Abstract
Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.
| Original language | English |
|---|---|
| Pages (from-to) | 37-41 |
| Number of pages | 5 |
| Journal | Brain Research |
| Volume | 1277 |
| DOIs | |
| State | Published - Jun 24 2009 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Clinical Neurology
- Developmental Biology
- Molecular Biology
Cite this
Metabolic imaging of the organ of corti - A window on cochlea bioenergetics. / Tiede, Le Ann; Steyger, Peter S.; Nichols, Michael G.; Hallworth, Richard J.
In: Brain Research, Vol. 1277, 24.06.2009, p. 37-41.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Metabolic imaging of the organ of corti - A window on cochlea bioenergetics
AU - Tiede, Le Ann
AU - Steyger, Peter S.
AU - Nichols, Michael G.
AU - Hallworth, Richard J.
PY - 2009/6/24
Y1 - 2009/6/24
N2 - Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.
AB - Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.
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UR - http://www.scopus.com/inward/citedby.url?scp=67649544483&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2009.02.052
DO - 10.1016/j.brainres.2009.02.052
M3 - Article
VL - 1277
SP - 37
EP - 41
JO - Brain Research
JF - Brain Research
SN - 0006-8993
ER -