Injection of the sciatic nerve with TMEV

A new model for peripheral nerve demyelination

Kristen M. Drescher, Steven M. Tracy

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Demyelination of the human peripheral nervous system (PNS) can be caused by diverse mechanisms including viral infection. Despite association of several viruses with the development of peripheral demyelination, animal models of the condition have been limited to disease that is either autoimmune or genetic in origin. We describe here a model of PNS demyelination based on direct injection of sciatic nerves of mice with the cardiovirus, Theiler's murine encephalomyelitis virus (TMEV). Sciatic nerves of FVB mice develop inflammatory cell infiltration following TMEV injection. Schwann cells and macrophages are infected with TMEV. Viral replication is observed initially in the sciatic nerves and subsequently the spinal cord. Sciatic nerves are demyelinated by day 5 post-inoculation (p.i.). Injecting sciatic nerves of scid mice resulted in increased levels of virus recovered from the sciatic nerve and spinal cord relative to FVB mice. Demyelination also occurred in scid mice and by 12 days p.i., hindlimbs were paralyzed. This new model of virus-induced peripheral demyelination may be used to dissect processes involved in protection of the PNS from viral insult and to study the early phases of lesion development.

Original languageEnglish
Pages (from-to)233-242
Number of pages10
JournalVirology
Volume359
Issue number1
DOIs
StatePublished - Mar 1 2007

Fingerprint

Theilovirus
Demyelinating Diseases
Sciatic Nerve
Peripheral Nerves
Injections
Peripheral Nervous System
Viruses
Cardiovirus
Spinal Cord
Schwann Cells
Virus Diseases
Hindlimb
Animal Models
Macrophages

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Injection of the sciatic nerve with TMEV : A new model for peripheral nerve demyelination. / Drescher, Kristen M.; Tracy, Steven M.

In: Virology, Vol. 359, No. 1, 01.03.2007, p. 233-242.

Research output: Contribution to journalArticle

@article{af689235eb07408693650099f08c7d69,
title = "Injection of the sciatic nerve with TMEV: A new model for peripheral nerve demyelination",
abstract = "Demyelination of the human peripheral nervous system (PNS) can be caused by diverse mechanisms including viral infection. Despite association of several viruses with the development of peripheral demyelination, animal models of the condition have been limited to disease that is either autoimmune or genetic in origin. We describe here a model of PNS demyelination based on direct injection of sciatic nerves of mice with the cardiovirus, Theiler's murine encephalomyelitis virus (TMEV). Sciatic nerves of FVB mice develop inflammatory cell infiltration following TMEV injection. Schwann cells and macrophages are infected with TMEV. Viral replication is observed initially in the sciatic nerves and subsequently the spinal cord. Sciatic nerves are demyelinated by day 5 post-inoculation (p.i.). Injecting sciatic nerves of scid mice resulted in increased levels of virus recovered from the sciatic nerve and spinal cord relative to FVB mice. Demyelination also occurred in scid mice and by 12 days p.i., hindlimbs were paralyzed. This new model of virus-induced peripheral demyelination may be used to dissect processes involved in protection of the PNS from viral insult and to study the early phases of lesion development.",
author = "Drescher, {Kristen M.} and Tracy, {Steven M.}",
year = "2007",
month = "3",
day = "1",
doi = "10.1016/j.virol.2006.09.009",
language = "English",
volume = "359",
pages = "233--242",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Injection of the sciatic nerve with TMEV

T2 - A new model for peripheral nerve demyelination

AU - Drescher, Kristen M.

AU - Tracy, Steven M.

PY - 2007/3/1

Y1 - 2007/3/1

N2 - Demyelination of the human peripheral nervous system (PNS) can be caused by diverse mechanisms including viral infection. Despite association of several viruses with the development of peripheral demyelination, animal models of the condition have been limited to disease that is either autoimmune or genetic in origin. We describe here a model of PNS demyelination based on direct injection of sciatic nerves of mice with the cardiovirus, Theiler's murine encephalomyelitis virus (TMEV). Sciatic nerves of FVB mice develop inflammatory cell infiltration following TMEV injection. Schwann cells and macrophages are infected with TMEV. Viral replication is observed initially in the sciatic nerves and subsequently the spinal cord. Sciatic nerves are demyelinated by day 5 post-inoculation (p.i.). Injecting sciatic nerves of scid mice resulted in increased levels of virus recovered from the sciatic nerve and spinal cord relative to FVB mice. Demyelination also occurred in scid mice and by 12 days p.i., hindlimbs were paralyzed. This new model of virus-induced peripheral demyelination may be used to dissect processes involved in protection of the PNS from viral insult and to study the early phases of lesion development.

AB - Demyelination of the human peripheral nervous system (PNS) can be caused by diverse mechanisms including viral infection. Despite association of several viruses with the development of peripheral demyelination, animal models of the condition have been limited to disease that is either autoimmune or genetic in origin. We describe here a model of PNS demyelination based on direct injection of sciatic nerves of mice with the cardiovirus, Theiler's murine encephalomyelitis virus (TMEV). Sciatic nerves of FVB mice develop inflammatory cell infiltration following TMEV injection. Schwann cells and macrophages are infected with TMEV. Viral replication is observed initially in the sciatic nerves and subsequently the spinal cord. Sciatic nerves are demyelinated by day 5 post-inoculation (p.i.). Injecting sciatic nerves of scid mice resulted in increased levels of virus recovered from the sciatic nerve and spinal cord relative to FVB mice. Demyelination also occurred in scid mice and by 12 days p.i., hindlimbs were paralyzed. This new model of virus-induced peripheral demyelination may be used to dissect processes involved in protection of the PNS from viral insult and to study the early phases of lesion development.

UR - http://www.scopus.com/inward/record.url?scp=33846857968&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846857968&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2006.09.009

DO - 10.1016/j.virol.2006.09.009

M3 - Article

VL - 359

SP - 233

EP - 242

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -