Long-term cultured mouse mast cells: Ultrastructure, histamine and leukotriene levels

S. Haisa, H. S. Chang, A. K. Bewtra, M. Hiratani, N. Tamura, C. Bewtra, R. G. Townley

Research output: Contribution to journalArticle

6 Scopus citations


Interleukin 3 (IL3), dependent cells were obtained from bone marrow (9/10 ex-periments) and spleen cells (4/5 experiments), but not from the thymus. These cclls were similar to mucosal mast cell toluidine blue staining and electron mi-croscopy. They had heterogenous metachromatic granules, and some had large scroll-like structures. They also contained histamine (200-800 ng/106 cells) for the first 2-5 weeks, whose level diminished to less than 30 ng/106 cells by 10 weeks of culture. They also generated leukotriene (LT) C4/D4 (10-40 ng/106 cclls) and LTB4 (2-5 ng/106 cells) for over 100 days of culture. In one experi-ment, bone marrow-derived mast cells after 150 days of culture began to pro-duce an IL3-like substance and proliferated exponentially without exogenous IL3.

Original languageEnglish (US)
Pages (from-to)169-177
Number of pages9
JournalInternational Archives of Allergy and Immunology
Issue number2
StatePublished - Jan 1 1992


All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Haisa, S., Chang, H. S., Bewtra, A. K., Hiratani, M., Tamura, N., Bewtra, C., & Townley, R. G. (1992). Long-term cultured mouse mast cells: Ultrastructure, histamine and leukotriene levels. International Archives of Allergy and Immunology, 98(2), 169-177. https://doi.org/10.1159/000236181