Microbial typing by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Do we need guidance for data interpretation?

Sébastien Spinali, Alex Van Belkum, Richard V. Goering, Victoria Girard, Martin Welker, Marc Van Nuenen, David H. Pincus, Maud Arsac, Géraldine Durand

Research output: Contribution to journalReview article

44 Citations (Scopus)

Abstract

The integration of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) in clinical microbiology has revolutionized species identification of bacteria, yeasts, and molds. However, beyond straightforward identification, the method has also been suggested to have the potential for subspecies-level or even type-level epidemiological analyses. This minireview explores MALDI-TOF MS-based typing, which has already been performed on many clinically relevant species. We discuss the limits of the method's resolution and we suggest interpretative criteria allowing valid comparison of strain-specific data. We conclude that guidelines for MALDI-TOF MS-based typing can be developed along the same lines as those used for the interpretation of data from pulsed-field gel electrophoresis (PFGE).

Original languageEnglish
Pages (from-to)760-765
Number of pages6
JournalJournal of Clinical Microbiology
Volume53
Issue number3
DOIs
StatePublished - Mar 1 2015

Fingerprint

Mass Spectrometry
Lasers
Pulsed Field Gel Electrophoresis
Microbiology
Fungi
Yeasts
Guidelines
Bacteria

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Microbial typing by matrix-assisted laser desorption ionization-time of flight mass spectrometry : Do we need guidance for data interpretation? / Spinali, Sébastien; Van Belkum, Alex; Goering, Richard V.; Girard, Victoria; Welker, Martin; Van Nuenen, Marc; Pincus, David H.; Arsac, Maud; Durand, Géraldine.

In: Journal of Clinical Microbiology, Vol. 53, No. 3, 01.03.2015, p. 760-765.

Research output: Contribution to journalReview article

Spinali, Sébastien ; Van Belkum, Alex ; Goering, Richard V. ; Girard, Victoria ; Welker, Martin ; Van Nuenen, Marc ; Pincus, David H. ; Arsac, Maud ; Durand, Géraldine. / Microbial typing by matrix-assisted laser desorption ionization-time of flight mass spectrometry : Do we need guidance for data interpretation?. In: Journal of Clinical Microbiology. 2015 ; Vol. 53, No. 3. pp. 760-765.
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