Liquid chromatographic method for the determination of nicotine in pharmaceutical formulations

Alekha K. Dash, Siew Tiing Wong

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A simple high-performance liquid chromatography method was developed and validated for the analysis of nicotine in various pharmaceutical formulations. This method required a simple liquid-liquid extraction procedure prior to liquid chromatography analysis. The chromatographic separation was achieved on a reversed-phase C18 column with ultraviolet detection at 260 nm. This isocratic system was operated at ambient temperature and required 10 min of chromatographic time. The mobile phase consisted of methanol-citrate phosphate buffer (15:85, v/v) at a flow-rate of 0.7 ml/min. Standard curves were linear over the concentration range 1.0-51 μg/ml. Within-day and day-to-day relative standard deviations ranged from 1.3 to 4.4% and from 2.4 to 4.2%, respectively.

Original languageEnglish
Pages (from-to)81-85
Number of pages5
JournalJournal of Chromatography A
Volume749
Issue number1-2
DOIs
StatePublished - Oct 18 1996

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Drug Compounding
Nicotine
Liquid chromatography
High performance liquid chromatography
Liquids
Citric Acid
Pharmaceutical Preparations
Methanol
Buffers
Liquid-Liquid Extraction
Phosphates
Flow rate
Liquid Chromatography
High Pressure Liquid Chromatography
Temperature

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Liquid chromatographic method for the determination of nicotine in pharmaceutical formulations. / Dash, Alekha K.; Wong, Siew Tiing.

In: Journal of Chromatography A, Vol. 749, No. 1-2, 18.10.1996, p. 81-85.

Research output: Contribution to journalArticle

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