Is IGnRH-III the most potent GnRH analog containing only natural amino acids that specifically inhibits the growth of human breast cancer cells?

Krisztina Herédi-Szabó, Richard F. Murphy, Sándor Lovas

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Analogs of the decapeptide, gonadotropin-releasing hormone (GnRH), used in the treatment of hormone-dependent tumors, contain numerous unnatural amino acids, giving rise to many adverse effects. 1GnRH-III, a natural isoform of GnRH isolated from the sea lamprey, is a weak agonist of GnRH in the pituitary, but inhibits the growth of human cancer cells in micromolar concentrations. As 1GnRH-III is not a natural ligand in humans, it is possible that a more potent peptide, also containing only natural amino acids, can be synthesized. A positional scanning peptide library, focused on the variable region of the GnRH family of peptides, residues 5-8, was synthesized. The synthesized peptides were analyzed in competitive binding experiments and six new analogs were designed on the basis of the results. Their biological activities were evaluated in cell growth experiments. The only natural sequence selected was chicken GnRH-II. The synthetic library did not yield a more potent peptide than 1GnRH-III.

Original languageEnglish
Pages (from-to)714-720
Number of pages7
JournalJournal of Peptide Science
Volume12
Issue number11
DOIs
StatePublished - Nov 2006

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Gonadotropin-Releasing Hormone
Cells
Breast Neoplasms
Amino Acids
Growth
Peptides
Petromyzon
Peptide Library
Competitive Binding
Cell growth
Bioactivity
Libraries
Tumors
Chickens
Neoplasms
Protein Isoforms
Experiments
Hormones
Ligands
Scanning

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Is IGnRH-III the most potent GnRH analog containing only natural amino acids that specifically inhibits the growth of human breast cancer cells? / Herédi-Szabó, Krisztina; Murphy, Richard F.; Lovas, Sándor.

In: Journal of Peptide Science, Vol. 12, No. 11, 11.2006, p. 714-720.

Research output: Contribution to journalArticle

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