Methods for quantifying gene expression in ecoimmunology

From qPCR to RNA-Seq

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Synopsis Historically, the use of cutting-edge molecular techniques to study immunological gene expression and related cellular pathways has been largely limited to model organisms. Few studies have been performed that quantify the molecular immunological responses of non-model species, especially in response to environmental factors, life-history events, or exposure to parasites. This dearth of information has largely occurred due to the lack of available non-model species-specific gene sequences and immunological reagents and also due to prohibitively expensive technology. However, with the rapid development of various sequencing and transcriptomic technologies, profiling the gene expression of nonmodel organisms has become possible. Technologies and concepts explored here include an overview of current technologies for quantifying gene expression, including: qPCR, multiplex branched DNA assays, microarrays, and profiling gene expression (RNA sequencing [RNA-Seq]) based on next-generation sequencing. Examples of the advancement of these technologies in non-model systems are discussed. Additionally, applications, limitations, and feasibility of the use of these methodologies in non-model systems to address questions in ecological immunology and disease-ecology are specifically addressed.

Original languageEnglish
Pages (from-to)396-406
Number of pages11
JournalIntegrative and Comparative Biology
Volume54
Issue number3
DOIs
StatePublished - 2014

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RNA Sequence Analysis
sequence analysis
Technology
Gene Expression
gene expression
Branched DNA Signal Amplification Assay
methodology
DNA Fingerprinting
organisms
Gene Expression Profiling
Allergy and Immunology
Ecology
Oligonucleotide Array Sequence Analysis
transcriptomics
immunology
Parasites
life history
immune response
ecology
nucleotide sequences

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Plant Science
  • Medicine(all)

Cite this

Methods for quantifying gene expression in ecoimmunology : From qPCR to RNA-Seq. / Fassbinder-Orth, Carol.

In: Integrative and Comparative Biology, Vol. 54, No. 3, 2014, p. 396-406.

Research output: Contribution to journalArticle

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