TY - JOUR
T1 - Labeling of A1 adenosine receptors in porcine atria with the antagonist radioligand 8-cyclopentyl-1,3-[3H]dipropylxanthine
AU - Leid, Mark
AU - Franklin, Paul H.
AU - Murray, Thomas F.
PY - 1988/2/16
Y1 - 1988/2/16
N2 - We have used the antagonist radioligand 8-cyclopentyl-1,3-[3H]dipropylxanthine to label adenosine recognition sites in porcine atrial membranes. 8-Cyclopentyl-1,3-[3H]dipropylxanthine bound saturably, reversibly and with high affinity to an apparently homogenous population of recognition sites with a Bmax of 32.0 ± 0.9 fmol/mg protein and a KD of 0.394 ± 0.049 nM. Prototypic adenosine receptor agonists inhibited the specific binding of 8-cyclopentyl-1,3-[3H]dipropylxanthi in a manner consistent with the labeling of an A1 adenosine receptor. 8-Cyclopentyl-1,3-[3H]dipropylxanthine appears to be a valuable antagonist radioligand for the characterization of cardiac adenosine receptors.
AB - We have used the antagonist radioligand 8-cyclopentyl-1,3-[3H]dipropylxanthine to label adenosine recognition sites in porcine atrial membranes. 8-Cyclopentyl-1,3-[3H]dipropylxanthine bound saturably, reversibly and with high affinity to an apparently homogenous population of recognition sites with a Bmax of 32.0 ± 0.9 fmol/mg protein and a KD of 0.394 ± 0.049 nM. Prototypic adenosine receptor agonists inhibited the specific binding of 8-cyclopentyl-1,3-[3H]dipropylxanthi in a manner consistent with the labeling of an A1 adenosine receptor. 8-Cyclopentyl-1,3-[3H]dipropylxanthine appears to be a valuable antagonist radioligand for the characterization of cardiac adenosine receptors.
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U2 - 10.1016/0014-2999(88)90644-9
DO - 10.1016/0014-2999(88)90644-9
M3 - Article
C2 - 3371409
AN - SCOPUS:0023820527
VL - 147
SP - 141
EP - 144
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1
ER -