TY - JOUR
T1 - Invasion of human fallopian tube epithelium by Escherichia coli expressing combinations of a gonococcal porin, opacity-associated protein, and chimeric lipo-oligosaccharide
AU - Gorby, Gary L.
AU - Ehrhardt, Anton F.
AU - Apicella, Michael A.
AU - Elkins, Christopher
N1 - Funding Information:
Received 9 March 2001; revised 1 May 2001; electronically published 25 July 2001. Presented in part: 12th International Pathogenic Neisseria Conference, Galveston, Texas, 12–17 November 2000 (abstract 168). Guidelines of the US Department of Health and Human Services and of the authors’ institutions were followed in obtaining and using human fallopian tube tissue from pathologic surgical specimens. Financial support: National Institute of Allergy and Infectious Diseases (award AI36893-05) and the Veterans Administration Merit Review Program. The authors have no commercial or other association that might pose a conflict of interest with the reported data. a Present affiliation: Bristol-Myers Squibb, Princeton, New Jersey. Reprints or correspondence: Dr. Gary L. Gorby, Section of Infectious Diseases, Omaha VA Medical Center, 4101 Woolworth Ave., Omaha, NE 68105 (gorby@omaha.va.gov).
PY - 2001/8/15
Y1 - 2001/8/15
N2 - The transepithelial migration of Escherichia coli that expressed all possible combinations of a plasmid-encoded gonococcal porin (Por), opacity-associated protein (Opa), and 3F11 lipo-oligosaccharide (LOS) epitope was investigated. Surface expression of Por mediated selective changes in E. coli antibiotic susceptibility, and coexpression of Opa and the 3F11 LOS epitope mediated bacterial clumping (P <.01). In the human fallopian tube organ-culture model, Opa-producing variants attached up to 44-fold better than control bacteria (P <.01), and Por-producing variants exceeded submucosal invasion of control bacteria by 500-fold (P <.01). Opa and Por each facilitated intracellular invasion 20-40-fold (P <.01). In dual expresser variants, the 3F11 LOS epitope markedly reduced attachment and invasion mediated by Opa or Por. The LOS inhibitory effect was curbed when all 3 factors were expressed, which suggests an additional interaction of the 3 factors at the bacterial surface. Por, Opa, and LOS play important roles in Neisseria gonorrhoeae trafficking across human fallopian tube epithelium.
AB - The transepithelial migration of Escherichia coli that expressed all possible combinations of a plasmid-encoded gonococcal porin (Por), opacity-associated protein (Opa), and 3F11 lipo-oligosaccharide (LOS) epitope was investigated. Surface expression of Por mediated selective changes in E. coli antibiotic susceptibility, and coexpression of Opa and the 3F11 LOS epitope mediated bacterial clumping (P <.01). In the human fallopian tube organ-culture model, Opa-producing variants attached up to 44-fold better than control bacteria (P <.01), and Por-producing variants exceeded submucosal invasion of control bacteria by 500-fold (P <.01). Opa and Por each facilitated intracellular invasion 20-40-fold (P <.01). In dual expresser variants, the 3F11 LOS epitope markedly reduced attachment and invasion mediated by Opa or Por. The LOS inhibitory effect was curbed when all 3 factors were expressed, which suggests an additional interaction of the 3 factors at the bacterial surface. Por, Opa, and LOS play important roles in Neisseria gonorrhoeae trafficking across human fallopian tube epithelium.
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U2 - 10.1086/322784
DO - 10.1086/322784
M3 - Article
C2 - 11471104
AN - SCOPUS:0035882505
VL - 184
SP - 460
EP - 472
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 4
ER -