retina by cannabinoids

Catherine Opere; Wei Zheng; Min Zhao; Jin Lee; Kaustubh Kulkarni; Sunny Ohia

doi:10.1080/02713680600762747

Inhibition of potassium- and ischemia-evoked [³H] D-aspartate release from isolated bovine retina by cannabinoids

Catherine Opere, Wei Zheng, Min Zhao, Jin Lee, Kaustubh Kulkarni, Sunny Ohia

Department of Pharmacy Sciences

Research output: Contribution to journal › Article

20 Scopus citations

Abstract

We investigated the effect of cannabinoids on potassium chloride (K + )- and ischemia-induced [ 3 H]D-aspartate release from isolated bovine retinae. The superfusion method was employed for studies of [ 3 H]-neurotransmitter release. Cannabinoid receptor CB1 agonists, but not the CB2 agonist JWH 015, inhibited K + -induced [ 3 H]D-aspartate release from bovine retinae with the following rank order of activity: anandamide > ACEA > methanandamide > WIN 55,212-2. In the ischemic model, the rank order of activity was as follows: methanandamide > ACEA > WIN 55,212-2. The CB1 receptor antagonist AM 251 blocked inhibitory responses produced by cannabinoids in both experimental conditions. In conclusion, cannabinoids inhibit evoked [ 3 H]D-aspartate release from isolated bovine retinae via an effect on CB1 receptors.

Original language	English (US)
Pages (from-to)	645-653
Number of pages	9
Journal	Current Eye Research
Volume	31
Issue number	7-8
DOIs	https://doi.org/10.1080/02713680600762747
State	Published - Aug 1 2006

All Science Journal Classification (ASJC) codes

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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Opere, C., Zheng, W., Zhao, M., Lee, J., Kulkarni, K., & Ohia, S. (2006). Inhibition of potassium- and ischemia-evoked [³H] D-aspartate release from isolated bovine retina by cannabinoids. Current Eye Research, 31(7-8), 645-653. https://doi.org/10.1080/02713680600762747

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title = "Inhibition of potassium- and ischemia-evoked [3H] D-aspartate release from isolated bovine retina by cannabinoids",

abstract = "We investigated the effect of cannabinoids on potassium chloride (K + )- and ischemia-induced [ 3 H]D-aspartate release from isolated bovine retinae. The superfusion method was employed for studies of [ 3 H]-neurotransmitter release. Cannabinoid receptor CB1 agonists, but not the CB2 agonist JWH 015, inhibited K + -induced [ 3 H]D-aspartate release from bovine retinae with the following rank order of activity: anandamide > ACEA > methanandamide > WIN 55,212-2. In the ischemic model, the rank order of activity was as follows: methanandamide > ACEA > WIN 55,212-2. The CB1 receptor antagonist AM 251 blocked inhibitory responses produced by cannabinoids in both experimental conditions. In conclusion, cannabinoids inhibit evoked [ 3 H]D-aspartate release from isolated bovine retinae via an effect on CB1 receptors.",

author = "Catherine Opere and Wei Zheng and Min Zhao and Jin Lee and Kaustubh Kulkarni and Sunny Ohia",

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N2 - We investigated the effect of cannabinoids on potassium chloride (K + )- and ischemia-induced [ 3 H]D-aspartate release from isolated bovine retinae. The superfusion method was employed for studies of [ 3 H]-neurotransmitter release. Cannabinoid receptor CB1 agonists, but not the CB2 agonist JWH 015, inhibited K + -induced [ 3 H]D-aspartate release from bovine retinae with the following rank order of activity: anandamide > ACEA > methanandamide > WIN 55,212-2. In the ischemic model, the rank order of activity was as follows: methanandamide > ACEA > WIN 55,212-2. The CB1 receptor antagonist AM 251 blocked inhibitory responses produced by cannabinoids in both experimental conditions. In conclusion, cannabinoids inhibit evoked [ 3 H]D-aspartate release from isolated bovine retinae via an effect on CB1 receptors.

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