Invasion of human fallopian tube epithelium by Escherichia coli expressing combinations of a gonococcal porin, opacity-associated protein, and chimeric lipo-oligosaccharide

Gary L. Gorby, A. F. Ehrhardt, M. A. Apicella, C. Elkins

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The transepithelial migration of Escherichia coli that expressed all possible combinations of a plasmid-encoded gonococcal porin (Por), opacity-associated protein (Opa), and 3F11 lipo-oligosaccharide (LOS) epitope was investigated. Surface expression of Por mediated selective changes in E. coli antibiotic susceptibility, and coexpression of Opa and the 3F11 LOS epitope mediated bacterial clumping (P <.01). In the human fallopian tube organ-culture model, Opa-producing variants attached up to 44-fold better than control bacteria (P <.01), and Por-producing variants exceeded submucosal invasion of control bacteria by 500-fold (P <.01). Opa and Por each facilitated intracellular invasion 20-40-fold (P <.01). In dual expresser variants, the 3F11 LOS epitope markedly reduced attachment and invasion mediated by Opa or Por. The LOS inhibitory effect was curbed when all 3 factors were expressed, which suggests an additional interaction of the 3 factors at the bacterial surface. Por, Opa, and LOS play important roles in Neisseria gonorrhoeae trafficking across human fallopian tube epithelium.

Original languageEnglish
Pages (from-to)460-472
Number of pages13
JournalJournal of Infectious Diseases
Volume184
Issue number4
DOIs
StatePublished - Aug 15 2001

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Porins
Fallopian Tubes
Oligosaccharides
Epithelium
Escherichia coli
Epitopes
Human Trafficking
Bacteria
Transendothelial and Transepithelial Migration
Neisseria gonorrhoeae
Organ Culture Techniques
opacity proteins
Plasmids
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Invasion of human fallopian tube epithelium by Escherichia coli expressing combinations of a gonococcal porin, opacity-associated protein, and chimeric lipo-oligosaccharide. / Gorby, Gary L.; Ehrhardt, A. F.; Apicella, M. A.; Elkins, C.

In: Journal of Infectious Diseases, Vol. 184, No. 4, 15.08.2001, p. 460-472.

Research output: Contribution to journalArticle

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