Inhibitory action of novel hydrogen sulfide donors on bovine isolated posterior ciliary arteries

Madhura Kulkarni-Chitnis, Ya Fatou Njie-Mbye, Leah Mitchell, Jenaye Robinson, Matthew Whiteman, Mark E. Wood, Catherine A. Opere, Sunny E. Ohia

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Abstract

In the present study, we investigate the inhibitory effect of novel H2S donors, AP67 and AP72 on isolated bovine posterior ciliary arteries (PCAs) under conditions of tone induced by an adrenoceptor agonist. Furthermore, we examined the possible mechanisms underlying the AP67- and AP72-induced relaxations. Isolated bovine PCA were set up for measurement of isometric tension in organ baths containing oxygenated Krebs solution. The relaxant action of H2S donors was studied on phenylephrine-induced tone in the absence or presence of enzyme inhibitors for the following pathways: cyclooxygenase (COX); H2S; nitric oxide and the ATP-sensitive K+ (KATP) channel. The H2S donors, NaSH (1nM - 10μM), AP67 (1nM - 10μM) and AP72 (10nM - 1μM) elicited a concentration-dependent relaxation of phenylephrine-induced tone in isolated bovine PCA. While the COX inhibitor, flurbiprofen (3μM) blocked significantly (p2S biosynthesis caused significant (pATP channel antagonist, glibenclamide (300μM) and the NO synthase inhibitor, l-NAME (100μM) significantly attenuated (p2S donors can relax pre-contracted isolated bovine PCA, an effect dependent on endogenous production of H2S. The inhibitory action of only AP67 on pre-contracted PCA may involve the production of inhibitory endogenous prostanoids. Furthermore, the observed inhibitory action of H2S donors on PCA may depend on the endogenous biosynthesis of NO and by an action of KATP channels.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalExperimental Eye Research
Volume134
DOIs
StatePublished - May 1 2015

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Ciliary Arteries
Hydrogen Sulfide
KATP Channels
Phenylephrine
Flurbiprofen
Cyclooxygenase Inhibitors
Glyburide
Enzyme Inhibitors
Prostaglandin-Endoperoxide Synthases
Baths
Nitric Oxide Synthase
Adrenergic Receptors
Prostaglandins
Nitric Oxide
Adenosine Triphosphate

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Kulkarni-Chitnis, M., Njie-Mbye, Y. F., Mitchell, L., Robinson, J., Whiteman, M., Wood, M. E., ... Ohia, S. E. (2015). Inhibitory action of novel hydrogen sulfide donors on bovine isolated posterior ciliary arteries. Experimental Eye Research, 134, 73-79. https://doi.org/10.1016/j.exer.2015.04.001

Inhibitory action of novel hydrogen sulfide donors on bovine isolated posterior ciliary arteries. / Kulkarni-Chitnis, Madhura; Njie-Mbye, Ya Fatou; Mitchell, Leah; Robinson, Jenaye; Whiteman, Matthew; Wood, Mark E.; Opere, Catherine A.; Ohia, Sunny E.

In: Experimental Eye Research, Vol. 134, 01.05.2015, p. 73-79.

Research output: Contribution to journalArticle

Kulkarni-Chitnis, M, Njie-Mbye, YF, Mitchell, L, Robinson, J, Whiteman, M, Wood, ME, Opere, CA & Ohia, SE 2015, 'Inhibitory action of novel hydrogen sulfide donors on bovine isolated posterior ciliary arteries', Experimental Eye Research, vol. 134, pp. 73-79. https://doi.org/10.1016/j.exer.2015.04.001
Kulkarni-Chitnis M, Njie-Mbye YF, Mitchell L, Robinson J, Whiteman M, Wood ME et al. Inhibitory action of novel hydrogen sulfide donors on bovine isolated posterior ciliary arteries. Experimental Eye Research. 2015 May 1;134:73-79. https://doi.org/10.1016/j.exer.2015.04.001
Kulkarni-Chitnis, Madhura ; Njie-Mbye, Ya Fatou ; Mitchell, Leah ; Robinson, Jenaye ; Whiteman, Matthew ; Wood, Mark E. ; Opere, Catherine A. ; Ohia, Sunny E. / Inhibitory action of novel hydrogen sulfide donors on bovine isolated posterior ciliary arteries. In: Experimental Eye Research. 2015 ; Vol. 134. pp. 73-79.
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