Microbial typing by matrix-assisted laser desorption ionization-time of flight mass spectrometry: Do we need guidance for data interpretation?

Sébastien Spinali, Alex Van Belkum, Richard V. Goering, Victoria Girard, Martin Welker, Marc Van Nuenen, David H. Pincus, Maud Arsac, Géraldine Durand

Research output: Contribution to journalReview article

54 Citations (Scopus)

Abstract

The integration of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) in clinical microbiology has revolutionized species identification of bacteria, yeasts, and molds. However, beyond straightforward identification, the method has also been suggested to have the potential for subspecies-level or even type-level epidemiological analyses. This minireview explores MALDI-TOF MS-based typing, which has already been performed on many clinically relevant species. We discuss the limits of the method's resolution and we suggest interpretative criteria allowing valid comparison of strain-specific data. We conclude that guidelines for MALDI-TOF MS-based typing can be developed along the same lines as those used for the interpretation of data from pulsed-field gel electrophoresis (PFGE).

Original languageEnglish
Pages (from-to)760-765
Number of pages6
JournalJournal of Clinical Microbiology
Volume53
Issue number3
DOIs
StatePublished - Mar 1 2015

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Mass Spectrometry
Lasers
Pulsed Field Gel Electrophoresis
Microbiology
Fungi
Yeasts
Guidelines
Bacteria

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Microbial typing by matrix-assisted laser desorption ionization-time of flight mass spectrometry : Do we need guidance for data interpretation? / Spinali, Sébastien; Van Belkum, Alex; Goering, Richard V.; Girard, Victoria; Welker, Martin; Van Nuenen, Marc; Pincus, David H.; Arsac, Maud; Durand, Géraldine.

In: Journal of Clinical Microbiology, Vol. 53, No. 3, 01.03.2015, p. 760-765.

Research output: Contribution to journalReview article

Spinali, Sébastien ; Van Belkum, Alex ; Goering, Richard V. ; Girard, Victoria ; Welker, Martin ; Van Nuenen, Marc ; Pincus, David H. ; Arsac, Maud ; Durand, Géraldine. / Microbial typing by matrix-assisted laser desorption ionization-time of flight mass spectrometry : Do we need guidance for data interpretation?. In: Journal of Clinical Microbiology. 2015 ; Vol. 53, No. 3. pp. 760-765.
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