Abstract
The transepithelial migration of Escherichia coli that expressed all possible combinations of a plasmid-encoded gonococcal porin (Por), opacity-associated protein (Opa), and 3F11 lipo-oligosaccharide (LOS) epitope was investigated. Surface expression of Por mediated selective changes in E. coli antibiotic susceptibility, and coexpression of Opa and the 3F11 LOS epitope mediated bacterial clumping (P <.01). In the human fallopian tube organ-culture model, Opa-producing variants attached up to 44-fold better than control bacteria (P <.01), and Por-producing variants exceeded submucosal invasion of control bacteria by 500-fold (P <.01). Opa and Por each facilitated intracellular invasion 20-40-fold (P <.01). In dual expresser variants, the 3F11 LOS epitope markedly reduced attachment and invasion mediated by Opa or Por. The LOS inhibitory effect was curbed when all 3 factors were expressed, which suggests an additional interaction of the 3 factors at the bacterial surface. Por, Opa, and LOS play important roles in Neisseria gonorrhoeae trafficking across human fallopian tube epithelium.
| Original language | English |
|---|---|
| Pages (from-to) | 460-472 |
| Number of pages | 13 |
| Journal | Journal of Infectious Diseases |
| Volume | 184 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 15 2001 |
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All Science Journal Classification (ASJC) codes
- Public Health, Environmental and Occupational Health
- Immunology
Cite this
Invasion of human fallopian tube epithelium by Escherichia coli expressing combinations of a gonococcal porin, opacity-associated protein, and chimeric lipo-oligosaccharide. / Gorby, Gary L.; Ehrhardt, A. F.; Apicella, M. A.; Elkins, C.
In: Journal of Infectious Diseases, Vol. 184, No. 4, 15.08.2001, p. 460-472.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Invasion of human fallopian tube epithelium by Escherichia coli expressing combinations of a gonococcal porin, opacity-associated protein, and chimeric lipo-oligosaccharide
AU - Gorby, Gary L.
AU - Ehrhardt, A. F.
AU - Apicella, M. A.
AU - Elkins, C.
PY - 2001/8/15
Y1 - 2001/8/15
N2 - The transepithelial migration of Escherichia coli that expressed all possible combinations of a plasmid-encoded gonococcal porin (Por), opacity-associated protein (Opa), and 3F11 lipo-oligosaccharide (LOS) epitope was investigated. Surface expression of Por mediated selective changes in E. coli antibiotic susceptibility, and coexpression of Opa and the 3F11 LOS epitope mediated bacterial clumping (P <.01). In the human fallopian tube organ-culture model, Opa-producing variants attached up to 44-fold better than control bacteria (P <.01), and Por-producing variants exceeded submucosal invasion of control bacteria by 500-fold (P <.01). Opa and Por each facilitated intracellular invasion 20-40-fold (P <.01). In dual expresser variants, the 3F11 LOS epitope markedly reduced attachment and invasion mediated by Opa or Por. The LOS inhibitory effect was curbed when all 3 factors were expressed, which suggests an additional interaction of the 3 factors at the bacterial surface. Por, Opa, and LOS play important roles in Neisseria gonorrhoeae trafficking across human fallopian tube epithelium.
AB - The transepithelial migration of Escherichia coli that expressed all possible combinations of a plasmid-encoded gonococcal porin (Por), opacity-associated protein (Opa), and 3F11 lipo-oligosaccharide (LOS) epitope was investigated. Surface expression of Por mediated selective changes in E. coli antibiotic susceptibility, and coexpression of Opa and the 3F11 LOS epitope mediated bacterial clumping (P <.01). In the human fallopian tube organ-culture model, Opa-producing variants attached up to 44-fold better than control bacteria (P <.01), and Por-producing variants exceeded submucosal invasion of control bacteria by 500-fold (P <.01). Opa and Por each facilitated intracellular invasion 20-40-fold (P <.01). In dual expresser variants, the 3F11 LOS epitope markedly reduced attachment and invasion mediated by Opa or Por. The LOS inhibitory effect was curbed when all 3 factors were expressed, which suggests an additional interaction of the 3 factors at the bacterial surface. Por, Opa, and LOS play important roles in Neisseria gonorrhoeae trafficking across human fallopian tube epithelium.
UR - http://www.scopus.com/inward/record.url?scp=0035882505&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035882505&partnerID=8YFLogxK
U2 - 10.1086/322784
DO - 10.1086/322784
M3 - Article
C2 - 11471104
AN - SCOPUS:0035882505
VL - 184
SP - 460
EP - 472
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 4
ER -