Abstract
The effect of terfenadine on histamine release from human basophils and LTC4 production and release from human eosinophils was evaluated. Eosinophils and basophils were obtained by discontinuous gradient centrifugation of the peripheral blood of atopic asthma patients who were off medication. Anti-IgE-induced histamine release from human basophils was significantly inhibited by terfenadine. Maximum inhibition was obtained at 1 x 10-5 M terfenadine (percentage inhibition = 57.0 ± 20.1; P <0.05). However, only the highest dose of terfenadine used in this study, i.e. 2 x 10-5 M, significantly inhibited calcium ionophore (A23187)-induced histamine release from human basophils (percentage inhibition = 40.0 ± 14.6; P <0.05), and LTC4 production from human eosinophils (percentage inhibition 59.8 ± 9.9; P <0.05). These findings demonstrate that terfenadine, in addition to its known antihistaminic property, also has an inhibitory effect on chemical mediator release.
| Original language | English |
|---|---|
| Pages (from-to) | 515-520 |
| Number of pages | 6 |
| Journal | Clinical and Experimental Allergy |
| Volume | 19 |
| Issue number | 5 |
| State | Published - 1989 |
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All Science Journal Classification (ASJC) codes
- Immunology
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Inhibitory effect of terfenadine on mediator release from human blood basophils and eosinophils. / Nabe, M.; Agrawal, Devendra K.; Sarmiento, E. U.; Townley, R. G.
In: Clinical and Experimental Allergy, Vol. 19, No. 5, 1989, p. 515-520.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibitory effect of terfenadine on mediator release from human blood basophils and eosinophils
AU - Nabe, M.
AU - Agrawal, Devendra K.
AU - Sarmiento, E. U.
AU - Townley, R. G.
PY - 1989
Y1 - 1989
N2 - The effect of terfenadine on histamine release from human basophils and LTC4 production and release from human eosinophils was evaluated. Eosinophils and basophils were obtained by discontinuous gradient centrifugation of the peripheral blood of atopic asthma patients who were off medication. Anti-IgE-induced histamine release from human basophils was significantly inhibited by terfenadine. Maximum inhibition was obtained at 1 x 10-5 M terfenadine (percentage inhibition = 57.0 ± 20.1; P <0.05). However, only the highest dose of terfenadine used in this study, i.e. 2 x 10-5 M, significantly inhibited calcium ionophore (A23187)-induced histamine release from human basophils (percentage inhibition = 40.0 ± 14.6; P <0.05), and LTC4 production from human eosinophils (percentage inhibition 59.8 ± 9.9; P <0.05). These findings demonstrate that terfenadine, in addition to its known antihistaminic property, also has an inhibitory effect on chemical mediator release.
AB - The effect of terfenadine on histamine release from human basophils and LTC4 production and release from human eosinophils was evaluated. Eosinophils and basophils were obtained by discontinuous gradient centrifugation of the peripheral blood of atopic asthma patients who were off medication. Anti-IgE-induced histamine release from human basophils was significantly inhibited by terfenadine. Maximum inhibition was obtained at 1 x 10-5 M terfenadine (percentage inhibition = 57.0 ± 20.1; P <0.05). However, only the highest dose of terfenadine used in this study, i.e. 2 x 10-5 M, significantly inhibited calcium ionophore (A23187)-induced histamine release from human basophils (percentage inhibition = 40.0 ± 14.6; P <0.05), and LTC4 production from human eosinophils (percentage inhibition 59.8 ± 9.9; P <0.05). These findings demonstrate that terfenadine, in addition to its known antihistaminic property, also has an inhibitory effect on chemical mediator release.
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M3 - Article
VL - 19
SP - 515
EP - 520
JO - Clinical and Experimental Allergy
JF - Clinical and Experimental Allergy
SN - 0954-7894
IS - 5
ER -