TY - JOUR
T1 - Metabolic imaging of the organ of corti - A window on cochlea bioenergetics
AU - Tiede, Le Ann
AU - Steyger, Peter S.
AU - Nichols, Michael G.
AU - Hallworth, Richard J.
N1 - Funding Information:
Funding for this research was received from N.I.H. R21 DC008995 and the American Hearing Research Foundation. This research was conducted at the Integrative Biological Imaging Facility at Creighton University School of Medicine. This facility was constructed with support from C06 Grant RR17417-01 from the N.C.R.R., N.I.H.
PY - 2009/6/24
Y1 - 2009/6/24
N2 - Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.
AB - Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.
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U2 - 10.1016/j.brainres.2009.02.052
DO - 10.1016/j.brainres.2009.02.052
M3 - Article
C2 - 19272358
AN - SCOPUS:67649544483
VL - 1277
SP - 37
EP - 41
JO - Brain Research
JF - Brain Research
SN - 0006-8993
ER -