Liver microsomes contain multiple forms of inositol 1,4,5-trisphosphate binding proteins: Identification by nitrocellulose blot overlay

Nawab Ali, Devendra K. Agrawal

Research output: Contribution to journalArticlepeer-review

Abstract

A group of proteins binding to inositol 1,4,5-trisphosphate (IP3) has been identified in rat liver microsomes by a nitrocellulose blot-overlay technique. Proteins were resolved by SDS-PAGE, blotted on nitrocellulose and incubated with [32P]IP3 followed by autoradiography. Approximately eight IP3-binding polypeptides ranging Mr 23-50 kDA were present exclusively in microsomes; these were absent from plasma membrane and mitochondrial fractions. Binding of [32P]IP3 to these proteins was displaceable to a great extent by 5 μM unlabeled IP3 but not by 10 μM IP1, IP2, IP4, ATP, or GTPγS. These results suggest that liver microsomes contain multiple forms of IP3-binding proteins that can be detected by this new method.

Original languageEnglish
Pages (from-to)79-83
Number of pages5
JournalJournal of Pharmacological and Toxicological Methods
Volume27
Issue number2
DOIs
StatePublished - 1992

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Toxicology

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