TY - JOUR
T1 - Inhibition of potassium- and ischemia-evoked [3H] D-aspartate release from isolated bovine retina by cannabinoids
AU - Opere, Catherine
AU - Zheng, Wei
AU - Zhao, Min
AU - Lee, Jin
AU - Kulkarni, Kaustubh
AU - Ohia, Sunny
PY - 2006/8/1
Y1 - 2006/8/1
N2 - We investigated the effect of cannabinoids on potassium chloride (K + )- and ischemia-induced [ 3 H]D-aspartate release from isolated bovine retinae. The superfusion method was employed for studies of [ 3 H]-neurotransmitter release. Cannabinoid receptor CB1 agonists, but not the CB2 agonist JWH 015, inhibited K + -induced [ 3 H]D-aspartate release from bovine retinae with the following rank order of activity: anandamide > ACEA > methanandamide > WIN 55,212-2. In the ischemic model, the rank order of activity was as follows: methanandamide > ACEA > WIN 55,212-2. The CB1 receptor antagonist AM 251 blocked inhibitory responses produced by cannabinoids in both experimental conditions. In conclusion, cannabinoids inhibit evoked [ 3 H]D-aspartate release from isolated bovine retinae via an effect on CB1 receptors.
AB - We investigated the effect of cannabinoids on potassium chloride (K + )- and ischemia-induced [ 3 H]D-aspartate release from isolated bovine retinae. The superfusion method was employed for studies of [ 3 H]-neurotransmitter release. Cannabinoid receptor CB1 agonists, but not the CB2 agonist JWH 015, inhibited K + -induced [ 3 H]D-aspartate release from bovine retinae with the following rank order of activity: anandamide > ACEA > methanandamide > WIN 55,212-2. In the ischemic model, the rank order of activity was as follows: methanandamide > ACEA > WIN 55,212-2. The CB1 receptor antagonist AM 251 blocked inhibitory responses produced by cannabinoids in both experimental conditions. In conclusion, cannabinoids inhibit evoked [ 3 H]D-aspartate release from isolated bovine retinae via an effect on CB1 receptors.
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U2 - 10.1080/02713680600762747
DO - 10.1080/02713680600762747
M3 - Article
C2 - 16877273
AN - SCOPUS:33746796735
VL - 31
SP - 645
EP - 653
JO - Current Eye Research
JF - Current Eye Research
SN - 0271-3683
IS - 7-8
ER -