Lizard and Frog Prestin

Evolutionary Insight into Functional Changes

Jie Tang, Jason L. Pecka, Bernd Fritzsch, Kirk Beisel, David Z. He

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The plasma membrane of mammalian cochlear outer hair cells contains prestin, a unique motor protein. Prestin is the fifth member of the solute carrier protein 26A family. Orthologs of prestin are also found in the ear of non-mammalian vertebrates such as zebrafish and chicken. However, these orthologs are electrogenic anion exchangers/transporters with no motor function. Amphibian and reptilian lineages represent phylogenic branches in the evolution of tetrapods and subsequent amniotes. Comparison of the peptide sequences and functional properties of these prestin orthologs offer new insights into prestin evolution. With the recent availability of the lizard and frog genome sequences, we examined amino acid sequence and function of lizard and frog prestins to determine how they are functionally and structurally different from prestins of mammals and other non-mammals. Somatic motility, voltage-dependent nonlinear capacitance (NLC), the two hallmarks of prestin function, and transport capability were measured in transfected human embryonic kidney cells using voltage-clamp and radioisotope techniques. We demonstrated that while the transport capability of lizard and frog prestin was compatible to that of chicken prestin, the NLC of lizard prestin was more robust than that of chicken's and was close to that of platypus. However, unlike platypus prestin which has acquired motor capability, lizard or frog prestin did not demonstrate motor capability. Lizard and frog prestins do not possess the same 11-amino-acid motif that is likely the structural adaptation for motor function in mammals. Thus, lizard and frog prestins appear to be functionally more advanced than that of chicken prestin, although motor capability is not yet acquired.

Original languageEnglish
Article numbere54388
JournalPLoS One
Volume8
Issue number1
DOIs
StatePublished - Jan 16 2013

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Lizards
Anura
lizards
frogs
Chickens
Platypus
Mammals
chickens
capacitance
Outer Auditory Hair Cells
Capacitance
Amino Acids
mammals
amino acid motifs
Clamping devices
Electric potential
antiporters
Amino Acid Motifs
Cell membranes
Radioisotopes

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lizard and Frog Prestin : Evolutionary Insight into Functional Changes. / Tang, Jie; Pecka, Jason L.; Fritzsch, Bernd; Beisel, Kirk; He, David Z.

In: PLoS One, Vol. 8, No. 1, e54388, 16.01.2013.

Research output: Contribution to journalArticle

Tang, Jie ; Pecka, Jason L. ; Fritzsch, Bernd ; Beisel, Kirk ; He, David Z. / Lizard and Frog Prestin : Evolutionary Insight into Functional Changes. In: PLoS One. 2013 ; Vol. 8, No. 1.
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