Mice lacking the folic acid-binding protein Folbp1 are defective in early embryonic development

Jorge A. Piedrahita, Betty Oetama, Gregory D. Bennett, Janee Gelineau-van Waes, Barton A. Kamen, James Richardson, Stephen W. Lacey, Richard G W Anderson, Richard H. Finnell

Research output: Contribution to journalArticle

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Abstract

Periconceptional folic acid supplementation reduces the occurrence of several human congenital malformations, including craniofacial, heart and neural tube defects. Although the underlying mechanism is unknown, there may be a maternal-to-fetal folate-transport defect or an inherent fetal biochemical disorder that is neutralized by supplementation. Previous experiments have identified a folate-binding protein (Folbp1) that functions as a membrane receptor to mediate the high-affinity internalization and delivery of folate to the cytoplasm of the cell. In vitro, this receptor facilitates the accumulation of cellular folate a thousand-fold relative to the media, suggesting that it may be essential in cytoplasmic folate delivery in vivo. The importance of an adequate intracellular folate pool for normal embryogenesis has long been recognized in humans and experimental animals. To determine whether Folbp1 is involved in maternal-to-fetal folate transport, we inactivated Folbp1 in mice. We also produced mice lacking Folbp2, another member of the folate receptor family that is GPI anchored but binds folate poorly. Folbp2(-/-) embryos developed normally, but Folbp1(-/-) embryos had severe morphogenetic abnormalities and died in utero by embryonic day (E) 10. Supplementing pregnant Folbp1(+/-) dams with folinic acid reversed this phenotype in nullizygous pups. Our results suggest that Folbp1 has a critical role in folate homeostasis during development, and that functional defects in the human homologue (FOLR1) of Folbp1 may contribute to similar defects in humans.

Original languageEnglish
Pages (from-to)228-232
Number of pages5
JournalNature Genetics
Volume23
Issue number2
DOIs
StatePublished - Oct 1999
Externally publishedYes

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Folic Acid
Embryonic Development
Carrier Proteins
Embryonic Structures
Mothers
Leucovorin
Neural Tube Defects
Cytoplasm
Homeostasis
Phenotype
Membranes

All Science Journal Classification (ASJC) codes

  • Genetics(clinical)
  • Genetics

Cite this

Piedrahita, J. A., Oetama, B., Bennett, G. D., Gelineau-van Waes, J., Kamen, B. A., Richardson, J., ... Finnell, R. H. (1999). Mice lacking the folic acid-binding protein Folbp1 are defective in early embryonic development. Nature Genetics, 23(2), 228-232. https://doi.org/10.1038/13861

Mice lacking the folic acid-binding protein Folbp1 are defective in early embryonic development. / Piedrahita, Jorge A.; Oetama, Betty; Bennett, Gregory D.; Gelineau-van Waes, Janee; Kamen, Barton A.; Richardson, James; Lacey, Stephen W.; Anderson, Richard G W; Finnell, Richard H.

In: Nature Genetics, Vol. 23, No. 2, 10.1999, p. 228-232.

Research output: Contribution to journalArticle

Piedrahita, JA, Oetama, B, Bennett, GD, Gelineau-van Waes, J, Kamen, BA, Richardson, J, Lacey, SW, Anderson, RGW & Finnell, RH 1999, 'Mice lacking the folic acid-binding protein Folbp1 are defective in early embryonic development', Nature Genetics, vol. 23, no. 2, pp. 228-232. https://doi.org/10.1038/13861
Piedrahita, Jorge A. ; Oetama, Betty ; Bennett, Gregory D. ; Gelineau-van Waes, Janee ; Kamen, Barton A. ; Richardson, James ; Lacey, Stephen W. ; Anderson, Richard G W ; Finnell, Richard H. / Mice lacking the folic acid-binding protein Folbp1 are defective in early embryonic development. In: Nature Genetics. 1999 ; Vol. 23, No. 2. pp. 228-232.
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