Intracellular anions as the voltage sensor of prestin, the outer hair cell motor protein

D. Oliver, David Z. He, N. Klöcker, J. Ludwig, U. Schulte, S. Waldegger, J. P. Ruppersberg, P. Dallos, B. Fakler

Research output: Contribution to journalArticle

302 Citations (Scopus)

Abstract

Outer hair cells (OHCs) of the mammalian cochlea actively change their cell length in response to changes in membrane potential. This electromotility, thought to be the basis of cochlear amplification, is mediated by a voltage-sensitive motor molecule recently identified as the membrane protein prestin. Here, we show that voltage sensitivity is conferred to prestin by the intracellular anions chloride and bicarbonate. Removal of these anions abolished fast voltage-dependent motility, as well as the characteristic nonlinear charge movement ("gating currents") driving the underlying structural rearrangements of the protein. The results support a model in which anions act as extrinsic voltage sensors, which bind to the prestin molecule and thus trigger the conformational changes required for motility of OHCs.

Original languageEnglish
Pages (from-to)2340-2343
Number of pages4
JournalScience
Volume292
Issue number5525
DOIs
StatePublished - Jun 22 2001
Externally publishedYes

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Outer Auditory Hair Cells
Anions
Cochlea
Proteins
Bicarbonates
Membrane Potentials
Chlorides
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

Oliver, D., He, D. Z., Klöcker, N., Ludwig, J., Schulte, U., Waldegger, S., ... Fakler, B. (2001). Intracellular anions as the voltage sensor of prestin, the outer hair cell motor protein. Science, 292(5525), 2340-2343. https://doi.org/10.1126/science.1060939

Intracellular anions as the voltage sensor of prestin, the outer hair cell motor protein. / Oliver, D.; He, David Z.; Klöcker, N.; Ludwig, J.; Schulte, U.; Waldegger, S.; Ruppersberg, J. P.; Dallos, P.; Fakler, B.

In: Science, Vol. 292, No. 5525, 22.06.2001, p. 2340-2343.

Research output: Contribution to journalArticle

Oliver, D, He, DZ, Klöcker, N, Ludwig, J, Schulte, U, Waldegger, S, Ruppersberg, JP, Dallos, P & Fakler, B 2001, 'Intracellular anions as the voltage sensor of prestin, the outer hair cell motor protein', Science, vol. 292, no. 5525, pp. 2340-2343. https://doi.org/10.1126/science.1060939
Oliver D, He DZ, Klöcker N, Ludwig J, Schulte U, Waldegger S et al. Intracellular anions as the voltage sensor of prestin, the outer hair cell motor protein. Science. 2001 Jun 22;292(5525):2340-2343. https://doi.org/10.1126/science.1060939
Oliver, D. ; He, David Z. ; Klöcker, N. ; Ludwig, J. ; Schulte, U. ; Waldegger, S. ; Ruppersberg, J. P. ; Dallos, P. ; Fakler, B. / Intracellular anions as the voltage sensor of prestin, the outer hair cell motor protein. In: Science. 2001 ; Vol. 292, No. 5525. pp. 2340-2343.
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