Metabolic imaging of the organ of corti - A window on cochlea bioenergetics

Le Ann Tiede; Peter S. Steyger; Michael G. Nichols; Richard Hallworth

doi:10.1016/j.brainres.2009.02.052

Metabolic imaging of the organ of corti - A window on cochlea bioenergetics

Le Ann Tiede, Peter S. Steyger, Michael G. Nichols, Richard Hallworth

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20 Scopus citations

Abstract

Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.

Original language	English (US)
Pages (from-to)	37-41
Number of pages	5
Journal	Brain Research
Volume	1277
DOIs	https://doi.org/10.1016/j.brainres.2009.02.052
State	Published - Jun 24 2009

All Science Journal Classification (ASJC) codes

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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title = "Metabolic imaging of the organ of corti - A window on cochlea bioenergetics",

abstract = "Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.",

author = "Tiede, {Le Ann} and Steyger, {Peter S.} and Nichols, {Michael G.} and Richard Hallworth",

note = "Funding Information: Funding for this research was received from N.I.H. R21 DC008995 and the American Hearing Research Foundation. This research was conducted at the Integrative Biological Imaging Facility at Creighton University School of Medicine. This facility was constructed with support from C06 Grant RR17417-01 from the N.C.R.R., N.I.H. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",

year = "2009",

month = jun,

day = "24",

doi = "10.1016/j.brainres.2009.02.052",

language = "English (US)",

volume = "1277",

pages = "37--41",

journal = "Brain Research",

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AU - Tiede, Le Ann

AU - Steyger, Peter S.

AU - Nichols, Michael G.

AU - Hallworth, Richard

N1 - Funding Information: Funding for this research was received from N.I.H. R21 DC008995 and the American Hearing Research Foundation. This research was conducted at the Integrative Biological Imaging Facility at Creighton University School of Medicine. This facility was constructed with support from C06 Grant RR17417-01 from the N.C.R.R., N.I.H. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.

PY - 2009/6/24

Y1 - 2009/6/24

N2 - Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.

AB - Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.

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Metabolic imaging of the organ of corti - A window on cochlea bioenergetics

Abstract

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