Measurement of serum isopropanol and the acetone metabolite by proton nuclear magnetic resonance

Application to pharmacokinetic evaluation in a simulated overdose model

Michael S. Monaghan, Keith M. Olsen, Bruce H. Ackerman, Gary L. Fuller, William H. Porter, Alex A. Pappas

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The purpose of this investigation was to 1) compare the performance of proton nuclear magnetic resonance spectroscopy to gas chromatography head-space analysis in the measurement of serum isopropanol and its metabolite, acetone, obtained during a simulated overdose, and 2) compare pharmacokinetic parameters obtained using the two analytical techniques. Three healthy volunteers ingested 0.6 mL/kg of 70 % isopropanol and blood samples were obtained at baseline, 0.16, 0.33, 0.66, 1.0, 1.5, 2.0, 3.0, 4.0, 6.0, 8.0, 12.0, and 24.0 hours post-ingestion. Resulting sera were analyzed by gas chromatography head-space analysis and proton nuclear magnetic resonance spectroscopy for determination of isopropanol and acetone concentrations. A correlation between concentrations quantitated by gas chromatography head-space analysis versus proton nuclear magnetic resonance spectroscopy was determined using linear regression. Pharmacokinetic disposition parameters were determined from serum concentration-time data and compared using analysis of variance. For isopropanol, the linear regression equation which describes the relationship between gas chromatography head-space analysis and proton nuclear magnetic resonance spectroscopy was y = 1.041x - 2.180 (r2 = 0.995, p <0.0001); for acetone, y = 1.022x - 0.946 (r2 = 0.984, p <0.0001). Pharmacokinetic disposition parameters derived from the two analytical methods were comparable. Proton nuclear magnetic resonance spectroscopy can be used to rapidly quantitate serum isopropanol and acetone concentrations in the same sample when gas chromatography head-space analysis is unavailable. Also, proton nuclear magnetic resonance spectroscopy can be used to follow serial serum concentrations during an ingestion for the purpose of pharmacokinetic analysis.

Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalClinical Toxicology
Volume33
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Pharmacokinetics
2-Propanol
Metabolites
Acetone
Nuclear magnetic resonance spectroscopy
Protons
Magnetic Resonance Spectroscopy
Gas chromatography
Gas Chromatography
Nuclear magnetic resonance
Head
Serum
Linear regression
Linear Models
Eating
Analysis of variance (ANOVA)
Proton Magnetic Resonance Spectroscopy
Analysis of Variance
Healthy Volunteers
Blood

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Measurement of serum isopropanol and the acetone metabolite by proton nuclear magnetic resonance : Application to pharmacokinetic evaluation in a simulated overdose model. / Monaghan, Michael S.; Olsen, Keith M.; Ackerman, Bruce H.; Fuller, Gary L.; Porter, William H.; Pappas, Alex A.

In: Clinical Toxicology, Vol. 33, No. 2, 1995, p. 141-149.

Research output: Contribution to journalArticle

Monaghan, Michael S. ; Olsen, Keith M. ; Ackerman, Bruce H. ; Fuller, Gary L. ; Porter, William H. ; Pappas, Alex A. / Measurement of serum isopropanol and the acetone metabolite by proton nuclear magnetic resonance : Application to pharmacokinetic evaluation in a simulated overdose model. In: Clinical Toxicology. 1995 ; Vol. 33, No. 2. pp. 141-149.
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AB - The purpose of this investigation was to 1) compare the performance of proton nuclear magnetic resonance spectroscopy to gas chromatography head-space analysis in the measurement of serum isopropanol and its metabolite, acetone, obtained during a simulated overdose, and 2) compare pharmacokinetic parameters obtained using the two analytical techniques. Three healthy volunteers ingested 0.6 mL/kg of 70 % isopropanol and blood samples were obtained at baseline, 0.16, 0.33, 0.66, 1.0, 1.5, 2.0, 3.0, 4.0, 6.0, 8.0, 12.0, and 24.0 hours post-ingestion. Resulting sera were analyzed by gas chromatography head-space analysis and proton nuclear magnetic resonance spectroscopy for determination of isopropanol and acetone concentrations. A correlation between concentrations quantitated by gas chromatography head-space analysis versus proton nuclear magnetic resonance spectroscopy was determined using linear regression. Pharmacokinetic disposition parameters were determined from serum concentration-time data and compared using analysis of variance. For isopropanol, the linear regression equation which describes the relationship between gas chromatography head-space analysis and proton nuclear magnetic resonance spectroscopy was y = 1.041x - 2.180 (r2 = 0.995, p <0.0001); for acetone, y = 1.022x - 0.946 (r2 = 0.984, p <0.0001). Pharmacokinetic disposition parameters derived from the two analytical methods were comparable. Proton nuclear magnetic resonance spectroscopy can be used to rapidly quantitate serum isopropanol and acetone concentrations in the same sample when gas chromatography head-space analysis is unavailable. Also, proton nuclear magnetic resonance spectroscopy can be used to follow serial serum concentrations during an ingestion for the purpose of pharmacokinetic analysis.

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