Islet secretory granules contain cytochrome b561

Robert Mackin, D. P. Jones, B. D. Noe

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Abstract

A cytochrome has been detected in secretory granules prepared from anglerfish islets of Langerhans. The heme moiety was determined to be of the b type, and the dithionite-reduced cytochrome exhibited an α-band maximum at 561 nm with an extinction coefficient of 13.8 mM-1 · cm-1. The protein was present at a concentration of 40 ± 4 pmol/mg of secretory granule protein. The cytochrome was found to be an integral membrane protein and to be reduced by ascorbic acid but not by NADH, NADPH, reduced glutathione (GSH), or succinate. Because of the similarity to previously characterized secretory granule cytochrome b561's from neuroendocrine tissues, this cytochrome is also referred to as cytochrome b561. Although its function has not yet been elucidated, the apparent specificity for ascorbate suggests that it may be a component of the ascorbate-dependent peptidyl-glycine α-amidating monooxygenase system that functions in the amidation of islet hormones.

Original languageEnglish
Pages (from-to)881-885
Number of pages5
JournalDiabetes
Volume35
Issue number8
Publication statusPublished - 1986
Externally publishedYes

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Mackin, R., Jones, D. P., & Noe, B. D. (1986). Islet secretory granules contain cytochrome b561 . Diabetes, 35(8), 881-885.