@article{740d8fa120204f5c99b871d42bdca8dd,
title = "Micromolded Carbon Paste Microelectrodes for Electrogenerated Chemiluminescent Detection on Microfluidic Devices",
abstract = "Micromolded carbon paste electrodes are easily fabricated, disposable, and can be integrated into microfluidic devices to fabricate inexpensive sensors and biosensors. In this work, carbon paste microelectrodes were fabricated in poly(dimethylsiloxane) using micromolding techniques and were coupled to a microfluidic channel to fabricate electrogenerated chemiluminescence (ECL) sensors. ECL was generated using both the tris(2,2{\textquoteright}-bipyridyl)ruthenium(II)/tripropylamine system and the hydrogen peroxide/luminol system. For each of these ECL systems, the sensor fabrication method was optimized, along with key experimental parameters (applied voltage, solution flow rate, buffer species and luminol concentration). The limit of detection (S/N=3) for TPrA was approximately 2.4 μM with a linear range of 10–100 μM. For hydrogen peroxide, the LOD was approximately 11 μM and the electrodes gave a linear response between 30 μM and 200 μM hydrogen peroxide. Electrodes containing glucose oxidase were fabricated using this new method, demonstrating that glucose could be indirectly detected via generation of hydrogen peroxide by the enzymatic reaction at the micromolded biosensor.",
author = "Gross, {Erin M.} and Porter, {Laura R.} and Stark, {Nicholas R.} and Lowry, {Emily R.} and Schaffer, {Leah V.} and Maddipati, {Sai Sujana} and Hoyt, {Dylan J.} and Stombaugh, {Sarah E.} and Peila, {Sarah R.} and Henry, {Charles S.}",
note = "Funding Information: This publication was made possible by grants from the National Institute for General Medical Science (NIGMS) (5P20GM103427), a component of the National Institutes of Health (NIH), and its contents are the sole responsibility of the authors and do not necessarily represent the official views of NIGMS or NIH. The authors would also like to acknowledge funding from NASA (Nebraska Space Grant – Mini Grant), the Creighton University Ferlic Summer Research Scholarship, and the Clare Boothe Luce Foundation. The authors would also like to thank Rachel M. Feeny, John B. Wydallis, and Meghan M. Mensack for providing the molds for fabricating microfluidic devices and electrodes. We would also like to thank the Creighton Chemistry Department and the College of Arts and Sciences. Funding Information: This publication was made possible by grants from the National Institute for General Medical Science (NIGMS) (5P20GM103427), a component of the National Institutes of Health (NIH), and its contents are the sole responsibility of the authors and do not necessarily represent the official views of NIGMS or NIH. The authors would also like to acknowledge funding from NASA (Nebraska Space Grant ? Mini Grant), the Creighton University Ferlic Summer Research Scholarship, and the Clare Boothe Luce Foundation. The authors would also like to thank Rachel M. Feeny, John B. Wydallis, and Meghan M. Mensack for providing the molds for fabricating microfluidic devices and electrodes. We would also like to thank the Creighton Chemistry Department and the College of Arts and Sciences.",
year = "2020",
month = aug,
day = "3",
doi = "10.1002/celc.202000366",
language = "English (US)",
volume = "7",
pages = "3244--3252",
journal = "ChemElectroChem",
issn = "2196-0216",
publisher = "John Wiley and Sons Ltd",
number = "15",
}