Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina

Madhura Kulkarni-Chitnis, Leah Mitchell-Bush, Remmington Belford, Jenaye Robinson, Catherine A. Opere, Sunny E. Ohia, Ya Fatou N. Mbye

Research output: Contribution to journalArticle

Abstract

Purpose: Nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S) are physiologically relevant gaseous neurotransmitters that are endogenously produced in mammalian tissues. In the present study, we investigated the possibility that NO and CO can regulate the endogenous levels of H2S in bovine isolated neural retina. Methods: Isolated bovine neural retina were homogenized and tissue homogenates were treated with a NO synthase inhibitor, NO donor, heme oxygenase-1 inhibitor, and/donor. H2S concentrations in bovine retinal homogenates were measured using a well-established colorimetric assay. Results: L-NAME (300 nM-500 μM) caused a concentration-dependent decrease in basal endogenous levels of H2S by 86.2%. On the other hand, SNP (10-300 μM) elicited a concentration-related increase in H2S levels from 18.3 nM/mg of protein to 65.7 nM/mg of protein. ZnPP-IX (300 nM-10 μM) caused a concentration-dependent increase in the endogenous production of H2S whereas hemin (300 nM-20 μM) attenuated the basal levels of H2S. Conclusion: We conclude that changes in the biosynthesis and availability of both NO and CO can interfere with the pathway/s involved in the production of H2S in the retina. The demonstrated ability of NO, CO and H2S to interact in the mammalian retina affirms a physiological/pharmacological role for these gaseous mediators in the eye.

Original languageEnglish (US)
Pages (from-to)104-115
Number of pages12
JournalAIMS Neuroscience
Volume6
Issue number3
DOIs
StatePublished - Jan 1 2019

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Hydrogen Sulfide
Carbon Monoxide
Retina
Nitric Oxide
Gasotransmitters
Hemin
Heme Oxygenase-1
Nitric Oxide Donors
NG-Nitroarginine Methyl Ester
Nitric Oxide Synthase
Single Nucleotide Polymorphism
Neurotransmitter Agents
Proteins
Pharmacology

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina. / Kulkarni-Chitnis, Madhura; Mitchell-Bush, Leah; Belford, Remmington; Robinson, Jenaye; Opere, Catherine A.; Ohia, Sunny E.; Mbye, Ya Fatou N.

In: AIMS Neuroscience, Vol. 6, No. 3, 01.01.2019, p. 104-115.

Research output: Contribution to journalArticle

Kulkarni-Chitnis, M, Mitchell-Bush, L, Belford, R, Robinson, J, Opere, CA, Ohia, SE & Mbye, YFN 2019, 'Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina', AIMS Neuroscience, vol. 6, no. 3, pp. 104-115. https://doi.org/10.3934/NEUROSCIENCE.2019.3.104
Kulkarni-Chitnis, Madhura ; Mitchell-Bush, Leah ; Belford, Remmington ; Robinson, Jenaye ; Opere, Catherine A. ; Ohia, Sunny E. ; Mbye, Ya Fatou N. / Interaction between hydrogen sulfide, nitric oxide, and carbon monoxide pathways in the bovine isolated retina. In: AIMS Neuroscience. 2019 ; Vol. 6, No. 3. pp. 104-115.
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AU - Opere, Catherine A.

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