Abstract
Interleukin 3 (IL3), dependent cells were obtained from bone marrow (9/10 ex-periments) and spleen cells (4/5 experiments), but not from the thymus. These cclls were similar to mucosal mast cell toluidine blue staining and electron mi-croscopy. They had heterogenous metachromatic granules, and some had large scroll-like structures. They also contained histamine (200-800 ng/106 cells) for the first 2-5 weeks, whose level diminished to less than 30 ng/106 cells by 10 weeks of culture. They also generated leukotriene (LT) C4/D4 (10-40 ng/106 cclls) and LTB4 (2-5 ng/106 cells) for over 100 days of culture. In one experi-ment, bone marrow-derived mast cells after 150 days of culture began to pro-duce an IL3-like substance and proliferated exponentially without exogenous IL3.
Original language | English (US) |
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Pages (from-to) | 169-177 |
Number of pages | 9 |
Journal | International Archives of Allergy and Immunology |
Volume | 98 |
Issue number | 2 |
DOIs | |
State | Published - Jan 1 1992 |
All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology