Measurement of bronchoconstriction using whole-body plethysmograph: Comparison of freely moving versus restrained guinea pigs

B. T Y Chong, Devendra K. Agrawal, F. A. Romero, R. G. Townley

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

We have previously measured pulmonary function in guinea pigs using a double-chambered plethysmograph, however, the question remains regarding the accuracy of the double-chamber to gauge the long-term pulmonary function of late asthmatic response. This may be affected by confounding factors, such as stress on the animal and differences in size of the collar around the neck. Therefore, in this study we compared histamine-induced bronchoconstriction in the same guinea pigs using a single- versus a double-chambered body box. In the double-chambered body box, the specific airway resistance is proportional to time delay between thoracic and nasal flows and measured in cmH2O . s. Whereas, in the single-chambered body box, PenH units (Enhanced Pause) reflect 'effort of breathing.' This is measured as the pause between inspiration and expiration. Doubling concentrations of histamine (12.5-200 μg/ml dissolved in normal saline) were administered by DeVilbiss nebulizer for 1 min, followed by 1 min suction of residual drug in the chamber, and then the airway resistance was recorded by the computer for the following 3 min. There was a 15-min wash-out period between two doses of histamine. There was no statistically significant difference (p > 0.05) in the PC100 values for histamine between the two methods, however, it was much easier to work with the single-chambered body box in terms of handling the animal and eliminating the possible influence of collar placement on the bronchoconstriction. In conclusion, the data suggests histamine challenges produce equivalent PC100 data in both the double-chambered plethysmograph with sRAW units and single-chambered plethysmograph using the PenH units. Copyright (C) 1998 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalJournal of Pharmacological and Toxicological Methods
Volume39
Issue number3
DOIs
StatePublished - Apr 1998

Fingerprint

Bronchoconstriction
Histamine
Guinea Pigs
Airway Resistance
Animals
Lung
Nebulizers and Vaporizers
Suction
Nose
Gages
Time delay
Respiration
Neck
Thorax
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Toxicology

Cite this

Measurement of bronchoconstriction using whole-body plethysmograph : Comparison of freely moving versus restrained guinea pigs. / Chong, B. T Y; Agrawal, Devendra K.; Romero, F. A.; Townley, R. G.

In: Journal of Pharmacological and Toxicological Methods, Vol. 39, No. 3, 04.1998, p. 163-168.

Research output: Contribution to journalArticle

@article{c8501bd36e704ee49c4608754c2c5fbe,
title = "Measurement of bronchoconstriction using whole-body plethysmograph: Comparison of freely moving versus restrained guinea pigs",
abstract = "We have previously measured pulmonary function in guinea pigs using a double-chambered plethysmograph, however, the question remains regarding the accuracy of the double-chamber to gauge the long-term pulmonary function of late asthmatic response. This may be affected by confounding factors, such as stress on the animal and differences in size of the collar around the neck. Therefore, in this study we compared histamine-induced bronchoconstriction in the same guinea pigs using a single- versus a double-chambered body box. In the double-chambered body box, the specific airway resistance is proportional to time delay between thoracic and nasal flows and measured in cmH2O . s. Whereas, in the single-chambered body box, PenH units (Enhanced Pause) reflect 'effort of breathing.' This is measured as the pause between inspiration and expiration. Doubling concentrations of histamine (12.5-200 μg/ml dissolved in normal saline) were administered by DeVilbiss nebulizer for 1 min, followed by 1 min suction of residual drug in the chamber, and then the airway resistance was recorded by the computer for the following 3 min. There was a 15-min wash-out period between two doses of histamine. There was no statistically significant difference (p > 0.05) in the PC100 values for histamine between the two methods, however, it was much easier to work with the single-chambered body box in terms of handling the animal and eliminating the possible influence of collar placement on the bronchoconstriction. In conclusion, the data suggests histamine challenges produce equivalent PC100 data in both the double-chambered plethysmograph with sRAW units and single-chambered plethysmograph using the PenH units. Copyright (C) 1998 Elsevier Science Inc.",
author = "Chong, {B. T Y} and Agrawal, {Devendra K.} and Romero, {F. A.} and Townley, {R. G.}",
year = "1998",
month = "4",
doi = "10.1016/S1056-8719(98)00021-5",
language = "English",
volume = "39",
pages = "163--168",
journal = "Journal of Pharmacological and Toxicological Methods",
issn = "1056-8719",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Measurement of bronchoconstriction using whole-body plethysmograph

T2 - Comparison of freely moving versus restrained guinea pigs

AU - Chong, B. T Y

AU - Agrawal, Devendra K.

