Metabolic imaging of the organ of corti - A window on cochlea bioenergetics

Le Ann Tiede, Peter S. Steyger, Michael G. Nichols, Richard J. Hallworth

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.

Original languageEnglish
Pages (from-to)37-41
Number of pages5
JournalBrain Research
Volume1277
DOIs
StatePublished - Jun 24 2009

Fingerprint

Organ of Corti
Cochlea
Energy Metabolism
NAD
Alopecia
Aminoglycosides
Outer Auditory Hair Cells
Inner Auditory Hair Cells
Noise-Induced Hearing Loss
Sensorineural Hearing Loss
Gentamicins
Photons
Mitochondria

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Metabolic imaging of the organ of corti - A window on cochlea bioenergetics. / Tiede, Le Ann; Steyger, Peter S.; Nichols, Michael G.; Hallworth, Richard J.

In: Brain Research, Vol. 1277, 24.06.2009, p. 37-41.

Research output: Contribution to journalArticle

Tiede, Le Ann ; Steyger, Peter S. ; Nichols, Michael G. ; Hallworth, Richard J. / Metabolic imaging of the organ of corti - A window on cochlea bioenergetics. In: Brain Research. 2009 ; Vol. 1277. pp. 37-41.
@article{74b30ca960be4255b609981d0e711d58,
title = "Metabolic imaging of the organ of corti - A window on cochlea bioenergetics",
abstract = "Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.",
author = "Tiede, {Le Ann} and Steyger, {Peter S.} and Nichols, {Michael G.} and Hallworth, {Richard J.}",
year = "2009",
month = "6",
day = "24",
doi = "10.1016/j.brainres.2009.02.052",
language = "English",
volume = "1277",
pages = "37--41",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",

}

TY - JOUR

T1 - Metabolic imaging of the organ of corti - A window on cochlea bioenergetics

AU - Tiede, Le Ann

AU - Steyger, Peter S.

AU - Nichols, Michael G.

AU - Hallworth, Richard J.

PY - 2009/6/24

Y1 - 2009/6/24

N2 - Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.

AB - Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.

UR - http://www.scopus.com/inward/record.url?scp=67649544483&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649544483&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2009.02.052

DO - 10.1016/j.brainres.2009.02.052

M3 - Article

C2 - 19272358

AN - SCOPUS:67649544483

VL - 1277

SP - 37

EP - 41

JO - Brain Research

JF - Brain Research

SN - 0006-8993

ER -