TY - JOUR
T1 - Inhibitory action of novel hydrogen sulfide donors on bovine isolated posterior ciliary arteries
AU - Kulkarni-Chitnis, Madhura
AU - Njie-Mbye, Ya Fatou
AU - Mitchell, Leah
AU - Robinson, Jenaye
AU - Whiteman, Matthew
AU - Wood, Mark E.
AU - Opere, Catherine A.
AU - Ohia, Sunny E.
N1 - Funding Information:
This work was supported in part by National Institutes of Health/National Eye Institute Grant R15EY022215-01 .
PY - 2015/5/1
Y1 - 2015/5/1
N2 - In the present study, we investigate the inhibitory effect of novel H2S donors, AP67 and AP72 on isolated bovine posterior ciliary arteries (PCAs) under conditions of tone induced by an adrenoceptor agonist. Furthermore, we examined the possible mechanisms underlying the AP67- and AP72-induced relaxations. Isolated bovine PCA were set up for measurement of isometric tension in organ baths containing oxygenated Krebs solution. The relaxant action of H2S donors was studied on phenylephrine-induced tone in the absence or presence of enzyme inhibitors for the following pathways: cyclooxygenase (COX); H2S; nitric oxide and the ATP-sensitive K+ (KATP) channel. The H2S donors, NaSH (1nM - 10μM), AP67 (1nM - 10μM) and AP72 (10nM - 1μM) elicited a concentration-dependent relaxation of phenylephrine-induced tone in isolated bovine PCA. While the COX inhibitor, flurbiprofen (3μM) blocked significantly (p2S biosynthesis caused significant (pATP channel antagonist, glibenclamide (300μM) and the NO synthase inhibitor, l-NAME (100μM) significantly attenuated (p2S donors can relax pre-contracted isolated bovine PCA, an effect dependent on endogenous production of H2S. The inhibitory action of only AP67 on pre-contracted PCA may involve the production of inhibitory endogenous prostanoids. Furthermore, the observed inhibitory action of H2S donors on PCA may depend on the endogenous biosynthesis of NO and by an action of KATP channels.
AB - In the present study, we investigate the inhibitory effect of novel H2S donors, AP67 and AP72 on isolated bovine posterior ciliary arteries (PCAs) under conditions of tone induced by an adrenoceptor agonist. Furthermore, we examined the possible mechanisms underlying the AP67- and AP72-induced relaxations. Isolated bovine PCA were set up for measurement of isometric tension in organ baths containing oxygenated Krebs solution. The relaxant action of H2S donors was studied on phenylephrine-induced tone in the absence or presence of enzyme inhibitors for the following pathways: cyclooxygenase (COX); H2S; nitric oxide and the ATP-sensitive K+ (KATP) channel. The H2S donors, NaSH (1nM - 10μM), AP67 (1nM - 10μM) and AP72 (10nM - 1μM) elicited a concentration-dependent relaxation of phenylephrine-induced tone in isolated bovine PCA. While the COX inhibitor, flurbiprofen (3μM) blocked significantly (p2S biosynthesis caused significant (pATP channel antagonist, glibenclamide (300μM) and the NO synthase inhibitor, l-NAME (100μM) significantly attenuated (p2S donors can relax pre-contracted isolated bovine PCA, an effect dependent on endogenous production of H2S. The inhibitory action of only AP67 on pre-contracted PCA may involve the production of inhibitory endogenous prostanoids. Furthermore, the observed inhibitory action of H2S donors on PCA may depend on the endogenous biosynthesis of NO and by an action of KATP channels.
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U2 - 10.1016/j.exer.2015.04.001
DO - 10.1016/j.exer.2015.04.001
M3 - Article
C2 - 25845295
AN - SCOPUS:84927556471
VL - 134
SP - 73
EP - 79
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 0014-4835
ER -