Abstract
Interleukin 3 (IL3), dependent cells were obtained from bone marrow (9/10 ex-periments) and spleen cells (4/5 experiments), but not from the thymus. These cclls were similar to mucosal mast cell toluidine blue staining and electron mi-croscopy. They had heterogenous metachromatic granules, and some had large scroll-like structures. They also contained histamine (200-800 ng/106 cells) for the first 2-5 weeks, whose level diminished to less than 30 ng/106 cells by 10 weeks of culture. They also generated leukotriene (LT) C4/D4 (10-40 ng/106 cclls) and LTB4 (2-5 ng/106 cells) for over 100 days of culture. In one experi-ment, bone marrow-derived mast cells after 150 days of culture began to pro-duce an IL3-like substance and proliferated exponentially without exogenous IL3.
| Original language | English |
|---|---|
| Pages (from-to) | 169-177 |
| Number of pages | 9 |
| Journal | International Archives of Allergy and Immunology |
| Volume | 98 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1992 |
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All Science Journal Classification (ASJC) codes
- Immunology
- Immunology and Allergy
Cite this
Long-term cultured mouse mast cells : Ultrastructure, histamine and leukotriene levels. / Haisa, S.; Chang, H. S.; Bewtra, Againdra K.; Hiratani, M.; Tamura, N.; Bewtra, C.; Townley, R. G.
In: International Archives of Allergy and Immunology, Vol. 98, No. 2, 1992, p. 169-177.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Long-term cultured mouse mast cells
T2 - Ultrastructure, histamine and leukotriene levels
AU - Haisa, S.
AU - Chang, H. S.
AU - Bewtra, Againdra K.
AU - Hiratani, M.
AU - Tamura, N.
AU - Bewtra, C.
AU - Townley, R. G.
PY - 1992
Y1 - 1992
N2 - Interleukin 3 (IL3), dependent cells were obtained from bone marrow (9/10 ex-periments) and spleen cells (4/5 experiments), but not from the thymus. These cclls were similar to mucosal mast cell toluidine blue staining and electron mi-croscopy. They had heterogenous metachromatic granules, and some had large scroll-like structures. They also contained histamine (200-800 ng/106 cells) for the first 2-5 weeks, whose level diminished to less than 30 ng/106 cells by 10 weeks of culture. They also generated leukotriene (LT) C4/D4 (10-40 ng/106 cclls) and LTB4 (2-5 ng/106 cells) for over 100 days of culture. In one experi-ment, bone marrow-derived mast cells after 150 days of culture began to pro-duce an IL3-like substance and proliferated exponentially without exogenous IL3.
AB - Interleukin 3 (IL3), dependent cells were obtained from bone marrow (9/10 ex-periments) and spleen cells (4/5 experiments), but not from the thymus. These cclls were similar to mucosal mast cell toluidine blue staining and electron mi-croscopy. They had heterogenous metachromatic granules, and some had large scroll-like structures. They also contained histamine (200-800 ng/106 cells) for the first 2-5 weeks, whose level diminished to less than 30 ng/106 cells by 10 weeks of culture. They also generated leukotriene (LT) C4/D4 (10-40 ng/106 cclls) and LTB4 (2-5 ng/106 cells) for over 100 days of culture. In one experi-ment, bone marrow-derived mast cells after 150 days of culture began to pro-duce an IL3-like substance and proliferated exponentially without exogenous IL3.
UR - http://www.scopus.com/inward/record.url?scp=0026658951&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026658951&partnerID=8YFLogxK
U2 - 10.1159/000236181
DO - 10.1159/000236181
M3 - Article
C2 - 1643442
AN - SCOPUS:0026658951
VL - 98
SP - 169
EP - 177
JO - International Archives of Allergy and Immunology
JF - International Archives of Allergy and Immunology
SN - 1018-2438
IS - 2
ER -