Metabolic imaging using two-photon excited nadh intensity and fluorescence lifetime imaging

Jorge Vergen, Clifford Hecht, Lyandysha V. Zholudeva, Meg M. Marquardt, Richard J. Hallworth, Michael G. Nichols

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Metabolism and mitochondrial dysfunction are known to be involved in many different disease states. We have employed two-photon fluorescence imaging of intrinsic mitochondrial reduced nicotinamide adenine dinucleotide (NADH) to quantify the metabolic state of several cultured cell lines, multicell tumor spheroids, and the intact mouse organ of Corti. Historically, fluorescence intensity has commonly been used as an indicator of the NADH concentration in cells and tissues. More recently, fluorescence lifetime imaging has revealed that changes in metabolism produce not only changes in fluorescence intensity, but also significant changes in the lifetimes and concentrations of free and enzyme-bound pools of NADH. Since NADH binding changes with metabolic state, this approach presents a new opportunity to track the cellular metabolic state.

Original languageEnglish
Pages (from-to)761-770
Number of pages10
JournalMicroscopy and Microanalysis
Volume18
Issue number4
DOIs
StatePublished - Aug 2012

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Photons
Fluorescence
Imaging techniques
life (durability)
fluorescence
photons
metabolism
cultured cells
Metabolism
nicotinamide
spheroids
adenines
organs
mice
enzymes
Tumors
tumors
Enzymes
Cells
Tissue

All Science Journal Classification (ASJC) codes

  • Instrumentation

Cite this

Metabolic imaging using two-photon excited nadh intensity and fluorescence lifetime imaging. / Vergen, Jorge; Hecht, Clifford; Zholudeva, Lyandysha V.; Marquardt, Meg M.; Hallworth, Richard J.; Nichols, Michael G.

In: Microscopy and Microanalysis, Vol. 18, No. 4, 08.2012, p. 761-770.

Research output: Contribution to journalArticle

Vergen, Jorge ; Hecht, Clifford ; Zholudeva, Lyandysha V. ; Marquardt, Meg M. ; Hallworth, Richard J. ; Nichols, Michael G. / Metabolic imaging using two-photon excited nadh intensity and fluorescence lifetime imaging. In: Microscopy and Microanalysis. 2012 ; Vol. 18, No. 4. pp. 761-770.
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