AU - Romero, F. A.

AU - Townley, R. G.

PY - 1998/4

Y1 - 1998/4

N2 - We have previously measured pulmonary function in guinea pigs using a double-chambered plethysmograph, however, the question remains regarding the accuracy of the double-chamber to gauge the long-term pulmonary function of late asthmatic response. This may be affected by confounding factors, such as stress on the animal and differences in size of the collar around the neck. Therefore, in this study we compared histamine-induced bronchoconstriction in the same guinea pigs using a single- versus a double-chambered body box. In the double-chambered body box, the specific airway resistance is proportional to time delay between thoracic and nasal flows and measured in cmH2O . s. Whereas, in the single-chambered body box, PenH units (Enhanced Pause) reflect 'effort of breathing.' This is measured as the pause between inspiration and expiration. Doubling concentrations of histamine (12.5-200 μg/ml dissolved in normal saline) were administered by DeVilbiss nebulizer for 1 min, followed by 1 min suction of residual drug in the chamber, and then the airway resistance was recorded by the computer for the following 3 min. There was a 15-min wash-out period between two doses of histamine. There was no statistically significant difference (p > 0.05) in the PC100 values for histamine between the two methods, however, it was much easier to work with the single-chambered body box in terms of handling the animal and eliminating the possible influence of collar placement on the bronchoconstriction. In conclusion, the data suggests histamine challenges produce equivalent PC100 data in both the double-chambered plethysmograph with sRAW units and single-chambered plethysmograph using the PenH units. Copyright (C) 1998 Elsevier Science Inc.

AB - We have previously measured pulmonary function in guinea pigs using a double-chambered plethysmograph, however, the question remains regarding the accuracy of the double-chamber to gauge the long-term pulmonary function of late asthmatic response. This may be affected by confounding factors, such as stress on the animal and differences in size of the collar around the neck. Therefore, in this study we compared histamine-induced bronchoconstriction in the same guinea pigs using a single- versus a double-chambered body box. In the double-chambered body box, the specific airway resistance is proportional to time delay between thoracic and nasal flows and measured in cmH2O . s. Whereas, in the single-chambered body box, PenH units (Enhanced Pause) reflect 'effort of breathing.' This is measured as the pause between inspiration and expiration. Doubling concentrations of histamine (12.5-200 μg/ml dissolved in normal saline) were administered by DeVilbiss nebulizer for 1 min, followed by 1 min suction of residual drug in the chamber, and then the airway resistance was recorded by the computer for the following 3 min. There was a 15-min wash-out period between two doses of histamine. There was no statistically significant difference (p > 0.05) in the PC100 values for histamine between the two methods, however, it was much easier to work with the single-chambered body box in terms of handling the animal and eliminating the possible influence of collar placement on the bronchoconstriction. In conclusion, the data suggests histamine challenges produce equivalent PC100 data in both the double-chambered plethysmograph with sRAW units and single-chambered plethysmograph using the PenH units. Copyright (C) 1998 Elsevier Science Inc.

UR - http://www.scopus.com/inward/record.url?scp=0032047333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032047333&partnerID=8YFLogxK

U2 - 10.1016/S1056-8719(98)00021-5

DO - 10.1016/S1056-8719(98)00021-5

M3 - Article

C2 - 9741391

AN - SCOPUS:0032047333

VL - 39

SP - 163

EP - 168

JO - Journal of Pharmacological and Toxicological Methods

JF - Journal of Pharmacological and Toxicological Methods

SN - 1056-8719

IS - 3

ER -