Abstract
We have used the antagonist radioligand 8-cyclopentyl-1,3-[3H]dipropylxanthine to label adenosine recognition sites in porcine atrial membranes. 8-Cyclopentyl-1,3-[3H]dipropylxanthine bound saturably, reversibly and with high affinity to an apparently homogenous population of recognition sites with a Bmax of 32.0 ± 0.9 fmol/mg protein and a KD of 0.394 ± 0.049 nM. Prototypic adenosine receptor agonists inhibited the specific binding of 8-cyclopentyl-1,3-[3H]dipropylxanthi in a manner consistent with the labeling of an A1 adenosine receptor. 8-Cyclopentyl-1,3-[3H]dipropylxanthine appears to be a valuable antagonist radioligand for the characterization of cardiac adenosine receptors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 141-144 |
| Number of pages | 4 |
| Journal | European Journal of Pharmacology |
| Volume | 147 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 16 1988 |
| Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Pharmacology
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Leid, M., Franklin, P. H., & Murray, T. F. (1988). Labeling of A1 adenosine receptors in porcine atria with the antagonist radioligand 8-cyclopentyl-1,3-[3H]dipropylxanthine. European Journal of Pharmacology, 147(1), 141-144. https://doi.org/10.1016/0014-2999(88)90644-9