Abstract
Intestinal infection by Cryptosporidium parvum causes inhibition of epithelial turnover, but underlying mechanisms are unclear. Previous studies demonstrate that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected epithelial cells. Using in vitro and in vivo models of intestinal cryptosporidiosis, we report here that host delivery of parasite Cdg7- FLc-1000 RNA results in inhibition of epithelial cell migration through suppression of the gene encoding sphingomyelinase 3 (SMPD3). Delivery of Cdg7-FLc-1000 into infected cells promotes the histone methyltransferase G9a-mediated H3K9 methylation in the SMPD3 locus. Te DNA-binding transcriptional repressor, PR domain zinc fnger protein 1, is required for the assembly of Cdg7-FLc-1000 into the G9a complex and associated with the enrichment of H3K9 methylation at the gene locus. Pathologically, nuclear transfer of Cryptosporidium parvum Cdg7-FLc-1000 RNA is involved in the attenuation of intestinal epithelial cell migration via trans-suppression of host cell SMPD3.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 122-133 |
| Number of pages | 12 |
| Journal | Journal of Infectious Diseases |
| Volume | 217 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2018 |
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All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Infectious Diseases
Cite this
Involvement of cryptosporidium parvum Cdg7-FLc-1000 RNA in the attenuation of intestinal epithelial cell migration via trans-suppression of host cell SMPD3. / Ming, Zhenping; Gong, Ai Yu; Wang, Yang; Zhang, Xin Tian; Li, Min; Mathy, Nicholas W.; Strauss-Soukup, Juliane K.; Chen, Xian-Ming.
In: Journal of Infectious Diseases, Vol. 217, No. 1, 01.01.2018, p. 122-133.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Involvement of cryptosporidium parvum Cdg7-FLc-1000 RNA in the attenuation of intestinal epithelial cell migration via trans-suppression of host cell SMPD3
AU - Ming, Zhenping
AU - Gong, Ai Yu
AU - Wang, Yang
AU - Zhang, Xin Tian
AU - Li, Min
AU - Mathy, Nicholas W.
AU - Strauss-Soukup, Juliane K.
AU - Chen, Xian-Ming
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Intestinal infection by Cryptosporidium parvum causes inhibition of epithelial turnover, but underlying mechanisms are unclear. Previous studies demonstrate that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected epithelial cells. Using in vitro and in vivo models of intestinal cryptosporidiosis, we report here that host delivery of parasite Cdg7- FLc-1000 RNA results in inhibition of epithelial cell migration through suppression of the gene encoding sphingomyelinase 3 (SMPD3). Delivery of Cdg7-FLc-1000 into infected cells promotes the histone methyltransferase G9a-mediated H3K9 methylation in the SMPD3 locus. Te DNA-binding transcriptional repressor, PR domain zinc fnger protein 1, is required for the assembly of Cdg7-FLc-1000 into the G9a complex and associated with the enrichment of H3K9 methylation at the gene locus. Pathologically, nuclear transfer of Cryptosporidium parvum Cdg7-FLc-1000 RNA is involved in the attenuation of intestinal epithelial cell migration via trans-suppression of host cell SMPD3.
AB - Intestinal infection by Cryptosporidium parvum causes inhibition of epithelial turnover, but underlying mechanisms are unclear. Previous studies demonstrate that a panel of parasite RNA transcripts of low protein-coding potential are delivered into infected epithelial cells. Using in vitro and in vivo models of intestinal cryptosporidiosis, we report here that host delivery of parasite Cdg7- FLc-1000 RNA results in inhibition of epithelial cell migration through suppression of the gene encoding sphingomyelinase 3 (SMPD3). Delivery of Cdg7-FLc-1000 into infected cells promotes the histone methyltransferase G9a-mediated H3K9 methylation in the SMPD3 locus. Te DNA-binding transcriptional repressor, PR domain zinc fnger protein 1, is required for the assembly of Cdg7-FLc-1000 into the G9a complex and associated with the enrichment of H3K9 methylation at the gene locus. Pathologically, nuclear transfer of Cryptosporidium parvum Cdg7-FLc-1000 RNA is involved in the attenuation of intestinal epithelial cell migration via trans-suppression of host cell SMPD3.
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UR - http://www.scopus.com/inward/citedby.url?scp=85042791991&partnerID=8YFLogxK
U2 - 10.1093/infdis/jix392
DO - 10.1093/infdis/jix392
M3 - Article
C2 - 28961856
AN - SCOPUS:85042791991
VL - 217
SP - 122
EP - 133
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 1
ER -