Islet secretory granules contain cytochrome b561

Robert Mackin, D. P. Jones, B. D. Noe

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A cytochrome has been detected in secretory granules prepared from anglerfish islets of Langerhans. The heme moiety was determined to be of the b type, and the dithionite-reduced cytochrome exhibited an α-band maximum at 561 nm with an extinction coefficient of 13.8 mM-1 · cm-1. The protein was present at a concentration of 40 ± 4 pmol/mg of secretory granule protein. The cytochrome was found to be an integral membrane protein and to be reduced by ascorbic acid but not by NADH, NADPH, reduced glutathione (GSH), or succinate. Because of the similarity to previously characterized secretory granule cytochrome b561's from neuroendocrine tissues, this cytochrome is also referred to as cytochrome b561. Although its function has not yet been elucidated, the apparent specificity for ascorbate suggests that it may be a component of the ascorbate-dependent peptidyl-glycine α-amidating monooxygenase system that functions in the amidation of islet hormones.

Original languageEnglish
Pages (from-to)881-885
Number of pages5
JournalDiabetes
Volume35
Issue number8
StatePublished - 1986
Externally publishedYes

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Secretory Vesicles
Cytochromes
Dithionite
Succinic Acid
Mixed Function Oxygenases
Heme
NADP
Islets of Langerhans
NAD
Glycine
Ascorbic Acid
Glutathione
Membrane Proteins
Proteins
Hormones
cytochrome b561

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Mackin, R., Jones, D. P., & Noe, B. D. (1986). Islet secretory granules contain cytochrome b561 . Diabetes, 35(8), 881-885.

Islet secretory granules contain cytochrome b561 . / Mackin, Robert; Jones, D. P.; Noe, B. D.

In: Diabetes, Vol. 35, No. 8, 1986, p. 881-885.

Research output: Contribution to journalArticle

Mackin, R, Jones, DP & Noe, BD 1986, 'Islet secretory granules contain cytochrome b561 ', Diabetes, vol. 35, no. 8, pp. 881-885.
Mackin, Robert ; Jones, D. P. ; Noe, B. D. / Islet secretory granules contain cytochrome b561 . In: Diabetes. 1986 ; Vol. 35, No. 8. pp. 881-885.
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