A simple high-performance liquid chromatography method was developed and validated for the analysis of nicotine in various pharmaceutical formulations. This method required a simple liquid-liquid extraction procedure prior to liquid chromatography analysis. The chromatographic separation was achieved on a reversed-phase C18 column with ultraviolet detection at 260 nm. This isocratic system was operated at ambient temperature and required 10 min of chromatographic time. The mobile phase consisted of methanol-citrate phosphate buffer (15:85, v/v) at a flow-rate of 0.7 ml/min. Standard curves were linear over the concentration range 1.0-51 μg/ml. Within-day and day-to-day relative standard deviations ranged from 1.3 to 4.4% and from 2.4 to 4.2%, respectively.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Organic Chemistry