Liquid chromatographic method for the determination of nicotine in pharmaceutical formulations

Alekha K. Dash, Siew Tiing Wong

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


A simple high-performance liquid chromatography method was developed and validated for the analysis of nicotine in various pharmaceutical formulations. This method required a simple liquid-liquid extraction procedure prior to liquid chromatography analysis. The chromatographic separation was achieved on a reversed-phase C18 column with ultraviolet detection at 260 nm. This isocratic system was operated at ambient temperature and required 10 min of chromatographic time. The mobile phase consisted of methanol-citrate phosphate buffer (15:85, v/v) at a flow-rate of 0.7 ml/min. Standard curves were linear over the concentration range 1.0-51 μg/ml. Within-day and day-to-day relative standard deviations ranged from 1.3 to 4.4% and from 2.4 to 4.2%, respectively.

Original languageEnglish (US)
Pages (from-to)81-85
Number of pages5
JournalJournal of Chromatography A
Issue number1-2
StatePublished - Oct 18 1996

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


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