Long-term cultured mouse mast cells

Ultrastructure, histamine and leukotriene levels

S. Haisa, H. S. Chang, Againdra K. Bewtra, M. Hiratani, N. Tamura, C. Bewtra, R. G. Townley

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Interleukin 3 (IL3), dependent cells were obtained from bone marrow (9/10 ex-periments) and spleen cells (4/5 experiments), but not from the thymus. These cclls were similar to mucosal mast cell toluidine blue staining and electron mi-croscopy. They had heterogenous metachromatic granules, and some had large scroll-like structures. They also contained histamine (200-800 ng/106 cells) for the first 2-5 weeks, whose level diminished to less than 30 ng/106 cells by 10 weeks of culture. They also generated leukotriene (LT) C4/D4 (10-40 ng/106 cclls) and LTB4 (2-5 ng/106 cells) for over 100 days of culture. In one experi-ment, bone marrow-derived mast cells after 150 days of culture began to pro-duce an IL3-like substance and proliferated exponentially without exogenous IL3.

Original languageEnglish
Pages (from-to)169-177
Number of pages9
JournalInternational Archives of Allergy and Immunology
Volume98
Issue number2
DOIs
StatePublished - 1992

Fingerprint

Leukotrienes
Mast Cells
Histamine
Interleukin-3
Bone Marrow
Leukotriene D4
Leukotriene C4
Tolonium Chloride
Leukotriene B4
Thymus Gland
Spleen
Electrons
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Immunology
  • Immunology and Allergy

Cite this

Long-term cultured mouse mast cells : Ultrastructure, histamine and leukotriene levels. / Haisa, S.; Chang, H. S.; Bewtra, Againdra K.; Hiratani, M.; Tamura, N.; Bewtra, C.; Townley, R. G.

In: International Archives of Allergy and Immunology, Vol. 98, No. 2, 1992, p. 169-177.

Research output: Contribution to journalArticle

Haisa, S. ; Chang, H. S. ; Bewtra, Againdra K. ; Hiratani, M. ; Tamura, N. ; Bewtra, C. ; Townley, R. G. / Long-term cultured mouse mast cells : Ultrastructure, histamine and leukotriene levels. In: International Archives of Allergy and Immunology. 1992 ; Vol. 98, No. 2. pp. 169-177.
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AU - Bewtra, C.

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