Abstract
The plasmid pCRG1600 is a 52.9-kb self-transmissible plasmid coding for resistance to aminoglycoside and β-lactam antibiotics in Staphylococcus aureus. When transferred by transduction, plasmid deletion mutants affecting one or more antibiotic-resistance genes were readily obtained. Of these, one derivative (pCRG1690) was found to exhibit a conjugal transfer frequency ca. 100-fold higher than that of the wild-type plasmid. A preliminary physical-genetic map of pCRG1600 located tra in a 14.6-kb region within the 16.9-kb XbaI-A fragment. An 8.5-kb deletion to the left of tra in pCRG1690 was specifically associated with the increased conjugal transferability of the plasmid. Thus, pCRG1690 appears similar to plasmids derepressed for conjugal transfer (drd) in gram-negative bacterial species.
| Original language | English |
|---|---|
| Pages (from-to) | 197-202 |
| Number of pages | 6 |
| Journal | Plasmid |
| Volume | 12 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1984 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
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Isolation and preliminary characterization of a plasmid mutant derepressed for conjugal transfer in Staphylococcus aureus. / Asch, D. K.; Goering, Richard V.; Ruff, E. A.
In: Plasmid, Vol. 12, No. 3, 1984, p. 197-202.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Isolation and preliminary characterization of a plasmid mutant derepressed for conjugal transfer in Staphylococcus aureus
AU - Asch, D. K.
AU - Goering, Richard V.
AU - Ruff, E. A.
PY - 1984
Y1 - 1984
N2 - The plasmid pCRG1600 is a 52.9-kb self-transmissible plasmid coding for resistance to aminoglycoside and β-lactam antibiotics in Staphylococcus aureus. When transferred by transduction, plasmid deletion mutants affecting one or more antibiotic-resistance genes were readily obtained. Of these, one derivative (pCRG1690) was found to exhibit a conjugal transfer frequency ca. 100-fold higher than that of the wild-type plasmid. A preliminary physical-genetic map of pCRG1600 located tra in a 14.6-kb region within the 16.9-kb XbaI-A fragment. An 8.5-kb deletion to the left of tra in pCRG1690 was specifically associated with the increased conjugal transferability of the plasmid. Thus, pCRG1690 appears similar to plasmids derepressed for conjugal transfer (drd) in gram-negative bacterial species.
AB - The plasmid pCRG1600 is a 52.9-kb self-transmissible plasmid coding for resistance to aminoglycoside and β-lactam antibiotics in Staphylococcus aureus. When transferred by transduction, plasmid deletion mutants affecting one or more antibiotic-resistance genes were readily obtained. Of these, one derivative (pCRG1690) was found to exhibit a conjugal transfer frequency ca. 100-fold higher than that of the wild-type plasmid. A preliminary physical-genetic map of pCRG1600 located tra in a 14.6-kb region within the 16.9-kb XbaI-A fragment. An 8.5-kb deletion to the left of tra in pCRG1690 was specifically associated with the increased conjugal transferability of the plasmid. Thus, pCRG1690 appears similar to plasmids derepressed for conjugal transfer (drd) in gram-negative bacterial species.
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U2 - 10.1016/0147-619X(84)90044-1
DO - 10.1016/0147-619X(84)90044-1
M3 - Article
VL - 12
SP - 197
EP - 202
JO - Plasmid
JF - Plasmid
SN - 0147-619X
IS - 3
ER -