Ligand requirements for glmS ribozyme self-cleavage

Tom J. McCarthy, Melissa A. Plog, Shennen A. Floy, Joshua A. Jansen, Juliane K. Strauss-Soukup, Garrett Soukup

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Natural RNA catalysts (ribozymes) perform essential reactions in biological RNA processing and protein synthesis, whereby catalysis is intrinsic to RNA structure alone or in combination with metal ion cofactors. The recently discovered glmS ribozyme is unique in that it functions as a glucosamine-6-phosphate (GlcN6P)-dependent catalyst believed to enable "riboswitch" regulation of amino-sugar biosynthesis in certain prokaryotes. However, it is unclear whether GlcN6P functions as an effector or coenzyme to promote ribozyme self-cleavage. Herein, we demonstrate that ligand is absolutely requisite for glmS ribozyme self-cleavage activity. Furthermore, catalysis both requires and is dependent upon the acid dissociation constant (pKa) of the amine functionality of GlcN6P and related compounds. The data demonstrate that ligand is integral to catalysis, consistent with a coenzyme role for GlcN6P and illustrating an expanded capacity for biological RNA catalysis.

Original languageEnglish
Pages (from-to)1221-1226
Number of pages6
JournalChemistry and Biology
Volume12
Issue number11
DOIs
StatePublished - Nov 2005

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Catalytic RNA
Catalysis
RNA
Ligands
Coenzymes
Riboswitch
Amino Sugars
Catalysts
Biosynthesis
Amines
Metal ions
Metals
Ions
Acids
glucosamine 6-phosphate
Processing
Proteins

All Science Journal Classification (ASJC) codes

  • Organic Chemistry

Cite this

Ligand requirements for glmS ribozyme self-cleavage. / McCarthy, Tom J.; Plog, Melissa A.; Floy, Shennen A.; Jansen, Joshua A.; Strauss-Soukup, Juliane K.; Soukup, Garrett.

In: Chemistry and Biology, Vol. 12, No. 11, 11.2005, p. 1221-1226.

Research output: Contribution to journalArticle

McCarthy, Tom J. ; Plog, Melissa A. ; Floy, Shennen A. ; Jansen, Joshua A. ; Strauss-Soukup, Juliane K. ; Soukup, Garrett. / Ligand requirements for glmS ribozyme self-cleavage. In: Chemistry and Biology. 2005 ; Vol. 12, No. 11. pp. 1221-1226.
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