Labeling of A1 adenosine receptors in porcine atria with the antagonist radioligand 8-cyclopentyl-1,3-[3H]dipropylxanthine

Mark Leid, Paul H. Franklin, Thomas F. Murray

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have used the antagonist radioligand 8-cyclopentyl-1,3-[3H]dipropylxanthine to label adenosine recognition sites in porcine atrial membranes. 8-Cyclopentyl-1,3-[3H]dipropylxanthine bound saturably, reversibly and with high affinity to an apparently homogenous population of recognition sites with a Bmax of 32.0 ± 0.9 fmol/mg protein and a KD of 0.394 ± 0.049 nM. Prototypic adenosine receptor agonists inhibited the specific binding of 8-cyclopentyl-1,3-[3H]dipropylxanthi in a manner consistent with the labeling of an A1 adenosine receptor. 8-Cyclopentyl-1,3-[3H]dipropylxanthine appears to be a valuable antagonist radioligand for the characterization of cardiac adenosine receptors.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
JournalEuropean Journal of Pharmacology
Volume147
Issue number1
DOIs
StatePublished - Feb 16 1988
Externally publishedYes

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Adenosine A1 Receptors
Swine
Purinergic P1 Receptor Agonists
Purinergic P1 Receptors
Adenosine
Membranes
Population
1,3-dipropyl-8-cyclopentylxanthine
Proteins

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Labeling of A1 adenosine receptors in porcine atria with the antagonist radioligand 8-cyclopentyl-1,3-[3H]dipropylxanthine. / Leid, Mark; Franklin, Paul H.; Murray, Thomas F.

In: European Journal of Pharmacology, Vol. 147, No. 1, 16.02.1988, p. 141-144.

Research output: Contribution to journalArticle

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